Serum trypsin-inhibitory activity in five species of farmed fish

Bowden, Timothy J.; Bütler, R; Bricknell, Ian; Ellis, A.E.

doi:10.1006/fsim.1997.0092

Cited by 82 publications

(51 citation statements)

References 13 publications

(20 reference statements)

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“…Anti-protease and haemolytic activity are important innate defense parameters of fish, including cod (Bowden et al, 1997;Sakai, 1992;Magnadóttir et al, 1999a, b). Di#erent serum components can contribute to the antiprotease activity of fish serum including 2 -macroglobulin, which according to Ellis (1990), is only stable for about 3 weeks at 20 C. For practical reasons the analyses of anti-protease activity was carried out about 10-12 months after sampling.…”

Section: Immune Parameters Of Immunised Codmentioning

confidence: 99%

Immune parameters of immunised cod ( L.)

Magnadóttir

Jónsdóttir

Helgason

et al. 2001

Fish & Shellfish Immunology

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Section: Immune Parameters Of Immunised Codmentioning

confidence: 99%

Immune parameters of immunised cod ( L.)

Magnadóttir

Jónsdóttir

Helgason

et al. 2001

Fish & Shellfish Immunology

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“…Serum total peroxidase activity The total peroxidase content of serum was determined colorimetrically as described in our earlier protocol (Rajendran et al, 2016). Serum antiprotease activity Serum antiprotease assay was performed colorimetrically according to the protocol described by Bowden et al (1997).…”

mentioning

confidence: 99%

Modulation of the innate immune responses in the striped snakehead murrel, <i>Channa striata</i> upon experimental infection with live and heat killed <i>Aeromonas hydrophila</i>

Priyadarshini¹,

Subramani²,

Michael³

2017

Open Vet J.

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It is well-known that the innate immune mechanisms in fish serve as the first line of defence against wide variety of pathogens. In most of the situations, innate responses get induced and enhanced after the pathogen invasion. It would be interesting to look into the inducibility of various innate immune mechanisms and the level of enhancement after infection with the pathogen. Hence, in the present investigation, modulation of the innate immune responses in the striped snakehead murrel, Channa striata on experimental challenge with either live virulent or heat killed Aeromonas hydrophila at a dose of 1x10 7 CFU (suspended in 0.2 mL PBS) were measured. Most of the non-specific (both humoral and cellular) immune responses tested were substantially induced or enhanced in both the experimental groups in comparison with the unchallenged control group. Significant increase in the lysozyme, total peroxidase, antiprotease and respiratory burst activities were observed after the pathogen challenge. Thus, most of the innate non-specific immune responses are inducible though they are constitutive of fish immune system exhibiting a basal level of activity even in the absence of pathogen challenge.

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“…The anti-protease activity of seminal plasma was evaluated by the inhibition of cod trypsin activity (Ciereszko et al, , 1996aBowden et al, 1997). Inhibitory activities were expressed as unit per litre (U/l).…”

Section: Methodsmentioning

confidence: 99%

Proteolytic and anti-proteolytic activity in the seminal plasma of Eurasian perch (Perca fluviatilis L.) during the spawning period

Król¹,

Kowalski²,

Demska‐Zakęś³

et al. 2011

CZECH J ANIM SCI

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ABSTRACT:The aim of this study was to describe possible changes in protease and anti-protease activity in the seminal plasma of European perch (Perca fluviatilis L.) during the spawning time. No significant difference in proteolytic activity was observed between the beginning and the late period of the spawning season in perch. Anti-protease activity significantly increased during the sampling time. Electrophoretic profiles of gelatinolytic activity in the seminal plasma of perch were characterized by four molecular forms, which depend on the presence of Ca 2+ during incubation. We also found two forms of caseinolytic activity with low molecular weights, which were independent of calcium ions. However, both activities were fully stopped by the chelator of calcium ions (EDTA). In this study, non-typical profiles of gelatinolytic activity were also observed. Profiles of protease activities in the perch seminal plasma are constant during the reproduction season indicating that the regulation of protease activity in seminal plasma occurs via protease inhibitors which are abundant in this fluid. Results concerning electrophoresis revealed at least seven forms of anti-proteases in the seminal plasma of perch. Concluded, anti-proteases comprise a high percentage of all proteins in the seminal plasma of perch, while the increase at the end of spawning season is probably protecting spermatozoa during the spawning time in perch testes.

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Serum trypsin-inhibitory activity in five species of farmed fish

Cited by 82 publications

References 13 publications

Immune parameters of immunised cod ( L.)

Immune parameters of immunised cod ( L.)

Modulation of the innate immune responses in the striped snakehead murrel, <i>Channa striata</i> upon experimental infection with live and heat killed <i>Aeromonas hydrophila</i>

Proteolytic and anti-proteolytic activity in the seminal plasma of Eurasian perch (Perca fluviatilis L.) during the spawning period

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