Serum miR-30c-5p is a potential biomarker for multiple system atrophy

Vallelunga, Annamaria; Iannitti, Tommaso; Dati, Giovanna; Capece, Sabrina; Maugeri, Marco; Tocci, Ersilia; Picillo, Marina; Volpe, Giovanni; Cozzolino, Autilia; Squillante, Massimo; Cicarelli, Giulio; Barone, Paolo; Pellecchia, Maria Teresa

doi:10.1007/s11033-019-04614-z

Cited by 20 publications

(23 citation statements)

References 23 publications

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“…Recently, miR-30-5p has been reported to be significantly upregulated in PD patients (Vallelunga et al, 2019 ). To further investigate the role of miR-30-5p in vivo , miR-30c-5p agomiR or antagomiR was transfected into MPTP-induced PD mice.…”

Section: Resultsmentioning

confidence: 99%

“…MiR-30c-5p could regulate inflammation (Ceolotto et al, 2017;Li et al, 2018), reactive oxygen species (Zou et al, 2017;Zhu et al, 2018;, metabolism (Gao et al, 2020), and apoptosis (Du et al, 2017). Moreover, the abnormal elevation of miR-30c-5p has been reported in PD (Vallelunga et al, 2019). MiR-30c-5p may serve as a biomarker to improve the clinical diagnosis or prognosis prediction in patients with PD, and a potential therapeutic target for the treatment of PD.…”

Section: Introductionmentioning

confidence: 99%

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MiR-30c-5p/ATG5 Axis Regulates the Progression of Parkinson’s Disease

Zhang

Chen

Chang

et al. 2021

Front. Cell. Neurosci.

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Serum miR-30c-5p correlates with Parkinson’s disease (PD), yet its role has not been illustrated. This research analyzed the function of miR-30c-5p in PD. The behavioral evaluation was performed on MPTP-treated PD mice transfected with miR-30c-5p agomiR, antagomiR, siATG5, or 3-MA (an autophagy inhibitor). Oxidative stress-related factors, miR-30c-5p, and apoptosis- and autophagy-associated proteins in brain tissues or cells were determined by molecular experiments. Tyrosine hydroxylase (TH) and dopamine metabolic markers were detected using immunofluorescence and Diode Array Detector (DAD), respectively. Effects of miR-30c-5p and its target gene Autophagy-related gene (ATG) 5 protein (ATG5) on MPP+-treated SH-SY5Y cells were determined through a series of molecular experiments. MiR-30c-5p was upregulated but ATG5 was downregulated in PD mice. MiR-30c-5p antagomiR attenuated the decrease of ATG5 in PD mice. MiR-30c-5p antagomiR partly alleviated the behavioral symptoms and inhibited the increases of malondialdehyde (MDA), catalase (CAT), and SOD in PD mice. The levels of Bcl-2, dopamine, dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA), TH, and LC3 II were downregulated in PD mice, while Bax, cleaved caspase-3, P62, and LC3 I were upregulated. However, miR-30c-5p antagomiR partly reversed the levels of these factors in PD mice. 3-MA could block the effects of miR-30c-5p antagomiR on PD mice. MiR-30c-5p antagomiR attenuated apoptosis and induced autophagy in brain tissues of MPTP-treated mice by targeting ATG5. In vitro assay results also showed that silence of ATG5 reduced the protective effect of miR-30c-5p downregulation on the cells. MiR-30c-5p regulates the progression of Parkinson’s disease through attenuating ATG5-inhibited apoptosis and -induced autophagy.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

MiR-30c-5p/ATG5 Axis Regulates the Progression of Parkinson’s Disease

Zhang

Chen

Chang

et al. 2021

Front. Cell. Neurosci.

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“…MiR-30c has been reported to be elevated in patients with narcolepsy, and changes in the expression of MiR-30c in response to sleep deprivation have been observed in young patients with narcolepsy [87,150]. Although the actual targets of miR-30c have not been identified, a computational algorithm revealed many target genes that are involved in neuronal autophagy, mitophagy and the regulation of dopaminergic cell death [151]. In addition, circadian PER proteins are also predicted to be target genes of miR-30c [148], although the precise mechanisms by which miR-30c affects PD pathology and circadian rhythm are unknown.…”

Section: Parkinson's Diseasementioning

confidence: 99%

MicroRNA: A Key Player for the Interplay of Circadian Rhythm Abnormalities, Sleep Disorders and Neurodegenerative Diseases

et al. 2020

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Circadian rhythms are endogenous 24-h oscillators that regulate the sleep/wake cycles and the timing of biological systems to optimize physiology and behavior for the environmental day/night cycles. The systems are basically generated by transcription–translation feedback loops combined with post-transcriptional and post-translational modification. Recently, evidence is emerging that additional non-coding RNA-based mechanisms are also required to maintain proper clock function. MicroRNA is an especially important factor that plays critical roles in regulating circadian rhythm as well as many other physiological functions. Circadian misalignment not only disturbs the sleep/wake cycle and rhythmic physiological activity but also contributes to the development of various diseases, such as sleep disorders and neurodegenerative diseases. The patient with neurodegenerative diseases often experiences profound disruptions in their circadian rhythms and/or sleep/wake cycles. In addition, a growing body of recent evidence implicates sleep disorders as an early symptom of neurodegenerative diseases, and also suggests that abnormalities in the circadian system lead to the onset and expression of neurodegenerative diseases. The genetic mutations which cause the pathogenesis of familial neurodegenerative diseases have been well studied; however, with the exception of Huntington’s disease, the majority of neurodegenerative diseases are sporadic. Interestingly, the dysfunction of microRNA is increasingly recognized as a cause of sporadic neurodegenerative diseases through the deregulated genes related to the pathogenesis of neurodegenerative disease, some of which are the causative genes of familial neurodegenerative diseases. Here we review the interplay of circadian rhythm disruption, sleep disorders and neurodegenerative disease, and its relation to microRNA, a key regulator of cellular processes.

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“…Yang Q. et al (2019) suggested that the serum level of miRNA-133b was significantly downregulated in AD patients, and that it may serve as a novel diagnostic biomarker for AD. miRNA-30c-5p has been reported to be significantly upregulated in multiple system atrophy (MSA) patients, and Vallelunga et al (2019) considered that serum miRNA-30-5p could be a biomarker for the diagnosis and progression of MSA. Regarding CmiRNAs as diagnostic markers for MND diagnosis, relatively few studies have been published, but some breakthroughs have been made.…”

Section: Source Existence Extraction and Detection Of Mirnas And Tmentioning

confidence: 99%

Circulating MicroRNAs as Diagnostic Biomarkers for Motor Neuron Disease

Lin

Zhang

2020

Front. Neurosci.

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Motor neuron disease (MND) is a kind of neurodegenerative disease that selectively invades spinal cord anterior horn cells, brainstem motor neurons, cortical pyramidal cells and the pyramidal tract. The main clinical features are the symptoms and signs of impaired upper and lower motor neurons, manifested as muscle weakness, atrophy and pyramidal tract signs. Histopathology has shown the disappearance of pyramidal cells in the motor cortex, loss of motor neurons in the anterior horn of the spinal cord and brainstem, and degeneration of the corticospinal tract. Due to the lack of effective treatment methods, the prognosis is generally poor, so it is of great significance to confirm the diagnosis early by various means. However, the current diagnosis of MND mainly relies on the combination of clinical manifestations and neurophysiological examinations, lacking effective means of early diagnosis. Circulating microRNA (CmiRNA) is a kind of stable miRNA molecule in serum, plasma and other body fluids, which has the characteristics of distinct differential expression, sensitive detection and convenient sample collection. As a possible new biomarker of MND, CmiRNA can not only reveal the pathophysiological process of MND, but also monitor disease progression and response to drug therapy. With the development of miRNA detection technology, more and more CmiRNAs as biomarkers with potential diagnostic value have been investigated. In this review, we explored the possibility of circulating samples as different sources of biomarkers for the diagnosis of MND, analyzing the progress of CmiRNA detection techniques, and presenting potential diagnostic MND biomarkers that have been reported.

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Serum miR-30c-5p is a potential biomarker for multiple system atrophy

Cited by 20 publications

References 23 publications

MiR-30c-5p/ATG5 Axis Regulates the Progression of Parkinson’s Disease

MiR-30c-5p/ATG5 Axis Regulates the Progression of Parkinson’s Disease

MicroRNA: A Key Player for the Interplay of Circadian Rhythm Abnormalities, Sleep Disorders and Neurodegenerative Diseases

Circulating MicroRNAs as Diagnostic Biomarkers for Motor Neuron Disease

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