Serum miR-146a and miR-150 as Potential New Biomarkers for Hip Fracture-Induced Acute Lung Injury

Abstract

Background Acute lung injury (ALI) and subsequent pulmonary infection are the most severe and usually fatal complications for elderly hip fracture patients. It is necessary to find some biomarkers for early diagnosis and prognosis of it. Objective This study is aimed at examining the differential expression of miR-146a, miR-150, and cytokines (IL-6 and IL-10) between younger and elderly rats suffering from hip fracture and investigating the possible meaning of them in early diagnosis and prognosis of ALI after hip fracture. Methods and Subjects Elderly rats and younger rats were randomly divided into sham group and fracture group, respectively. Two fracture groups received hip fracture operations. The damage degree of ALI was evaluated by histological observation and pathological score. Cytokines were measured by ELISA; miR-146a and miR-150 were analysed by qRT-PCR. Results After treatment, compared with the corresponding sham groups, the pulmonary histological score, the serum miR-146a concentrations, and the cytokine (IL-6 and IL-10) levels in serum and BALF were significantly higher (the miR-150 were lower) in the fracture groups (with the exception of IL-6 of the younger fracture group at 72 h, all P < 0.05). Meanwhile, compared with the younger fracture group, the aforementioned variables were significantly higher (the miR-150 levels were lower) in the elderly fracture group (with the exception of serum IL-10 and pulmonary histological score at 8 h, all P < 0.05). The results of linear regression analysis showed that serum miR-146a and miR-150 were significantly associated with pulmonary histological score. Conclusion Hip fracture can result in significant systemic inflammation and ALI in the rats. Compared to the younger rats, the elderly rats suffered a more remarkable ALI after hip fracture. It may be related to the abnormal expression of miR-146a and miR-150. Serum miR-146a and miR-150 are potential biomarkers for diagnosis and prognosis of ALI after hip fracture.

miR‑584 and miR‑146 are candidate biomarkers for acute respiratory distress syndrome

“…Numerous studies have proven that miR-146a is closely related to inflammation (46)(47)(48). There is also considerable evidence that miR-146a is closely linked to lung diseases, including ARDS (49)(50)(51). The results of the target gene enrichment in the present study are consistent with previous results.…”

Systemic immune-inflammation index independently predicts poor survival of older adults with hip fracture: a prospective cohort study

“…Several biochemical markers of inflammation, such as levels of CRP, the soluble urokinase plasminogen activating receptor (suPAR) and ferritin, were found to be associated with 30-day mortality after hip fracture [ 15 ]. Hip fracture also led to significant systemic inflammation and acute lung injury in rat models, and the older rats suffered a more remarkable acute lung injury after hip fracture than the younger rats [ 37 ]. Although other study has found no such relationship between high inflammatory markers and 2-year mortality in hip fracture patients [ 38 ], most studies suggest that systemic inflammation may be related to the poor survival of hip fracture.…”

In vivo miRNA knockout screening identifies miR-190b as a novel tumor suppressor

“…Such screening has eventually identified three miRNAs (miR-30b, miR-146a and miR-190b) negatively regulating lung tumorigenesis. Previous studies have established the functional link between miR-146a and miR-190b and lung cancer, indicating that these two miRNAs might serve as diagnostic biomarkers [ 10 , 35 , 36 ]. The miR-190b is previously shown to be up-regulated in ERα-positive breast tumors compared to ERα-negative breast tumors [ 37 ].…”