Serum microRNA-135a-5p as an auxiliary diagnostic biomarker for colorectal cancer

Abstract

Objective The purpose of this study was to explore serum miR-135a-5p expression in colorectal cancer and examine the potential usefulness of this molecule as a biomarker for diagnosis in colorectal cancer. Methods Serum samples were collected from 60 patients with primary colorectal cancer, 40 patients with colorectal polyps and 50 healthy controls. Serum miR-135a-5p expression levels were detected by reverse transcription quantitative real-time quantitative polymerase chain reaction. Serum carcinoembryonic antigen and carbohydrate antigen 199 concentrations were detected by MODULAR ANALYTICS E170. Results The relative expression level of serum miR-135a-5p in colorectal cancer patients, colorectal polyps patients and healthy controls was 2.451 (1.107, 4.413), 0.946 (0.401, 1.942) and 0.949 (0.194, 1.415), respectively, indicating that it was significantly higher in colorectal cancer patients than that in the other two groups ( U = 351.0, 313.0, both P < 0.001). Additionally, it was significantly correlated with different degrees of tumour differentiation ( U = 215.0, P = 0.029) and different tumour stages ( U = 202.0, P = 0.013). There was no significant correlation between the relative expression of serum miR-135a-5p and carcinoembryonic antigen ( r= 0.023, P = 0.293) or carbohydrate antigen 199 ( r= 0.067, P = 0.068) in colorectal cancer patients. Compared with colorectal polyps group, AUC of serum miR-135a-5p in colorectal cancer group was 0.832 with 95% CI 0.73-0.93; compared with healthy control group, AUC was 0.875 with 95% CI 0.80-0.95. Conclusion Serum miR-135a-5p expression in colorectal cancer patients was higher than that in patients with colorectal polyps and healthy controls, suggesting that serum miR-135a-5p may prove to be an important biomarker for auxiliary diagnosis of colorectal cancer.

Candidate diagnostic miRNAs that can detect cancer in prostate biopsy

“…Many miRNAs identified as dysregulated in the present study have also been reported as altered in other cancer types, including breast cancer (miR‐374b‐5p, miR‐429, miR‐200b‐5p, miR‐200b‐3p, miR‐187‐3p, miR‐196a‐5p, miR‐183‐5p, miR‐21‐5p, and miR‐182‐5p); gastric cancer (miR‐374b‐5p, miR‐200c‐3p, miR‐18b‐5p, miR‐196a‐5p, miR‐21‐5p, and miR‐20a‐3p); ovarian cancer (miR‐141‐5p, miR‐182‐5p, miR‐483‐3p, and miR‐200c‐3p); hepatocellular carcinoma (miR‐135a‐5p, miR‐187‐3p, miR‐148a‐5p, miR‐200a‐3p, and miR‐9‐3p); colorectal cancer (miR‐9‐3p, miR‐135a‐5p, miR‐200c‐3p, miR‐200b‐3p, and miR‐182‐5p); thyroid cancer (miR‐7‐5p and miR‐9‐3p); lung cancer (miR‐200b‐5p, miR‐200b‐3p, miR‐9‐5p, miR‐200a‐3p, and miR‐21‐5p); pancreatic cancer (miR‐200b‐3p, miR‐483‐3p, and miR‐183‐5p); chronic lymphocytic leukemia (miR‐4521, miR‐7‐5p, and miR‐182‐5p); Hodgkin lymphoma (miR‐876‐5p); cervical cancer (miR‐429); head and neck squamous cell carcinoma (miR‐200b‐5p); and bladder cancer (miR‐148a‐3p) …”

Identification of A Panel of Serum microRNAs as Biomarkers for Early Detection of Lung Adenocarcinoma

“…Recent studies revealed that miRNAs are stably present in serum. The expression patterns of serum miRNAs are closely correlated with progression and prognosis in a variety of human tumors, including lung cancer, colorectal cancer, prostate cancer, gastric cancer, osteosarcoma, and so on 18-24. Circulating miRNAs could be considered as a novel promising diagnostic and prognostic biomarker for cancer screening because: (ⅰ) serum miRNAs are readily detectable by RT-qPCR, a technique that widely used in clinical laboratories; (ⅱ) blood-based biomarkers are minimally invasive for the screening of high-risk subjects and early diagnosis of cancer.…”

The expression and Clinical Significance of miRNA-135a and Bach1 in colorectal cancer

“…On the other hand, miRNA-135a showed a inhibitive role during the migration and invasion of lung cancer cells, due to targeting a transcription factor [19] . However, the functions and mechanisms of miRNA-135a during tumors are largely unknown [20][21][22][23] . Recent studies have demonstrated that miRNA-135a is up-regulated in CC cell lines SW480 and SW620, while in our study, the expression levels of miRNA-135a were significantly increased in CC tissues, which was in accordance with previous studies.…”