Serum Lipid Modification Related to Exercise and Polyunsaturated Fatty Acid Supplementation in Jumpers and Thoroughbred Horses

Piccione, Giuseppe; Arfuso, Francesca; Fazio, Francesco; Bazzano, Marilena; Giannetto, Claudia

doi:10.1016/j.jevs.2014.07.005

Cited by 28 publications

(24 citation statements)

References 19 publications

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“…This result might reflect a modification in energy metabolism consisting of an increased utilization of fatty acids with a sparing of glucose and thus a reduction in BL following the physical effort. This finding is consistent with previous studies hypothesizing an enhanced uptake of fatty acids by muscle cells in exercising horses following the administration of dietary PUFA (O'Connor et al, 2004;Piccione et al, 2014). The significant increase in Hct, RBC and Hb values recorded in both groups after each jumping course was likely due to the splenic contraction, as it is well known that the equine spleen function as reservoir of 4-12 l of red blood cells that can be released into circulation at the beginning of exercise (Hinchcliff et al, 2004).…”

Section: Discussionsupporting

confidence: 93%

Increase in erythrocyte osmotic resistance following polyunsaturated fatty acids (PUFA) supplementation in show jumper horses

et al. 2015

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Osmotic fragility test has been used to study the influence of different factors on the osmotic properties of cell membranes. Considering the importance of polyunsaturated fatty acids (PUFA) in biological functions we aimed to investigate the effects of dietary PUFA supplementation on erythrocyte osmotic fragility (EOF), blood lactate (BL), hematocrit (Hct), red blood cell (RBC), hemoglobin (Hb), and mean cell volume (MCV) in 10 jumper horses. Two events occurred prior to start supplementing horse's diet and two events occurred after 4 weeks PUFA supplementation. Five horses received the PUFA supplementation (Group A), and five served as controls (Group B). Blood samples were taken before and after each course. The statistical analysis revealed significant increase in BL, Hct, RBC and Hb following exercise (P o0.0001). However, the interaction between exercise and PUFA supplementation (P¼ 0.0083) showed PUFA-supplemented horses having a smaller rise in BL levels (P¼ 0.0107) following exercise. Significant interactions between exercise and PUFA treatment were also found on EOF levels (Po 0.05). The hemolysis curves showed PUFA-supplemented horses exhibiting a reduction in EOF compared to controls (Po 0.05). Although hemolysis never occurred at 0.9% NaCl concentration, jumping exercise determined an increase in EOF (P¼ 0.0014) at 0.8% NaCl solution. A significant interaction between exercise and PUFA treatment (P¼ 0.0022) was found showing PUFA-supplemented horses having lower EOF (P ¼0.0015) following exercise. The assessment of EOF is a suitable indicator of athletic performance. The results showed that PUFA supplementation might exert beneficial effects on the horse body system by enhancing the performance in high-level show jumpers.

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Section: Discussionsupporting

confidence: 93%

Increase in erythrocyte osmotic resistance following polyunsaturated fatty acids (PUFA) supplementation in show jumper horses

et al. 2015

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“…However, the concentration of the studied parameters fell within the physiological range of the horse (Kaneko et al 2008) and was in agreement with findings reported by Piccione et al (2008Piccione et al ( , 2014b.…”

Section: Discussionsupporting

confidence: 90%

“…It is well demonstrated that albumin and NEFAs levels are influenced by exercise (Hinchcliff et al 2004;Piccione et al 2007Piccione et al , 2014b. Albumin is the main protein of mammal serum and it is essential for the regulation and keeping of oncotic pressure or osmotic pressure necessary for the proper distribution of body fluids in the vascular compartment and in tissues (Piccione et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Dynamic modulation of platelet aggregation, albumin and nonesterified fatty acids during physical exercise in Thoroughbred horses

Arfuso

Giannetto

Giudice

et al. 2016

Research in Veterinary Science

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“…In conjunction with carbohydrates, lipids are the main substrate for energy production by equine striated muscle during exercise [1]. Changes in the plasma concentrations of nonoesterified fatty acids (NEFA) and triacylglycerols in athletic horses after supramaximal exercise suggested a relevant contribution of lipid metabolism to skeletal muscle energy production in these horses [2]. Moreover, a previous study proposed that conditioning defers the onset of fatigue in athlete horses by increasing the usage of fat as an energy source [3].…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, a previous study proposed that conditioning defers the onset of fatigue in athlete horses by increasing the usage of fat as an energy source [3]. Also, added dietary fat has been associated with faster race times and prolonged run times to fatigue in Thoroughbreds, although controversy remains regarding the intrinsic mechanism responsible for this effect [2,4,5]. To date, the effect of supramaximal exercise, conditioning training and dietary fat supplementation in lipid homeostasis of Thoroughbreds has been performed using routine laboratory tests [2][3][4][5].…”

Section: Introductionmentioning

confidence: 99%

The lipidome of Thoroughbred racehorses before and after supramaximal exercise

Sassot

Villarino

Dasgupta

et al. 2019

Equine Veterinary Journal

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Summary Background A comprehensive study of the effect of supramaximal exercise in lipid homeostasis of Thoroughbreds provides the basis for future research on the role of lipids on energy metabolism in racehorses. Objective To compare the plasma lipidome of Thoroughbreds before and after supramaximal exercise using an untargeted lipidomics approach. Study design Pilot experimental study. Methods Four Thoroughbred horses were used. The maximal oxygen consumption (VO2 max) was calculated for each horse. Horses then underwent treadmill exercise at the speed for which the oxygen requirements had been calculated to be 115% VO2 max. Plasma samples were obtained before (T0) and immediately (T1), 15 (T2) and 30 (T3) minutes post‐exercise, and evaluated using liquid chromatography/mass spectrometry. Data analysis consisted of principal component analysis and one‐way repeated measures analysis of variance. Results A total of 933 plasma lipids were detected. Supramaximal exercise‐induced significant changes in the signal intensity of 13 lipids; all ubiquitous in the organism as major components of biological membranes or energy substrates. Main limitations A treadmill was used to replicate track conditions. Also, sample size involved only four horses and the statistical analyses failed to achieve the desired power of 80%. Conclusions The findings in this pilot study suggest that supramaximal exercise induces changes in specific plasma lipids in Thoroughbred racehorses. While the biological significance of these findings remains to be determined, these results provide baseline information for future studies in lipidomics applied to equine exercise physiology. Further research is warranted to better understand the role of lipids on energy metabolism in Thoroughbred racehorses.

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Serum Lipid Modification Related to Exercise and Polyunsaturated Fatty Acid Supplementation in Jumpers and Thoroughbred Horses

Cited by 28 publications

References 19 publications

Increase in erythrocyte osmotic resistance following polyunsaturated fatty acids (PUFA) supplementation in show jumper horses

Increase in erythrocyte osmotic resistance following polyunsaturated fatty acids (PUFA) supplementation in show jumper horses

Dynamic modulation of platelet aggregation, albumin and nonesterified fatty acids during physical exercise in Thoroughbred horses

The lipidome of Thoroughbred racehorses before and after supramaximal exercise

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