Serum levels of miR-320 family members are associated with clinical parameters and diagnosis in prostate cancer patients

Abstract

We studied the association of the serum levels of the microRNA family members miR-320a/-b/-c with clinico-pathological data to assess their applicability as diagnostic biomarker in prostate cancer (PCa) patients. The levels of miR-320a/-b/-c in 3 groups were evaluated by qRT-PCR (145 patients with PCa, 31 patients with benign prostatic hyperplasia (BPH) and 19 healthy controls). The levels of the three family members of miR-320 were directly correlated within each group (P < 0.001), but they differed significantly among the three groups (P < 0.001). The serum levels of the miR-320 family members were significantly increased in older patients compared to younger patients (≤ 66 years vs. > 66 years, P ≤ 0.001). In addition, the levels of all three miR-320 family members were significantly different in patients with low tumor stage compared with those with high tumor stage (miR-320a: P = 0.034; miR-320b: P = 0.006; miR-320c: P = 0.007) and in patients with low serum PSA compared with those with high serum PSA (≤ 4 ng vs. > 4 ng; miR-320a: P = 0.003; miR-320b: P = 0.003; miR-320c: P = 0.006). The levels of these miRNAs were inversely correlated with serum PSA levels. Detection in the serum samples of PCa patients with or without PSA relapse revealed higher levels of miR-320a/-b/-c in the group without PSA relapse before/after radical prostatectomy than in that with PCa relapse.In summary, the differences among the PCa/BPH/healthy control groups with respect to miR-320a/-b/-c levels in conjunction with higher levels in patients without a PSA relapse than in those with a relapse suggest the diagnostic potential of these miRNA-320 family members in PCa patients.

Correlation of plasma exosomal microRNAs with the efficacy of immunotherapy inEGFR/ALKwild-type advanced non-small cell lung cancer

“…hsa-miR-320d and hsa-miR-320e only present in primates and humans. 42 High expression of hsa-miR-320 has been reported to be associated with negative prognosis and high risk of metastasis in ovarian cancer. 43 Overexpression of hsa-miR-320 promotes B-cell proliferation through suppressing Phosphatase and tensin homolog(PTEN) expression and promotes cyclin D1 expression.…”

Highly Sensitive Circulating MicroRNA Panel for Accurate Detection of Hepatocellular Carcinoma in Patients With Liver Disease

“…Although unclear, there might be molecular mechanisms through which cancer cells selectively secret these miRNAs into the blood. On the other hand, miR‐320b was reported as a cancer biomarker in the blood samples of patients with prostate cancer and lung squamous carcinoma; expression levels were associated with clinical parameters and diagnosis . Also, serum miR‐4651 levels were highly detected in patients with aflatoxin B1‐positive HCC, and higher levels of serum miR‐4651 were significantly correlated with poor prognosis .…”

Identification of Novel Circulating miRNA Biomarkers for the Diagnosis of Esophageal Squamous Cell Carcinoma and Squamous Dysplasia

“…Although miR-320c was rarely studied in ESCC, the diagnostic utility of circulating miR-320 has been investigated in glioblastoma (33). Another study also revealed the association of the serum levels of the miR-320 family members with clinical parameters and their diagnostic potential in prostate cancer (34). As for miR-638, no study evaluated the expression level of circulating miR-638 in ESCC.…”