Serum Levels of MicroRNAs miR-371-3: A Novel Class of Serum Biomarkers for Testicular Germ Cell Tumors?

Belge, Gazanfer; Dieckmann, Klaus‐Peter; Spiekermann, Meike; Balks, Thomas; Bullerdiek, Jörn

doi:10.1016/j.eururo.2012.02.037

Cited by 101 publications

(113 citation statements)

References 5 publications

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“…Serum levels of each of the three miRNAs (miR-371, miR-372, and miR-373) decreased significantly after treatment. 6 Dieckmann et al confirmed these results in a subsequent study and, most importantly, showed that the diagnostic information of the novel markers was superior to the classical tumor markers AFP and HCG (increased in four and two out of 24 TGCT patients, respectively). 5 Patients in clinical stage I also showed a decline of circulating miRNAs following orchidectomy.…”

Section: Nucleic Acids Micrornasupporting

confidence: 68%

“…5 miRNAs have the potential to qualify as biomarkers in various malignancies because they mostly reveal a high stability in body fluids. 5,6 was demonstrated in the serum of a 4-year-old boy with a yolk sac tumor and miRNA levels declined after chemotherapy. 4 This finding stimulated research enormously, and Belge et al confirmed high levels of serum miR-371-3 with a standardized quantitative real-time polymerase chain reaction technique in a small cohort (n=11) of TGCT patients; 6 unfortunately, HCG/AFP levels were not indicated in the report.…”

Section: Nucleic Acids Micrornamentioning

confidence: 99%

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Novel tumor markers in the serum of testicular germ cell cancer patients: a review

Syring¹,

Müller²,

Ellinger³

2014

CBF

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Serum tumor markers have an important role in the management of patients with testicular cancer. They are useful for diagnosis, staging and risk assessment, follow-up, evaluation of response, and early detection of relapse. Alpha-fetoprotein, human chorionic gonadotropin, and lactate dehydrogenase are established serum markers in testicular cancer, but they have a limited sensitivity. Ongoing research may lead to the identification of novel biomarkers. Therefore, we review the experimental analyses for nucleic acids, circulating tumor cells, and proteins as potential biomarkers in the serum of testicular germ cell cancer patients.

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Section: Nucleic Acids Micrornasupporting

confidence: 68%

Section: Nucleic Acids Micrornamentioning

confidence: 99%

Novel tumor markers in the serum of testicular germ cell cancer patients: a review

Syring¹,

Müller²,

Ellinger³

2014

CBF

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“…Recently, microRNAs (miRs) have been suggested to be a novel class of serum biomarkers [10,11,12]. Regarding testicular GCT, miRs-371-3 as well as miR-302 and miR-367 represent promising candidates [13,14,15,16,17,18,19,20]. …”

Section: Introductionmentioning

confidence: 99%

MicroRNA miR-371a-3p - A Novel Serum Biomarker of Testicular Germ Cell Tumors: Evidence for Specificity from Measurements in Testicular Vein Blood and in Neoplastic Hydrocele Fluid

Dieckmann

Spiekermann²,

Balks³

et al. 2016

Urol Int

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Background: microRNAs (miRs)-371-3 are suggested to be novel biomarkers of germ cell tumors (GCTs), but their specificity is unresolved. We aimed at clarifying the origin of miR 371a-3p by measuring this miR in peripheral vein blood, and in fluids present in the vicinity of GCTs. Methods: miR-371a-3p levels were measured by quantitative PCR in 9 tumor surrounding hydroceles and in cubital vein blood (CVB) and testicular vein blood (TVB) of 64 GCT patients, 51 with clinical stage (CS) 1, 13 with CS2-3. Thirty three CS1 cases had also postoperative CVB measurement. TVB miR levels were compared with those of CVB. Associations with clinical factors were analyzed statistically. Results: TVB miR levels were 294-fold, 80-fold and 4.6-fold higher than those in CVB of CS1 patients, CS2-3 patients and controls, respectively. Neoplastic hydrocele fluid comprised of very high miR levels. In CS1, miR levels dropped to normal postoperatively. Statistically, CVB miR levels are significantly associated with tumor size (p = 0.0211) and testis length (p = 0.0493). TVB miR levels are associated with testis length (p = 0.0129). Conclusions: This study provides evidence for the origin of circulating miR 371a-3p molecules from GCT cells. miR-371a-3p represents a specific serum biomarker for germ cell cancer.

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“…Interestingly, a case report (Murray et al, 2011) showed the feasibility of measuring serum levels of the miR-371-3 and miR-302 clusters in a single patient. In addition, a decline of these miR level in serum of GCC patients after surgical removal of the (stage I) GCC was shown (Belge et al, 2012). A subsequent study (Murray and Coleman, 2012) demonstrated elevation of these miR clusters in serum of multiple patients at the time of initial diagnosis with different histological subtypes.…”

Section: Detection Of Mir-373 Mir-302c and 367 Clusters In Serum Of mentioning

confidence: 97%

Role of stem cell proteins and microRNAs in embryogenesis and germ cell cancer

Eini¹,

Dorssers²,

Looijenga³

2013

Int. J. Dev. Biol.

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Serum Levels of MicroRNAs miR-371-3: A Novel Class of Serum Biomarkers for Testicular Germ Cell Tumors?

Cited by 101 publications

References 5 publications

Novel tumor markers in the serum of testicular germ cell cancer patients: a review

Novel tumor markers in the serum of testicular germ cell cancer patients: a review

MicroRNA miR-371a-3p - A Novel Serum Biomarker of Testicular Germ Cell Tumors: Evidence for Specificity from Measurements in Testicular Vein Blood and in Neoplastic Hydrocele Fluid

Role of stem cell proteins and microRNAs in embryogenesis and germ cell cancer

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