Serum heat shock protein 27 antigen and antibody levels appear to be related to the macrovascular complications associated with insulin resistance: a pilot study

Abstract

Heat shock protein 27 (Hsp27) is over-expressed when cells are exposed to stressful conditions that include oxidative stress. Oxidative stress has been implicated in the pathogenesis of cardiovascular disease (CVD), diabetes and insulin resistance. We have investigated the concentrations of serum Hsp27 antigen and antibodies in subjects from different glycaemic categories, who either did or did not have established CVD. Serum Hsp27 antigen and antibody levels (immunoglobulins M and G (IgM and IgG)) were determined by enzyme-linked immunosorbent assays (ELISAs) in 68 individuals: 26 with normal glucose tolerance (NGT), 10 with (+) and 16 without (-) a history of CVD and 42 individuals with varying degrees of glucose intolerance (GI; 21 with and 21 without a history of CVD). Insulin sensitivity was determined in each subject using indices derived from the homeostasis model assessment of sensitivity and the insulin sensitivity index for glycaemia. Serum Hsp27 concentrations were significantly higher in GI (+CVD) subjects compared to GI (-CVD) subjects (p = 0.03), NGT (-CVD) subjects (p = 0.02) and NGT (+CVD) subjects (p = 0.04) and were positively correlated to fasting plasma glucose for all subjects (r = 0.28, p = 0.03). IgM antibody levels were significantly higher in GI (+CVD) subjects compared to NGT (-CVD) group (p = 0.02) and were inversely related to fasting insulin concentrations (r = -0.27, p = 0.04) and the 2-h insulin concentrations (r = -0.29, p = 0.03) for all subjects. Serum IgG antibody levels were higher in GI (+CVD) group compared to GI (-CVD) group (p = 0.06). In conclusion, Hsp27 and its antibody concentrations appear to relate to the presence of cardiovascular complications in patients with GI.

Serum antibody titers against heat shock protein 27 are associated with the severity of coronary artery disease

“…In addition, serum anti-Hsp27 antigen and antibody titers (IgM and IgG) have been reported to be related to the presence of cardiovascular complications in patients with glucose intolerance. This latter finding is consistent with the present findings as we have found a significant difference in the prevalence of diabetes mellitus among the groups, with the highest rate in the CAD + group (Pengiran Burut et al 2010). In the present study, serum anti-Hsp 27 titers in the CAD − group were higher than those in the control subjects but lower than those of CAD + group.…”

Impact of Exercise and Metabolic Disorders on Heat Shock Proteins and Vascular Inflammation

“…Restriction stress increases the production of Hsp70, MCP-1, PAI-1, and monocyte adhesion but decreases adiponectin in mice [163]. The levels of Hsp27 antigen and antibody in serum of diabetic patients are associated with cardiovascular complications and insulin resistance [164]. Oxidized LDL (oxLDL) has been considered as a circulating marker for coronary artery disease [165].…”

Is there any association between Serum anti-HSP27 antibody level and the presence of metabolic syndrome; population based case-control study

“…They suggested that the release of HSP27 IgM antibody into circulation may lead to formation of immune complexes. This may be one reason for the clearance of released HSP27 in patients with acute coronary syndromes (32). We did not evaluate Hsp27 IgM antibody titers in this study but found similar serum HSP27 IgG antibody levels in MetS+ and MetS-group.…”