Serum from calorie-restricted animals delays senescence and extends the lifespan of normal human fibroblasts in vitro

Cabo, Rafael de; Liu, Lijuan; Ali, Ahmed; Price, Nathan D.; Zhang, Jing; Wang, Mingyi; Lakatta, Edward G.; Irusta, Pablo M.

doi:10.18632/aging.100719

Cited by 18 publications

(12 citation statements)

References 118 publications

(100 reference statements)

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“…In the present study, SIRT1 levels in vitro as HA-VSMCs were decreased and senescence was induced. This is consistent with previous studies concerning the reduction of SIRT1 levels in senescent human VSMCs, endothelial cells and fibroblasts (25,44,45). Interestingly, when these senescent HA-VSMCs were cultured in the presence of BYHWS, much higher SIRT1 was induced than the same cells cultured in CS.…”

Section: Discussionsupporting

confidence: 92%

“…A low SIRT1 level contributed to not only an acceleration of cellular senescence but also increased capacity for cellular migration and invasion (25)(26)(27)(28)33). Thus we measured the levels of SIRT1 protein in the Ang II-induced HA-VSMCs.…”

Section: Byhws Delayed the Down-regulation Of Sirt1 Protein In Senescmentioning

confidence: 99%

“…Exposure of new VSMCs to Ang II via activation of MMP-2 increases the invasive capacity of old cells whereas MMP inhibitor reverses this effect (23,24). Emerging evidence points to sirtuin1 (SIRT1) as a contributer to the regulation of health and lifespan (25,26). It has been demonstrated that over-expression of SIRT1 and an activator of SIRT1 (e.g.…”

Section: Introductionmentioning

confidence: 99%

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Serum containing Buyang Huanwu decoction prevents age-associated migration and invasion of human vascular smooth muscle cells by up regulating SIRT1 expression

Zhang

Wei

Ruan

et al. 2018

BST

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Section: Discussionsupporting

confidence: 92%

Section: Byhws Delayed the Down-regulation Of Sirt1 Protein In Senescmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Serum containing Buyang Huanwu decoction prevents age-associated migration and invasion of human vascular smooth muscle cells by up regulating SIRT1 expression

Zhang

Wei

Ruan

et al. 2018

BST

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“…AEC-CM delays the onset of senescence without extending the lifespan of cells, possibly because the AEC secretome impedes the formation of inflammation-associated processes that induce senescence. Earlier reports have indicated delayed senescence in normal fibroblasts using serum from calorie restricted animals [63], rapamycin [64], and resveratrol [65] treatment. These findings open new perspectives for the use of AECs in regenerative medicine.…”

Section: Discussionmentioning

confidence: 99%

Conditioned medium derived from rat amniotic epithelial cells confers protection against inflammation, cancer, and senescence

et al. 2016

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Amniotic epithelial cells (AECs) are a class of fetal stem cells that derives from the epiblast and resides in the amnion until birth. AECs are suitable candidates for regenerative medicine because of the ease of collection, their low immunogenicity and inability to form tumors after transplantation. Even though human AECs have been widely investigated, the fact remains that very little is known about AECs isolated from rat, one of the most common animal models in medical testing. In this study, we showed that rat AECs retained stemness properties and plasticity, expressed the pluripotency markers Sox2, Nanog, and Oct4 and were able to differentiate toward the osteogenic lineage. The addition of conditioned medium collected from rat AECs to lipopolysaccharide-activated macrophages elicited anti-inflammatory properties through a decrease of Tnfa expression and slowed tumor cell proliferation in vitro and in vivo. The senescence-associated secretory phenotype was also significantly lower upon incubation of senescent human IMR-90 fibroblast cells with conditioned medium from rat AECs. These results confirm the potential of AECs in the modulation of inflammatory mechanisms and open new therapeutic possibilities for regenerative medicine and anti-aging therapies as well.

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“…A clue to this apparent discrepancy comes from studies using conditioned media; or more specifically, cells exposed to media supplemented with serum collected from rodents undergoing CR are more stress resistant than are cells grown in the presence of normal media alone. This suggests the presence of specific circulating factors needed for the life extending effects of dietary restriction that are lost during the derivation and expansion of individual cell lines (de Cabo et al 2015, de Cabo et al 2003.…”

Section: Primary Cell Culture Stress Resistance and Rodent Models Ofmentioning

confidence: 99%

Comparative cellular biogerontology: Where do we stand?

Alper

Bronikowski

Harper

2015

Experimental Gerontology

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Due to the extreme variation in life spans among species, using a comparative approach to address fundamental questions about the aging process has much to offer. For example, maximum life span can vary by as much as several orders of magnitude among taxa. In recent years, using primary cell lines cultured from species with disparate life spans and aging rates has gained considerable momentum as a means to dissect the mechanisms underlying the variation in aging rates among animals. In this review, we reiterate the strengths of comparative cellular biogerontology, as well as provide a survey of the current state of the field. By and large this work sprang from early studies using cell lines derived from long-lived mutant mice. Specifically, they suggested that an enhanced resistance to cellular stress was strongly associated with increased longevity of select laboratory models. Since then, we and others have shown that the degree of stress resistance and species longevity is also correlated among cell lines derived from free-living populations of both mammals and birds, and more recent studies have begun to reveal the biochemical and physiological underpinnings to these differences. The continued study of cultured cell lines from vertebrates with disparate life spans is likely to provide considerable insight toward unifying mechanisms of longevity assurance.

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Serum from calorie-restricted animals delays senescence and extends the lifespan of normal human fibroblasts in vitro

Cited by 18 publications

References 118 publications

Serum containing Buyang Huanwu decoction prevents age-associated migration and invasion of human vascular smooth muscle cells by up regulating SIRT1 expression

Serum containing Buyang Huanwu decoction prevents age-associated migration and invasion of human vascular smooth muscle cells by up regulating SIRT1 expression

Conditioned medium derived from rat amniotic epithelial cells confers protection against inflammation, cancer, and senescence

Comparative cellular biogerontology: Where do we stand?

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