Serum-free cell culture: a unifying approach

Barnes, David W.; Sato, Gordon

doi:10.1016/0092-8674(80)90540-1

Cited by 905 publications

(318 citation statements)

References 34 publications

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“…As many cells require insulin, transferrin, and selenite,88 ITS, a supplement that contains a mixture of these three substances, has become commercially available. In addition, Hiroki Murakami et al.…”

Section: History Of Cell Culture Mediamentioning

confidence: 99%

Animal‐cell culture media: History, characteristics, and current issues

Yao

Asayama

2017

Reprod Medicine & Biology

331

249

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BackgroundCell culture technology has spread prolifically within a century, a variety of culture media has been designed. This review goes through the history, characteristics and current issues of animal‐cell culture media.MethodsA literature search was performed on PubMed and Google Scholar between 1880 and May 2016 using appropriate keywords.ResultsAt the dawn of cell culture technology, the major components of media were naturally derived products such as serum. The field then gradually shifted to the use of chemical‐based synthetic media because naturally derived ingredients have their disadvantages such as large batch‐to‐batch variation. Today, industrially important cells can be cultured in synthetic media. Nevertheless, the combinations and concentrations of the components in these media remain to be optimized. In addition, serum‐containing media are still in general use in the field of basic research. In the fields of assisted reproductive technologies and regenerative medicine, some of the medium components are naturally derived in nearly all instances.ConclusionsFurther improvements of culture media are desirable, which will certainly contribute to a reduction in the experimental variation, enhance productivity among biopharmaceuticals, improve treatment outcomes of assisted reproductive technologies, and facilitate implementation and popularization of regenerative medicine.

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Section: History Of Cell Culture Mediamentioning

confidence: 99%

Animal‐cell culture media: History, characteristics, and current issues

Yao

Asayama

2017

Reprod Medicine & Biology

331

249

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“…Despite numerous efforts over the past 30 years to develop serum-free formulations (Barnes and Sato 1980;Hewlett et al 1989) most mammalian cells in the research setting are still grown routinely in media supplemented with 5% fetal calf or fetal bovine serum (FCS/FBS). One of the main factors is the lack of a universal replacement for serum that could support the growth of the variety of cells that serum does.…”

Section: Serum Supplementationmentioning

confidence: 99%

“…The most commonly used chelator of iron in SFM is transferrin (Barnes and Sato 1980). Iron is required by eukaryotic and prokaryotic cells where it supports metabolic processes (Richardson and Baker 1994) and cell growth (Laskey et al 1988).…”

Section: Transferrinmentioning

confidence: 99%

The role of recombinant proteins in the development of serum-free media

2006

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Early developments in serum-free media led to a variety of formulations in which components normally provided in serum and required for growth (insulin, transferrin, lipid supplements, trace elements) and poorly defined components (extracts, hydrolysates) were added to defined basal media. These additives were mostly animal-derived. Given recent concerns about TSEs (transmissible spongiform encephalopathies) and other adventitious agents, the drive in media formulations must be towards elimination of animal-origin materials while maintaining cell line productivity. The progress made towards removing animal-derived components and the use of recombinant proteins in serum-free media for mammalian cells is reviewed.

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“…In parallel Sato and his colleagues came to realize that a major function of serum in cell cultures was to provide peptide hormones and growth factors (Sato 1975). This line of thought led to the formulation of cell type-specific hormonally-defined serum-free media (Barnes and Sato 1980) and to the discovery of novel polypeptide growth factors, which themselves have been incorporated into cell culture media for specific cell types.…”

Section: Introductionmentioning

confidence: 99%

Biomedical advances from tissue culture

Okamoto

Sato²,

Barnes

et al. 2013

Cytotechnology

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The demonstration that the ''dedifferentiation'' of cells commonly observed in the early days of tissue culture was due to selective overgrowth of fibroblasts led to enrichment culture techniques (alternate animal and culture passage) designed to give a selective advantage to functionally differentiated tumor cells. These experiments resulted in the derivation of a large number of functionally differentiated clonal strains of a range of cell types. These results gave rise to the hypothesis that cells in culture accurately represent cells in vivo but without the complex in vivo environment. This concept has been strengthened with the development of hormonally defined culture media in combination with functionally differentiated clonal cell lines, which have augmented the potential of tissue culture studies. The use of hormonally defined media in place of serum-supplemented media demonstrates that hormonal responses and dependencies can be discovered in culture. Discoveries of hormonal dependencies of cancer cells has led to therapies targeting intracellular signaling pathways while discoveries of hormonal responses of pluripotent cells are helping to identify the potential application of stem cells. In these and other ways tissue culture technology will continue to contribute to solving problems of human health.

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Serum-free cell culture: a unifying approach

Cited by 905 publications

References 34 publications

Animal‐cell culture media: History, characteristics, and current issues

Animal‐cell culture media: History, characteristics, and current issues

The role of recombinant proteins in the development of serum-free media

Biomedical advances from tissue culture

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