Serum Carnitine Levels in Newborns with Perinatal Asphyxia and Relation to Neurologic Prognosis

Ezgu, Fatih Süheyl; Atalay, Yıldız; Hasanoğlu, Alev; Gücüyener, Kıvılcım; Biberoğlu, Gürsel; Koç, Esin; Ergenekon, Ebru; Tümer, Leyla

doi:10.1080/10284150400017280

Cited by 5 publications

(3 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Lysine is an essential amino acid mainly involved as a precursor in protein biosynthesis; in addition, lysine is a precursor in the biosynthesis of free carnitine and its esterified form acylcarnitine, two key metabolites for β-oxidation. In hypoxia-ischemia, the decrease of fatty acid oxidation causes an increase in carnitines concentrations (45). At birth we found very high levels of urinary lysine probably correlated with the increasing need of energy source by cells during hypoxia.…”

Section: Discussionmentioning

confidence: 61%

Urinary gas chromatography mass spectrometry metabolomics in asphyxiated newborns undergoing hypothermia: from the birth to the first month of life

Noto¹,

Pomero²,

Mussap³

et al. 2016

Ann. Transl. Med.

View full text Add to dashboard Cite

Background: Perinatal asphyxia is a severe clinical condition affecting around four million newborns worldwide.It consists of an impaired gas exchange leading to three biochemical components: hypoxemia, hypercapnia and metabolic acidosis. Methods:The aim of this longitudinal experimental study was to identify the urine metabolome of newborns with perinatal asphyxia and to follow changes in urine metabolic profile over time. Twelve babies with perinatal asphyxia were included in this study; three babies died on the eighth day of life. Total-body cooling for 72 hours was carried out in all the newborns. Urine samples were collected in each baby at birth, after 48 hours during hypothermia, after the end of the therapeutic treatment (72 hours), after 1 week of life, and finally after 1 month of life. Urine metabolome at birth was considered the reference against which to compare metabolic profiles in subsequent samples. Quantitative metabolic profiling in urine samples was measured by gas chromatography mass spectrometry (GC-MS). The statistical approach was conducted by using the multivariate analysis by means of principal component analysis (PCA) and orthogonal partial least square discriminant analysis (OPLS-DA). Pathway analysis was also performed. Results:The most important metabolites depicting each time collection point were identified and compared each other. At birth before starting therapeutic hypothermia (TH), urine metabolic profiles of the three babies died after 7 days of life were closely comparable each other and significantly different from those in survivors. Conclusions:In conclusion, a plethora of data have been extracted by comparing the urine metabolome at birth with those observed at each time point collection. The modifications over time in metabolites composition and concentration, mainly originated from the depletion of cellular energy and homeostasis, seems to constitute a fingerprint of perinatal asphyxia.

show abstract

Section: Discussionmentioning

confidence: 61%

Urinary gas chromatography mass spectrometry metabolomics in asphyxiated newborns undergoing hypothermia: from the birth to the first month of life

Noto¹,

Pomero²,

Mussap³

et al. 2016

Ann. Transl. Med.

View full text Add to dashboard Cite

show abstract

“…In this study, we have evaluated alterations to ACP in neonates with perinatal asphyxia, investigated the possible effect of therapeutic hypothermia on these patterns, and analyzed the potential prognostic role of individual acylcarnitines in HIE. Previous studies that have assessed carnitine or acylcarnitine profiles using blood samples collected from patients with HIE in the perinatal period (days 1–7 of life) have confirmed decreased total and free carnitine levels, increased acylcarnitine levels, [31,32] and an increased acylcarnitine:total carnitine ratio. [25] Findings from animal models suggest that these alterations could be linked to a decrease in carnitine-palmitoyl transferase I and II and a concomitant increase in the acylcarnitine:free carnitine ratio.…”

Section: Discussionmentioning

confidence: 93%

Acylcarnitine profile in neonatal hypoxic-ischemic encephalopathy

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Studies of carnitine, and acylcarnitine profiles, in perinatal asphyxia using post-natal blood samples (collected from Day 1–7) have yielded conflicting results [60], [61], [62]. This is unsurprising as post-natal samples may be altered secondary to resuscitation [25], or the infant’s method of feeding, making their analysis unreliable.…”

Section: Discussionmentioning

confidence: 99%

The Metabolomic Profile of Umbilical Cord Blood in Neonatal Hypoxic Ischaemic Encephalopathy

et al. 2012

View full text Add to dashboard Cite

BackgroundHypoxic ischaemic encephalopathy (HIE) in newborns can cause significant long-term neurological disability. The insult is a complex injury characterised by energy failure and disruption of cellular homeostasis, leading to mitochondrial damage. The importance of individual metabolic pathways, and their interaction in the disease process is not fully understood. The aim of this study was to describe and quantify the metabolomic profile of umbilical cord blood samples in a carefully defined population of full-term infants with HIE.Methods and FindingsThe injury severity was defined using both the modified Sarnat score and continuous multichannel electroencephalogram. Using these classification systems, our population was divided into those with confirmed HIE (n = 31), asphyxiated infants without encephalopathy (n = 40) and matched controls (n = 71). All had umbilical cord blood drawn and biobanked at −80°C within 3 hours of delivery. A combined direct injection and LC-MS/MS assay (AbsolutIDQ p180 kit, Biocrates Life Sciences AG, Innsbruck, Austria) was used for the metabolomic analyses of the samples. Targeted metabolomic analysis showed a significant alteration between study groups in 29 metabolites from 3 distinct classes (Amino Acids, Acylcarnitines, and Glycerophospholipids). 9 of these metabolites were only significantly altered between neonates with Hypoxic ischaemic encephalopathy and matched controls, while 14 were significantly altered in both study groups. Multivariate Discriminant Analysis models developed showed clear multifactorial metabolite associations with both asphyxia and HIE. A logistic regression model using 5 metabolites clearly delineates severity of asphyxia and classifies HIE infants with AUC = 0.92. These data describe wide-spread disruption to not only energy pathways, but also nitrogen and lipid metabolism in both asphyxia and HIE.ConclusionThis study shows that a multi-platform targeted approach to metabolomic analyses using accurately phenotyped and meticulously biobanked samples provides insight into the pathogenesis of perinatal asphyxia. It highlights the potential for metabolomic technology to develop a diagnostic test for HIE.

show abstract

Serum Carnitine Levels in Newborns with Perinatal Asphyxia and Relation to Neurologic Prognosis

Cited by 5 publications

References 17 publications

Urinary gas chromatography mass spectrometry metabolomics in asphyxiated newborns undergoing hypothermia: from the birth to the first month of life

Urinary gas chromatography mass spectrometry metabolomics in asphyxiated newborns undergoing hypothermia: from the birth to the first month of life

Acylcarnitine profile in neonatal hypoxic-ischemic encephalopathy

The Metabolomic Profile of Umbilical Cord Blood in Neonatal Hypoxic Ischaemic Encephalopathy

Contact Info

Product

Resources

About