Serum 8-hydroxy-2-deoxyguanosine as a marker of DNA oxidative damage in horses with recurrent airway obstruction

Abstract

BackgroundIt has been reported that equine recurrent airway obstruction (RAO) is a state of oxidative stress. Oxidant-antioxidant imbalance is known to increase the conversion of deoxyguanosine to 8-hydroxy-2-deoxyguanosine (8-OHdG) in DNA. 8-OHdG can easily be measured using ELISA tests in serum or urine samples. In this study, we analysed serum 8-OHdG levels in horses with recurrent airway obstruction and in healthy controls.ResultsThe study material consisted of seven healthy horses and seven horses with symptomatic RAO. All horses were exposed to moldy hay and straw for 48 h to induce clinical exacerbation of RAO. The serum 8-OHdG levels were determined using the ELISA Highly Sensitive 8-OHdG kit. The difference between the levels of 8-OHdG in healthy and RAO-affected horses was significant. The median level of 8-OHdG was 0.044 ng/ml in the healthy controls versus 0.498 ng/ml in RAO horses (P = 0.0021).ConclusionsThe results of the study strongly suggest that DNA damage coexists in the course of equine RAO. We therefore propose that future research should aim at the development of new drugs that target pro-inflammatory molecules, since DNA damage appears to be the result of chronic inflammation.

Effect of glutathione S-transferase gene polymorphisms on semen quality in patients with idiopathic male infertility

“…The levels of 8-OHdG were evaluated using a commercial enzyme-linked immunosorbent assay (ELISA) kit (Highly Sensitive 8-OHdG Check ELISA; Fukuroi, Shizuoka, Japan), as previously described. 24 – 25 …”

“…The levels of 8-OHdG were evaluated using a commercial enzyme-linked immunosorbent assay (ELISA) kit (Highly Sensitive 8-OHdG Check ELISA; Fukuroi, Shizuoka, Japan), as previously described. [24][25] Sperm chromatin structure assay (SCSA) SCSA measures the susceptibility of sperm DNA to acid-induced denaturation in situ by using the metachromatic properties of acridine orange (AO). By quantifying this metachromatic shift of AO from green to red after acid treatment using flow cytometry, the extent of DNA denaturation can be determined.…”

Relationship between Oxidative Stress and Endometritis: Exploiting Knowledge Gained in Mares and Cows

“…Previously, 8-OHdG levels were measured by HPLC with electrochemical detection (HPLC-EC) [ 70 ]; actually, ELISA kits detecting 8-OHdG are available allowing the use of this biomarker for investigating the OS involvement in many human diseases [ 72 ]. Additionally, in veterinary medicine, 8-OHdG is more and more assessed for evaluating OS-mediated DNA damage in either biotic or abiotic diseases [ 73 , 74 , 75 ].…”

Measurement of 8‐hydroxy‐2′‐deoxyguanosine in serum and cerebrospinal fluid of horses with neuroaxonal degeneration and other causes of proprioceptive ataxia

“…10 Since neurodegenerative diseases display many similarities across species, our goal was to investigate 8-OHdG as a potential diagnostic biomarker that correlates with the histopathologic diagnosis of NAD/DM in the horse. The specific objectives of the study were (a) to optimize and validate a competitive ELISA previously reported in equids 14 for measuring 8-OHdG in equine serum and CSF and (b) to determine if measurable 8-OHdG in serum and CSF could aid in the antemortem diagnosis of NAD/DM. We hypothesized that 8-OHdG will be increased in serum and CSF from horses with NAD/DM compared with horses with other neurologic diseases, including cervical vertebral stenotic myelopathy (CVSM) and equine protozoal myeloencephalitis (EPM), and a control group of neurologically normal horses (control).…”