SERS-based liquid biopsy of saliva and serum from patients with Sjögren’s syndrome

Ştefancu, Andrei; Badarinza, Maria; Moisoiu, Vlad; Iancu, Ștefania D.; Şerban, Oana; Leopold, Nicolae; Fodor, Daniela

doi:10.1007/s00216-019-01969-x

Cited by 39 publications

(27 citation statements)

References 28 publications

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“…In summary, the accuracy values obtained by saliva-based vs. in comparison with those of gold-standard diagnostics, are quite comparable for cancer, results also found with other non-cancer pathologies, such as SjS [ 28 , 29 , 65 ]. Overall, the reported diagnostic measures for saliva-based vs. are promising with existing diagnostic modalities.…”

Section: Discussionsupporting

confidence: 66%

“…Sjögren’s syndrome (SjS) is a systemic autoimmune disease that is characterized by lymphoid infiltration of the salivary and lacrimal glands [ 28 ]. In 2019, Stefancu et al used SERS of saliva and serum samples to discriminate SjS ( n = 29) from control ( n = 21) subjects [ 28 ]. For both saliva and serum, SERS spectra depicted some similar bands that were attributed mainly to purine metabolites, such as uric acid, xanthine, and hypoxanthine ( Table 2 ).…”

Section: Resultsmentioning

confidence: 99%

“…In contrast and surprisingly enough, for Sjögren’s syndrome (SjS), the same group found different results with an equivalent number of patients, the same technique (SERS), and an identical prediction model (PCA-LDA followed by LOOCV). Indeed, in 2019 Stefancu et al obtained a sensitivity of 96.5%, specificity of 90.5%, and accuracy of 94% [ 28 ], while in 2020 Moisoiu et al obtained a sensitivity of 77%, specificity of 74%, and accuracy of 75% [ 29 ]. These different performances could be related to the sample preparation method (deproteinized saliva) [ 28 ] or not [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

“…Indeed, in 2019 Stefancu et al obtained a sensitivity of 96.5%, specificity of 90.5%, and accuracy of 94% [ 28 ], while in 2020 Moisoiu et al obtained a sensitivity of 77%, specificity of 74%, and accuracy of 75% [ 29 ]. These different performances could be related to the sample preparation method (deproteinized saliva) [ 28 ] or not [ 29 ]. Furthermore, the same group of researchers, in 2014, Feng et al [ 16 ] obtained an accuracy value of 90.2% and Qiu et al in 2016 [ 17 ], and accuracy value of 83.9% for nasopharynx cancer, despite an identical procedure for statistical analysis (PCA-LDA with LOOCV) and using the same approach as SERS.…”

Section: Discussionmentioning

confidence: 99%

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Vibrational Spectroscopy Saliva Profiling as Biometric Tool for Disease Diagnostics: A Systematic Literature Review

et al. 2020

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Saliva is a biofluid that can be considered as a “mirror” reflecting our body’s health status. Vibrational spectroscopy, Raman and infrared, can provide a detailed salivary fingerprint that can be used for disease biomarker discovery. We propose a systematic literature review based on the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) guidelines to evaluate the potential of vibrational spectroscopy to diagnose oral and general diseases using saliva as a biological specimen. Literature searches were recently conducted in May 2020 through MEDLINE-PubMed and Scopus databases, without date limitation. Finally, over a period of 10 years, 18 publications were included reporting on 10 diseases (three oral and seven general diseases), with very high diagnostic performance rates in terms of sensitivity, specificity, and accuracy. Thirteen articles were related to six different cancers of the following anatomical sites: mouth, nasopharynx, lung, esophagus, stomach, and breast. The other diseases investigated and included in this review were periodontitis, Sjögren’s syndrome, diabetes, and myocardial infarction. Moreover, most articles focused on Raman spectroscopy (n = 16/18) and more specifically surface-enhanced Raman spectroscopy (n = 12/18). Interestingly, vibrational spectroscopy appears promising as a rapid, label-free, and non-invasive diagnostic salivary biometric tool. Furthermore, it could be adapted to investigate subclinical diseases—even if developmental studies are required.

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Section: Discussionsupporting

confidence: 66%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Vibrational Spectroscopy Saliva Profiling as Biometric Tool for Disease Diagnostics: A Systematic Literature Review

et al. 2020

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“…The liquid biopsy is generally referred to as the analysis of tumor-derived material from peripheral blood. However, in the general sense, this can also include sampling from other bodily fluids such as urine [ 47 ], stool [ 48 ], cerebrospinal fluid [ 49 ], saliva [ 50 ], pleural fluid [ 51 ], and ascites [ 52 ]. In this review, circulating tumor cells (CTCs) and cell-free DNA (cfDNA) derived from peripheral blood will be the focus of this discussion.…”

Section: Liquid Biopsy For Crcmentioning

confidence: 99%

Liquid Biopsy in Colorectal Carcinoma: Clinical Applications and Challenges

Kolenčík

Shishido

Pitule

et al. 2020

Cancers

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Colorectal carcinoma (CRC) is characterized by wide intratumor heterogeneity with general genomic instability and there is a need for improved diagnostic, prognostic, and therapeutic tools. The liquid biopsy provides a noninvasive route of sample collection for analysis of circulating tumor cells (CTCs) and genomic material, including cell-free DNA (cfDNA), as a complementary biopsy to the solid tumor tissue. The solid biopsy is critical for molecular characterization and diagnosis at the time of collection. The liquid biopsy has the advantage of longitudinal molecular characterization of the disease, which is crucial for precision medicine and patient-oriented treatment. In this review, we provide an overview of CRC and the different methodologies for the detection of CTCs and cfDNA, followed by a discussion on the potential clinical utility of the liquid biopsy in CRC patient care, and lastly, current challenges in the field.

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The key role of ergothioneine in label‐free surface‐enhanced Raman scattering spectra of biofluids: a retrospective re‐assessment of the literature

Fornasaro

Sergo

2022

FEBS Letters

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Label-free surface-enhanced Raman scattering (SERS) has recently gained attention in the field of liquid biopsy as a rapid and relatively inexpensive technique that could significantly ease clinical diagnosis and prognosis by investigating a biofluid sample with a laser. Indeed, SERS spectra provide information about a set of metabolites present in the analysed biofluid, thereby offering biochemical insight into specific health conditions. Ergothioneine plays a key role since it is one of the few metabolites in biofluids that are detectable by label-free SERS. In the past decade, many studies characterizing biofluids or other biological samples have unknowingly linked this amino acid with crucial metabolic processes, including inflammation, in a plethora of diseases. However, since the SERS spectrum of ergothioneine has been reported only recently, most past studies inadvertently assigned what are now recognized as the spectral features of this compound to other molecules. The purpose of the present review is to summarize and re-evaluate these studies in the light of the recent SERS characterization of ergothioneine so as to better recognize the role of ergothioneine in many clinical conditions.

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SERS-based liquid biopsy of saliva and serum from patients with Sjögren’s syndrome

Cited by 39 publications

References 28 publications

Vibrational Spectroscopy Saliva Profiling as Biometric Tool for Disease Diagnostics: A Systematic Literature Review

Vibrational Spectroscopy Saliva Profiling as Biometric Tool for Disease Diagnostics: A Systematic Literature Review

Liquid Biopsy in Colorectal Carcinoma: Clinical Applications and Challenges

The key role of ergothioneine in label‐free surface‐enhanced Raman scattering spectra of biofluids: a retrospective re‐assessment of the literature

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