Serotypes of HFRS (Haemorrhagic Fever With Renal Syndrome) Virus in East European and Far Eastern U.S.S.R.

Virus-specific proteins and RNAs from cells infected with hemorrhagic fever with renal syndrome (HFRS) viruses derived from endemic areas of the U.S.S.R.

“…2). These data support previously published serological results and indicate that there are antigenic differences between East Asian and European HFRS virus isolates [11,22,24]. On the other hand, the immunoprecipitation experiments have shown that in addition to the N protein, G 2 glycoprotein carries group-cross-reactive epitopes.…”

“…5 Mock-infected cells; 6, 7 ribosomal RNAs from E. coli and Vero cells taviruses in a high titer from the culture m e d i u m for isolation and analysis of R N A . We believe that in addition t o immunoprecipitation and serological methods [5,11,16,21,22,24,29] "electropherotyping" of nucleocapsid-associated R N A s might be used for identifying and distinguishing hantaviruses.…”

The clinical spectrum of human hantavirus infection in Somerset, UK

“…Residual antibody many years after the presumed primary infection may reflect the intensity of the original exposure [18] or chronic infection by the virus. In the past, workers identifying hantavirus infection for the first time in a country found only low levels of antibody [18,[20][21][22][23][24]. However, when sera were tested against the indigenous virus isolate, the levels were markedly higher [25].…”

Definition of Three Serotypes of Hantaviruses by a Double Sandwich ELISA with Biotin-Avidin Amplification System

“…No virus antigen was detected by IF on the surface of the cells infected with Hantaviruses. Chumakov & Gavrilovskaya (1980) and Tkachenko et al (1982) using ELISA and RIA with pools of wild rodent lung suspensions as antigen and HFRS patient sera as the source of capturing antibody, confirmed the difference in reactivity between some convalescent HFRS patient sera from the European and Far Eastern parts of the U.S.S.R. A relative difference in antibody titre was demonstrated by IF tests when various HFRS patient and animal sera were titrated against Hantaan, NE and Prospect Hill antigens . Analyses of antiviral antibody by RIA and plaque reduction neutralization tests showed that Hantaan, SR-11 and Tchoupitoulas strains are antigenically very close to each other but different from both Puumala and Prospect Hill viruses .…”