Seroprevalence of anti-HEV and HEV RNA among volunteer blood donors and patients with Hepatitis B and C in Iran

Abstract

Aim: To assess seroprevalence of antibodies to hepatitis E virus (HEV) in healthy blood donors and hepatitis B, C patients. Methods: 450 subjects consisted of 200 blood donors in Tehran blood transfusion center, 100 subjects with hepatitis C and 150 subjects with hepatitis B infection enrolled in this study. The A549 cell line was grown in mixed medium. Cells were infected with hepatitis E virus that was purified from stool sample of a patient confirmed for hepatitis E infection by reverse transcription-polymerase chain reaction (RT-PCR) method. Supernatant of infected cells was used as positive control in our RT- PCR assay. Results: In a total of 450 subjects, 33 (7.3%) had positive anti-HEV by enzyme-linked immunosorbent assay (ELISA). Anti-HEV was seen in (9/200) 4.5%, (7/100) 7%, and (17/150) 11.3% of healthy blood donors, hepatitis C, and hepatitis B subjects, respectively. Difference between two groups was statistically significance (P = 0.028). Difference between frequency of anti-HEV in hepatitis B in relation to healthy blood donors was significant (P = 0.014). Conclusions: HEV infection is more common in subjects with hepatitis B. Keywords: Hepatitis E virus, Seroprevalence, Transmission, Iran doi: 10.3329/blj.v1i1.2623 Bangladesh Liver Journal Vol.1(1) 2009 p.34-37

Seroprevalence of Hepatitis E Virus Infection in Middle Eastern Countries: A Systematic Review and Meta-Analysis

“… Forest plot meta-analysis of seroprevalence of hepatitis E virus infection in Middle Eastern countries [ 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , ...…”

Hepatitis E Virus Seroprevalence and Viremia in Hemodialysis and HIV Infected Patients in Iran

“…Although the fecal-oral route is the main route of HEV transmission, the recent studies have demonstrated transfusion of HEV infection via blood products [4][5][6]. Previous seroprevalence studies among blood donors in Iran showed variable rates of anti-HEV antibodies of 4.5-16.7% [7][8][9][10][11]. As patients undergoing chronic hemodialysis have impaired immunity and a history of blood transfusion, they are at risk of nosocomially and parenterally transmition agents [12,13].…”