Serological investigation and PCR in detection of pathogenic leptospires in snakes

Biscola, Natália P.; Fornazari, Felipe; Saad, Eduardo; Richini-Pereira, Virgínia Bodelão; Campagner, Michelle V.; Langoni, Hélio; Barraviera, Benedito; Ferreira, Rui Seabra

doi:10.1590/s0100-736x2011000900013

Cited by 29 publications

(22 citation statements)

References 15 publications

(14 reference statements)

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“…in 6.3% (1/16) of the kidney samples obtained from a wild Crotaphopeltis hotamboeia (Herald Snake) (Jobbins and Alexander, 2015); Leptospira spp. was also detected by PCR in kidney and liver samples of one (1/1) wildlife Bothrops pauloensis (Jararaca-pintada), and in kidney samples of two (2/2) captive Bothrops moojeni (Brazilian lancehead) (Biscola et al, 2011).…”

Section: Molecular Diagnosis Of Leptospiramentioning

confidence: 97%

“…The serovars detected in these specimens were Autumnalis (1:160), Copenhageni (1:80 and 1:160) and Pyrogenes (1:20 and 1:160). Biscola et al (2011) studied wildlife and captive snakes (n 3), and described the concurrent detection of the genomic material of Leptospira spp. in kidney samples and antibodies against this bacterium in serum samples (serovar Hardjo prajtino 1:200 and 1:1600).…”

Section: Microagglutination Test Versus Molecular Diagnosismentioning

confidence: 99%

“…Another sensitive and reliable diagnostic method for the detection of Leptospira spp. is polymerase chain reaction (PCR) (Acha and Szyfres, 2001), which has been successfully applied in reptiles' samples (Biscola et al, 2011;Jobbins and Alexander, 2015;Mitchell et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Detection of Leptospira spp. in wild Phrynops geoffroanus (Geoffroy’s side-necked turtle) in urban environment

et al. 2016

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Leptospira spp., a zoonotic agent relevant for public health, occurs frequently in tropical regions. The aquatic environment represents a viable survival and transmission pathway. This study aimed to investigate the presence of anti-Leptospira spp. antibodies in Phrynops geoffroanus (Geoffroy's side-necked turtle) serum samples using the microagglutination test (MAT), and Leptospira spp. in gastric and cloacal lavage samples using the polymerase chain reaction (PCR) technique. Antibodies against nine different Leptospira spp. serovars were detected in 45.45% (30/66) of the serum samples. Specific amplification of Leptospira spp. genomic material (331bp) was observed in 16.67% (11/66) of the samples. In conclusion, these freshwater testudines host Leptospira spp. and eliminate them. This situation may represent a risk to public health, especially to people who use urban streams for fishing and recreational activities. Additionally, we described some Leptospira spp. serovars, not yet reported in testudines, detected here in P. geoffroanus.

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Section: Molecular Diagnosis Of Leptospiramentioning

confidence: 97%

Section: Microagglutination Test Versus Molecular Diagnosismentioning

confidence: 99%

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Detection of Leptospira spp. in wild Phrynops geoffroanus (Geoffroy’s side-necked turtle) in urban environment

et al. 2016

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“…Em um estudo realizado com 343 morcegos capturados em diferentes áreas de São Paulo, foi confirmada a positividade para leptospiras patogênicas pela PCR, utilizando amostras de tecidos renais, o que sugere que os morcegos podem atuar como reservatórios na cidade de São Paulo 76 77 .…”

Section: Leptospirose Em Animais De Criaçãounclassified

Reservatórios Animais Da Leptospirose: Uma Revisão Bibliográfica

2013

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Resumo: Através de uma revisão bibliográfica, apresentamos algumas das principais espécies animais envolvidas no ciclo de transmissão da leptospirose. Foram lidos 80 trabalhos publicados, a busca se deu através de portais indexadores, bibliotecas, livros e informes técnicos que citam como descritores as palavras lepstospiras em animais; sorovares de leptospiras e leptospirose em animais. Assim, relatamos os achados de leptospira em espécies domésticas, de criação, sinantrópicas e silvestres. O conhecimento dos sorovares prevalentes, bem como dos hospedeiros que participam do ciclo da leptospirose é uma estratégia de grande importância que deve ser adotada para o entendimento epidemiológico da doença.Descritores: Sorovares de Leptospiras, Reservatórios Animais, Leptospirose Animal. Animal reservoirs of leptospirosis: a literature reviewAbstract: Through a literature review, we present some of the main species involved in the transmission cycle of leptospirosis. Were read 80 articles published, the research was done through portals of Indexers, libraries, books and technical reports mentioning the words as descriptors lepstospiras in animals; serovars of leptospires and leptospirosis in animals. Thus, we report the results of leptospira in domestic species of creation, synanthropic and wild. The knowledge of prevalent serovars and reservoirs that participate in the cycle of leptospirosis is an important strategy to be adopted for understanding the epidemiology of the disease.

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“…Os roedores são os principais carreadores e em teoria qualquer mamífero pode ser um reservatório em potencial (LEFEBVRE, 2004). Ainda que existam diversos relatos em aves, répteis e anfíbios a significância epidemiológica não está esclarecida (TORTEN;SHENBERG;VAN DER HOEDEN, 1965;EVERARD et al, 1988;GRAVEKAMP et al, 1991;BISCOLA et al, 2011;WHATMORE et al, 2015 Stuart et al (1977) e Motie et al (1986). No Brasil, inquéritos sorológicos foram conduzidos por Lins & Lopes (1984) em D.…”

Section: Transmissão Hospedeiros E Sinais Clínicosunclassified

<b>Pesquisa de agentes infecciosos selecionados em tatus da região do Pantanal sul-mato-grossense</b>

Dalazen¹

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Pantanal is considered one of the world's largest wetlands. Seven of the eleven species of armadillos that occur in Brazil, from a total of 21 recognized species in the world, have been recorded in this area. The main local economic activity is beef cattle production, which leads to intense wildlife-livestock contact, potentially increasing wildlife exposure to several pathogens, including those with zoonotic and economic relevance. Previous studies demonstrated that several wildlife species have been exposed to Brucella abortus and Leptospira interrogans in Pantanal; however, the exposure and/or presence of zoonotic pathogens in armadillos in this biome is still poorly understood. In order to address this question, we employed conventional polymerase chain reaction (PCR), the Rose Bengal Test (RBT) and the Microscopic Agglutination Test (MAT) to investigate the exposure and/or infection by Brucella abortus and Leptospira interrogans in blood samples of four armadillos species: nine-banded armadillo (Dasypus novemcinctus) (n=2), southern nakedtailed armadillo (Cabassous unicinctus) (n=8), yellow armadillo (Euphractus sexcinctus) (n=16) and giant armadillo (Priodontes maximus) (n=22) caught at Nhecolândia, Pantanal, Brazil. We did not detect Brucella abortus by PCR or exposure to this pathogen via the RBT in the evaluated armadillos. On the other hand, the Microscopic Agglutination Test (MAT) detected Leptospira interrogans exposure in 31.25 % (5/16) of E. sexcinctus and 18.18% (4/22) P. maximus specimens. These individuals were positive to serovars Autumnalis/Butembo, Cynopteri/Cynopteri and Pomona/Pomona, this latter with titers ranging from 1:200 to 1:1600. Our results reinforce the importance of pathogen surveillance in wildlife living in areas of livestock production and further contribute to the study of zoonotic pathogens in armadillos in the Pantanal region.

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Serological investigation and PCR in detection of pathogenic leptospires in snakes

Cited by 29 publications

References 15 publications

Detection of Leptospira spp. in wild Phrynops geoffroanus (Geoffroy’s side-necked turtle) in urban environment

Detection of Leptospira spp. in wild Phrynops geoffroanus (Geoffroy’s side-necked turtle) in urban environment

Reservatórios Animais Da Leptospirose: Uma Revisão Bibliográfica

<b>Pesquisa de agentes infecciosos selecionados em tatus da região do Pantanal sul-mato-grossense</b>

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