Serological evidence of hepatitis E virus infection in different animal species from the Southeast of Brazil

Vitral, Cláudia Lamarca; Pinto, Marcelo Alves; Lewis-Ximenez, Lia Laura; Khudyakov, Yuri; Santos, Débora R dos; Gaspar, Ana

doi:10.1590/s0074-02762005000200003

Cited by 123 publications

(90 citation statements)

References 35 publications

(30 reference statements)

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“…In spite of lack of molecular descriptions, serological evidences of infections have been reported for humans (6,8,12,22,29,42) and other animals (46). Since anti-HEV immunoglobulin G (IgG) has been detected in approximately 94% of 20-week old pigs in New Zealand (11) and these animals are thought to be a source for transmission of HEV to humans, in order to detect and characterize an HEV RNA in Brazil, we collected feces of pigs at Figure 1.…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of swine hepatitis E virus from Southeastern Brazil

Paiva

Tzaneva

Haddad

et al. 2007

Braz. J. Microbiol.

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Despite serological evidences of presence of hepatitis E virus (HEV) in humans or other animals, until this study the virus had not been detected and molecular characterization of the isolate that circulates in Brazil had not been described. Thus, we collected stool samples of young pigs and tested for presence of HEV RNA by RT-PCR, using primers for partial amplification of ORF2 sequence. Phylogenetic analysis with sequence obtained from the amplified products revealed that the HEV isolate identified here was most closely related to HEV isolates of genotype 3, which is commonly detected in HEV infected pigs. Nucleotide sequence analyses carried out with the entire amplified fragment, ORF2/ORF3 overlapping and ORF2 non-overlapping sequences showed highest identities with the US isolate of genotype 3. Similarly, amino acid sequence analyses done with the entire amplified fragment, ORF2 non-overlapping, ORF2 and ORF3 overlapping sequences also showed highest identities with the genotype 3 isolate. Presence, in Brazil, of HEV of genotype 4, which also infects pigs, as well as HEV strains that infect humans still remain to be detected and characterized.

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Section: Discussionmentioning

confidence: 99%

Molecular characterization of swine hepatitis E virus from Southeastern Brazil

Paiva

Tzaneva

Haddad

et al. 2007

Braz. J. Microbiol.

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“…However, there is an agreement among the present data and others on the concept that swine HEV causes almost universal infection in pigs at least in some regions. For instance, in the southeast from Brazil, swine IgG anti-HEV was detected in 95.5% of pigs older than 20 weeks of age (19).…”

Section: Discussionmentioning

confidence: 99%

“…Serum samples were tested for IgG anti-HEV by EIA according to the method described previously and standardized by Obriadina et al (10), modified by the use of anti-swine IgGhorseradish peroxidase enzyme conjugate (Sigma Chemicals St Louis MO), as described elsewhere (19). This EIA uses two HEV recombinant proteins, a mosaic protein (MP-II) and a protein containing region 452-617 aa of the ORF2 of the HEV Burma strain as coating antigens.…”

Section: Detection Of Igg Anti-hevmentioning

confidence: 99%

“…Data about the possibility of HEV circulation among animal species are still scarce in Brazil. Nonetheless, high anti-HEV prevalence among swine herds from the southeast of Brazil was recently reported (19). Aiming to investigate if HEV circulates among swines in Mato Grosso State, Central Brazil, we performed a cross-sectional survey in pigs from different counties of this region.…”

Section: Introductionmentioning

confidence: 99%

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Hepatitis E virus antibodies in swine herds of Mato Grosso state, Central Brazil

Guimarães¹,

Saddi²,

Vitral³

et al. 2005

Braz. J. Microbiol.

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There is no information about evidence of hepatitis E virus (HEV) infection in swines in Central Brazil. In order to assess if HEV circulates in swines of the State of Mato Grosso, Central Brazil, a seroprevalence study was conducted including pigs from 17 farms, corresponding to 13 counties. The animals were randomly chosen among pigs arriving to two slaughterhouses between December 2002 and February 2003. Serum samples were collected and tested for IgG antibodies against HEV (anti-HEV) by enzyme immunoassay (EIA). This EIA uses two HEV recombinant proteins as antigens, a mosaic protein (MP-II) and a protein containing region 452-617 of the ORF2 of the HEV Burma strain as coating antigens. 211 out of 260 pigs (81.2%; 95CI = 75.7%, 85.6%) were anti-HEV reactive. The seropositivity did not vary with gender or age, but ranges from 15% to 100% among the farms. Our results point out that HEV seems to circulate among pigs in Mato Grosso State, suggesting that this virus is spread over the region, as seen in other countries worldwide.

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“…In addition to the findings in humans, serologic evidence of infection has been found in other species such as cattle, dogs and chickens (6), and the viral genome in wild boars, deer, rabbits, rats and bats (2,7,8) but it is still unclear what role these species play in the epidemiology of the infection. On the other hand, the increased…”

Section: Introductionmentioning

confidence: 96%

Serological evidence of Hepatitis E Virus infection in Antioquia, Colombia slaughtered pigs

Gutiérrez

et al. 2015

Rev MVZ Córdoba

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Objective. To detect the presence of specific antibodies against Hepatitis E virus (HEV) in pigs slaughtered in Antioquia, the department where the greatest amount of pork is produced and consumed in Colombia. Materials and methods. Between September 2011 and May 2012, blood samples from pigs were obtained in five slaughterhouses of Antioquia, four of them located in the Aburrá Valley subregion and other located in northern subregión. Serum were evaluated with a commercial ELISA kit for diagnosing HEV in humans but adapted to detect IgG and IgM antibodies in pigs. Results. A 100.0% seropositivity for IgG antibodies was found in 1000 samples evaluated, and 82.06% for IgM antibodies were found in 740 samples. Conclusions. These results indicate that pigs in slaughter age in Antioquia, and possibly in Colombia, have been exposed to HEV at some point in their production process and a high percentage of them can arrive to slaughterhouses with recent infection.

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Serological evidence of hepatitis E virus infection in different animal species from the Southeast of Brazil

Abstract: Serological evidence of hepatitis E virus infection (HEV) has been observed in both humans

Cited by 123 publications

References 35 publications

Molecular characterization of swine hepatitis E virus from Southeastern Brazil

Molecular characterization of swine hepatitis E virus from Southeastern Brazil

Hepatitis E virus antibodies in swine herds of Mato Grosso state, Central Brazil

Serological evidence of Hepatitis E Virus infection in Antioquia, Colombia slaughtered pigs

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