2005
DOI: 10.1590/s0100-736x2005000400005 View full text |Buy / Rent full text
|
|

Abstract: A rapid indirect enzyme-linked immunosorbent assay (ELISA) was developed for measuring antibodies against Leishmania chagasi using total antigen from lysed promastigotes. Fifty symptomatic mixed breed dogs from a region of high incidence of visceral leishmaniasis in Brazil were examined. The results showed that in the positive animals, diagnosed by cytological examination, the ELISA using protein A assay system (mean optical density ± SD / 2.078 ± 0.631) detected more antibodies than the anti-IgG assay (mean o… Show more

Help me understand this report

Search citation statements

Order By: Relevance
Select...
2
2
1
0
23
0
4

Year Published

2012
2012
2015
2015

Publication Types

Select...
2
1

Relationship

0
3

Authors

Journals

0
23
0
4
Order By: Relevance
“…A maior reatividade da proteína A (média geral de DO 0,546) no ELISA indireto pode ser explicada por sua alta afinidade pelos anticorpos da classe IgG presentes no soro em resposta específica à infecção (Lima et al 2005). A Proteína A pode reagir tanto com as subclasses IgG1, IgG2a, IgG2b e IgG3, se ligando à cadeia pesada do anticorpo, quanto com os anticorpos das classes IgM e IgA caninas (Goudswaard et al 1978, Lima et al 2005.…”
Section: Discussionunclassified
“…A Proteína A pode reagir tanto com as subclasses IgG1, IgG2a, IgG2b e IgG3, se ligando à cadeia pesada do anticorpo, quanto com os anticorpos das classes IgM e IgA caninas (Goudswaard et al 1978, Lima et al 2005. Dessa forma, animais na fase aguda da infecção, ainda não detectáveis em testes de triagem com métodos baseados no uso de anti-IgG de cão doméstico, podem ser identificados no ensaio padronizado com a Proteína A, que ainda parece detectar uma maior abrangência de moléculas de IgGs.…”
Section: Discussionunclassified
See 1 more Smart Citation
“…Serum samples were selected using archived material (NUNES et al, 2008) kept at -80 °C. Serological evaluation was performed by indirect ELISA as described by Lima et al (2005) and by a quick immunochromatographic assay based on Dual-Path Platform technology (DPP®CVL rapid test, Fiocruz/Bio-Manguinhos Unit, Brazil). DNA was extracted from total blood samples using the phenol/chloroform method and PCR was performed using specific Leishmania primers for kDNA amplification (120bp), as previously described (NUNES et al, 2007).…”
Section: Sampling and Cvl Diagnosismentioning
“…ELISA (Enzyme Linked Immunosorbent Assay) represents a simple and rapid method for the survey of this canine infection (Lima et al, 2005), allowing the processing of a considerable number of samples in a short time interval (Maia & Campino, 2008).…”
Section: Diagnosismentioning