Serological detection of Brucella canis in shelter dogs from Northern Paraná

Silva, Luiz César; Leuzzi, Luis Álvaro; Nassar, Julyerme Luiz Bagatim; Junior, Flávio Antônio Barca; Headley, Selwyn Arlington; Okano, Werner; Kemper, Bernando; Traap, Sílvia Manduca

doi:10.5433/1679-0359.2012v33n6p2391

Cited by 2 publications

(1 citation statement)

References 10 publications

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“…abortus antibodies in both cities studied was observed. Similar results were observed in other regions of the country (Porto et al 2008;Zanetti et al 2011;Silva et al 2012). From the dogs used in the study 99% came from urban area and from these ones, only 0.07% had access to rural properties anytime in life, therefore, being less prone to infection by B. abortus.…”

Section: Discussionsupporting

confidence: 86%

Seroprevalence for brucellosis and leptospirosis in dogs from Belém and Castanhal, State of Pará, Brazil

et al. 2015

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Brucellosis and leptospirosis are widely spread bacterial infections and dogs are the most important source of infection and reservoir for diseases. Dogs can disseminate the agents in the environment and transmit them to humans and/or other animals. The objective of this study was assess the occurrence of reactive to antibodies anti-Leptospira spp., Brucella canis and B. abortus in Belém and Castanhal, State of Pará, Amazon, Brazil. A total of 156 samples were randomly collected in the city of Belém and 158 samples in Castanhal. The anti-B. canis antibodies research was performed by Agar Gel Immunodiffusion (AGID) with and without 2-mercaptoethanol serum treatment (AGID-2ME). To assess the anti-B. abortus antibodies, the technique of Fast Seroagglutination with buffered acidified plate antigen (BAPAT) was used. For anti-Leptospira spp. antibodies research, the Microscopic Agglutination Technique (MAT) was used. No animal reacted to Brucella abortus and one animal was reactive to B. canis at the AGID, but it was negative to the AGID-2ME test. Seventeen percent of dogs (47/274) presented anti-Leptospira spp. antibodies, with prevalence of serovar Canicola. The dogs from Belém and Castanhal are not source of infection for B. abortus and B. canis, however, they are reservoirs for different serovars of Leptospira spp. KEYWORDS: Leptospira spp., Brucella spp., domestic canids, Serology Soroprevalência para brucelose e leptospirose em cães de Belém e Castanhal, Pará, Brasil RESUMO Brucelose e leptospirose são enfermidades bacterianas amplamente disseminadas e o cão é importante fonte de infecção e reservatório para ambas, podendo eliminar o agente no meio ambiente, e transmiti-lo para humanos e/ou outros animais. O presente estudo teve como objetivo pesquisar a ocorrência de cães reagentes para anticorpos contra Leptospira spp., Brucella canis e B. abortus em Belém e Castanhal, Pará, Amazônia, Brasil. Foram colhidas de forma aleatória 156 amostras no município de Belém e 158 amostras em Castanhal. A pesquisa de anticorpos anti-

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Section: Discussionsupporting

confidence: 86%

Seroprevalence for brucellosis and leptospirosis in dogs from Belém and Castanhal, State of Pará, Brazil

et al. 2015

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Canine Brucellosis: An Update

et al. 2021

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Canine brucellosis is an infectious and zoonotic disease caused by Brucella canis, which has been reported worldwide, and is a major public health concern due to close contact between dogs and humans. In dogs, canine brucellosis manifests with abortion outbreaks, reproductive failure, enlargement of lymph nodes, and occasionally affects the osteoarticular system, although the occurrence of asymptomatic infections in dogs are not uncommon. In humans, the disease is associated with a febrile syndrome, commonly with non-specific symptoms including splenomegaly, fatigue, and weakness. Infection of dogs occurs mostly by the oronasal route when in contact with contaminated tissues such as aborted fetuses, semen, urine, and vaginal secretions. In humans, contact with contaminated fluids from infected dogs is an important source of infection, and it is an occupational risk for veterinarians, breeders, laboratory workers, among other professionals who deal with infected animals or biological samples. The diagnosis in dogs is largely based on serologic methods. However, serologic diagnosis of canine brucellosis remains very challenging due to the low accuracy of available tests. Molecular diagnostic methods have been increasingly used in the past few years. Treatment of infected dogs is associated with a high frequency of relapse, and should be employed only in selected cases. Currently there are no commercially available vaccines for prevention of canine brucellosis. Therefore, development of novel and improved diagnostic methods as well as the development of efficacious and safe vaccination protocols are needed for an effective control of canine brucellosis and its associated zoonotic risk.

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Serological detection of Brucella canis in shelter dogs from Northern Paraná

Cited by 2 publications

References 10 publications

Seroprevalence for brucellosis and leptospirosis in dogs from Belém and Castanhal, State of Pará, Brazil

Seroprevalence for brucellosis and leptospirosis in dogs from Belém and Castanhal, State of Pará, Brazil

Canine Brucellosis: An Update

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