Serologic Diagnosis of Human Taenia solium Cysticercosis by Using Recombinant and Synthetic Antigens in QuickELISA™

Abstract

Abstract. Diagnosis of Taenia solium cysticercosis is an important component in the control and elimination of cysticercosis and taeniasis. New detection assays using recombinant and synthetic antigens originating from the lentil lectinpurified glycoproteins (LLGPs) of T. solium cysticerci were developed in a QuickELISA™ format. We analyzed a panel of 474 serum samples composed of 108 serum samples from donors with two or more viable cysts, 252 serum samples from persons with other parasitic infections, and 114 serum samples from persons with no documented illnesses. The sensitivities and specificities of T24H QuickELISA™, GP50 QuickELISA™, and Ts18var1 QuickELISA™ were 96.3% and 99.2%, 93.5% and 98.6%, and 89.8% and 96.4%, respectively, for detecting cases with multiple, viable cysts. T 24H QuickELISA™ performs best among the three assays, and has sensitivity and specificity values comparable to those of the LLGP enzyme-linked immunosorbent blot. The QuickELISA™ are simple, rapid quantitative methods for detecting antibodies specific for T. solium cysticerci antigens.

Recombinant Protein- and Synthetic Peptide-Based Immunoblot Test for Diagnosis of Neurocysticercosis

“…In this study, the rT24H antigen alone had a sensitivity of 99% and a specificity of 100% for NCC cases with more than one viable cyst, which was comparable to those of the LLGP-EITB assay using native proteins. In multiple assay formats, rT24H alone has performed as well or better than the combination of antigens (16,26,28). Based on the data from this and other studies using the recombinant LLGP-derived proteins, we propose that rT24H can be used alone and should be an option for laboratories and manufacturers to consider, since its use alone may simplify the interpretation of results and reduce the cost of the assay.…”

“…In another study that evaluated the performance of several 8-kDa proteins in a multiantigen print immunoassay (MAPIA), sTSRS1 showed a sensitivity of 81% (26). Typically, members of the 8-kDa-protein family have been the least sensitive antigens when these peptides were compared to other LLGP-derived recombinant proteins, with sensitivities ranging from 77% in the MAPIA to 97% using an immunoblot test (25)(26)(27)(28)(29). It is unclear why the 8-kDa peptides performed differently in an earlier study using a similar immunoblot test (25).…”

Serodiagnosis of human neurocysticercosis using antigenic components of Taenia solium metacestodes derived from the unbound fraction from jacalin affinity chromatography

“…Moreover, the DJ unbound fraction provided superior results to those obtained by other authors using whole extracts, where the sensitivity ranged from 70-85% and the specificity ranged from 80-88% (Bueno et al 2000, Shiguekawa et al 2000 for the detection of anti-T. solium metacestodes IgG by ELISA. Although saline extracts have been widely used for primary screening in endemic areas, the identification and purification of highly specific glycoproteins associated with T. solium metacestodes have been the main focus for the serodiagnosis of NCC (Ito 2002, Lee et al 2011. It is important to make clear that the other investigators used panels of lectins in order to identify NCC antigens and the jacalin was included in those panels (Trindade et al 2006, Zhang et al 2006.…”

“…For these reasons, immunoassays that are standardised for a proper diagnosis of NCC will lead to better clinical management of NCC patients because the immunoassays support diagnoses for patients whose clinical and imaging profiles are compatible with NCC (Michelet et al 2011). Among various immunological methods, the ELISA and immunoblot are the most frequently used to detect antibodies against T. solium antigens in serum or cerebrospinal fluid samples, but these tests have shown different degrees of sensitivity and specificity, depending on the method of antigen preparation used (Shiguekawa et al 2000, Barcelos et al 2007, Deckers & Dorny 2010, Gonçalves et al 2010, Lee et al 2011, Michelet et al 2011, Ferrer et al 2012). …”

Taenia solium Taeniasis and Cysticercosis in Southeast Asia

“…Newer QuickELISA™ (Immunetics Inc., Boston, MA) assays also look promising due to the ease of use, but their performance has not been widely validated in various field conditions including SEA. 51 In addition, diagnostic tools such as coprology, coproantigen ELISA, and molecular methods (such as polymerase chain reactions) vary widely in their performance in identification of taeniasis cases (with or without accompanying cysticercosis). 52 Coprology methods struggle with sensitivity and species identification; this is especially problematic in SEA where sympatric infections with other Taenia species are common.…”