Sericin silk protein in peripheral nervous repair associated with the physical exercise of swimming in Wistar rats

Debastiani, Jean Carlos; Santana, André Junior; Ribeiro, Lucinéia de Fátima Chasko; Brancalhão, Rose Meire Costa; Bertolini, Gladson Ricardo Flor

doi:10.1080/01616412.2018.1564187

Cited by 8 publications

(4 citation statements)

References 25 publications

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“…Despite the negative findings for the exercise protocol proposed, good evolution of the nociceptive threshold and allodynia and for the nerve fiber variables had been shown after swim exercise with overload in sciatic nerve crush model, however the animals swam for 15, 20, and 25 min with an overload of 10% of the BW during 3 weeks (Debastiani et al, 2019), while in the current study the animals swam for an hour with a shorter protocol of 2 weeks. De Moraes et al (2018) used the swimming as a therapeutic alternative for sciatic nerve regeneration.…”

Section: Discussioncontrasting

confidence: 67%

Negative neuromuscular and functional repercussion of forced swimming after axonotmesis

Moura¹,

Morais²,

Barbosa³

et al. 2022

J Exerc Rehabil

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Peripheral nerve injuries are cause of sensory disturbances and in functional abilities, and are associated personal and social costs. Strat-egies that maximize nerve regeneration and functional recovery are necessary, the exercise is an option. This study evaluated the effects of forced swimming exercise on neuromuscular histomorphometry and on functional recovery in a median nerve crush model. Sixteen Wistar rats underwent median nerve crush and were divided into control group (CG) and swimming group (SG). The forced swimming protocol started one week after the injury and was performed for 1 hr a day, 5 days per week, for 2 weeks. The rats swam with an overload of 5% and 10% of body weight in the first and second week, respectively. The functional recovery was assessed in three moments using the grasping test. On day 21, fragments of the median nerve and of the forearm flexors mus-cles were removed for histomorphometric analysis. The SG had func-tional recovery impaired (p<0.001) and presented lower myelinated fi-bers number, fiber and axon minimal diameter, myelin thickness and g-ratio in the proximal e distal segments of the median nerve (p<0.005) and area muscle fiber (p<0.005) than CG. Also, the SG presented a number of capillaries in the proximal segments of the median nerve greater than CG (p<0.005). The exercise protocol used in this study im-paired the regeneration of the median nerve and negatively influenced the functional recovery.

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Section: Discussioncontrasting

confidence: 67%

Negative neuromuscular and functional repercussion of forced swimming after axonotmesis

Moura¹,

Morais²,

Barbosa³

et al. 2022

J Exerc Rehabil

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“…Moreover, in an in vitro model of traumatic optic neuropathy, electrospun silk fibers loaded with growth factor were reported to promote survival and neurite outgrowth of the retinal ganglion cells (Wittmer et al, 2011). A recent in vivo study has also shown that topical sericin combined with swimming exercise improved sciatic nerve injury in Wistar rats (Debastiani et al, 2019).…”

Section: Neuroregenerationmentioning

confidence: 99%

Identification and applications of neuroactive silk proteins: a narrative review

Mohammadi¹,

Sadigh‐Eteghad²,

Torbati³

et al. 2019

JAB

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In traditional medicine, natural silk is regarded as a cognitive enhancer and a cure for ameliorating the symptoms of heart disease, atherosclerosis, and metabolic disorders. In this review, general characteristics of both silk proteins, fibroin and sericin, extracted from silkworm Bombyx mori and their potential use in the neuronal disorders was discussed. Evidence shows that silk proteins exhibit neuroprotective effects in models of neurotoxicity. The antioxidant, neuroprotective, and acetylcholinesterase inhibitory mechanisms of silk proteins could prove promising in the treatment of neurodegenerative diseases. Owing to their excellent neurocompatibility and physicochemical properties, silk proteins have been used as scaffolds and drug delivery materials in the neuronal tissue engineering. These data support the potential of silk proteins as an effective complementary agent for central and peripheral neurological disorders.

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“…However, because sericin has been shown to elicit the inflammatory response in vivo, complete removal of sericin, also known as degumming, is required before it can be used ( 32 ). However, purified sericin has been shown to influence cartilaginous tissue regeneration, promoting cell regeneration, skin tissue regeneration, and the healing process with low levels of the inflammatory response ( 33 ). Due to its outstanding biocompatibility and tunable degradation qualities, silk fibroin is a promising biomaterial for tissue engineering and regenerative medicine.…”

Section: Introductionmentioning

confidence: 99%

Evaluation and Comparison of the Effects of Mature Silkworm (Bombyx mori) and Silkworm Pupae Extracts on Schwann Cell Proliferation and Axon Growth: An In Vitro Study

et al. 2023

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Background: Silkworm products were first used by physicians more than 8500 years ago, in the early Neolithic period. In Persian medicine, silkworm extract has several uses for treating and preventing neurological, cardiac, and liver diseases. Mature silkworms (Bombyx mori) and their pupae contain a variety of growth factors and proteins that can be used in many repair processes, including nerve regeneration. Objectives: The study aimed to evaluate the effects of mature silkworm (Bombyx mori), and silkworm pupae extract on Schwann cell proliferation and axon growth. Methods: Silkworm (Bombyx mori) and silkworm pupae extracts were prepared. Then, the concentration and type of amino acids and proteins in the extracts were evaluated by Bradford assay, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and liquid chromatograph-mass spectrometer (LC-MS/MS). Also, the regenerative potential of extracts for improving Schwann cell proliferation and axon growth was examined by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay, electron microscopy, and NeuroFilament-200 (NF-200) immunostaining. Results: According to the results of the Bradford test, the total protein content of pupae extract was almost twice that of mature worm extract. Also, SDS-PAGE analysis revealed numerous proteins and growth factors, such as bombyrin and laminin, in extracts that are involved in the repair of the nervous system. In accordance with Bradford's results, the evaluation of extracts using LC-MS/MS revealed that the number of amino acids in pupae extract was higher than in mature silkworm extract. It was found that the proliferation of Schwann cells at a concentration of 0.25 mg/mL in both extracts was higher than the concentrations of 0.01 and 0.05 mg/mL. When using both extracts on dorsal root ganglion (DRGs), an increase in length and number was observed in axons. Conclusions: The findings of this study demonstrated that extracts obtained from silkworms, especially pupae, can play an effective role in Schwann cell proliferation and axonal growth, which can be strong evidence for nerve regeneration, and, consequently, repairing peripheral nerve damage.

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Sericin silk protein in peripheral nervous repair associated with the physical exercise of swimming in Wistar rats

Cited by 8 publications

References 25 publications

Negative neuromuscular and functional repercussion of forced swimming after axonotmesis

Negative neuromuscular and functional repercussion of forced swimming after axonotmesis

Identification and applications of neuroactive silk proteins: a narrative review

Evaluation and Comparison of the Effects of Mature Silkworm (Bombyx mori) and Silkworm Pupae Extracts on Schwann Cell Proliferation and Axon Growth: An In Vitro Study

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