2013
DOI: 10.1128/jcm.02927-12
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Abstract: cWe developed and validated a real-time PCR assay consisting of 7 triplexed reactions to identify 11 individual serotypes plus 10 small serogroups representing the majority of disease-causing isolates of Streptococcus pneumoniae. This assay targets the 13 serotypes included within the 13-valent conjugate vaccine and 8 additional key serotypes or serogroups. Advantages over other serotyping assays are described. The assay will be expanded to 40 serotypes/serogroups. We will provide periodic updates at our proto… Show more

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Cited by 125 publications
(123 citation statements)
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“…However, with the developments of vaccination and the resulting serotype shift, serotyping of pneumococci has returned to the forefront of clinical interest. As long as pneumococcal vaccines are based on PS capsules, there will be a need for methods to determine capsular types (202)(203)(204)(205). A serotyping system should ideally detect all the known pneumococcal serotypes and then distinguish each serotype; however, in view of potentially vast capsular diversity, one needs to consider how much resolution a serotyping system needs and adjust analytical demands accordingly.…”
Section: Analytical Approaches To Serotyping Pneumococcimentioning
confidence: 99%
“…However, with the developments of vaccination and the resulting serotype shift, serotyping of pneumococci has returned to the forefront of clinical interest. As long as pneumococcal vaccines are based on PS capsules, there will be a need for methods to determine capsular types (202)(203)(204)(205). A serotyping system should ideally detect all the known pneumococcal serotypes and then distinguish each serotype; however, in view of potentially vast capsular diversity, one needs to consider how much resolution a serotyping system needs and adjust analytical demands accordingly.…”
Section: Analytical Approaches To Serotyping Pneumococcimentioning
confidence: 99%
“…Samples should have sufficient pneumococcal loads, and serotype-specific analysis of the data will be limited. With ongoing advances in molecular methods, including increased sensitivity and the ability to detect more individual serotypes (17), many of these limitations will likely be overcome. However, the large number of pneumococcal serotypes and Other refers to the remaining serotypes included in the PCR assay, and NEG42 refers to the samples negative for the 42 serotypes detected by the assay.…”
Section: Discussionmentioning
confidence: 99%
“…For decades, the Quellung reaction (12) has been the gold standard for serotyping; however, the method is culture dependent and therefore is not useful for culture-negative lytA-positive specimens. Sequencing of the capsular polysaccharide synthesis genes (13,14) has enabled the development of conventional (15) and real-time PCR (16)(17)(18) assays. The increased sensitivity of real-time PCR assays compared to that of conventional PCR assays increases the potential to serotype specimens with low bacterial loads.…”
mentioning
confidence: 99%
“…[16][17][18][19][20][21][22][23][24][25][26][27][28] Since cmPCR have a limited number of serotypes in each PCR reaction, they have been designed to target the most prevalent serotypes causing IPD, often in sequential reactions. [21][22][23][24][25][26] However, sequential PCR may not be the most cost effective strategy to identify S. pneumo-niae serotypes that are vaccine-preventable. This study used oligonucleotide permutations in a modified set of cmPCR reactions (termed cmPCRmod) to reduce the amount of testing required to identify S. pneumoniae serotypes covered by the PCV7, PCV13, or PPV23 vaccines.…”
Section: Introductionmentioning
confidence: 99%