Sequential hydrolysis of waste newspaper and bioethanol production from the hydrolysate

Wu, Fang-Chen; Huang, Shu-Sing; Shih, Ing-Lung

doi:10.1016/j.biortech.2014.06.041

Cited by 24 publications

(10 citation statements)

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“…The hydrolysis of pretreated NW obtained by using mixes of purified enzymes showed that the EA method improve the saccharification in comparison with the AFEX, although the hydrolysis yields remained low in comparison to the data previously reported in literature. The maximum total polysaccharides conversion yield (for EA-pretreated-NW hydrolyzed by enzymatic MIX A) was similar to the yields reported by Ali and Khan Mohd ( 2011 ) after basic pretreatment and six days of microbial hydrolysis by Aspergillus niger and lower than the most recent reported results (Wua et al 2014 ; Chu and Feng 2013 ; Wang et al 2012b ; Guerfali et al 2015 ; Subhedar and Gogate 2014 ; Kim et al 2006 ; Xin et al 2010 ). Moreover, the glucan conversion yield was very low (not exceeding 7.3 %).…”

Section: Discussionsupporting

confidence: 88%

“…Furthermore, composition analysis of EA pretreated NW revealed that this technique, similarly to the AFEX pretreatment, does not significantly change the composition. Furthermore, the cellulose percentage after acid pretreatment, reported by Guerfali et al ( 2015 ), and the holocellulose percentage, after alkaline pretreatment, reported by Wu et al ( 2014 ), were comparable to data obtained after AFEX or EA method; contrariwise, the alpha cellulose and holocellulose percentage, reported by Sangkharak ( 2011 ), after pretreatment by using NaOH are both higher than those reported in this study, but the reaction conditions used for AFEX reaction are milder (65 versus 100 °C and residence time 15 min versus 4 h for AFEX and basic pretreatment respectively).…”

Section: Discussionmentioning

confidence: 92%

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Saccharification of newspaper waste after ammonia fiber expansion or extractive ammonia

et al. 2016

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The lignocellulosic fractions of municipal solid waste (MSW) can be used as renewable resources due to the widespread availability, predictable and low pricing and suitability for most conversion technologies. In particular, after the typical paper recycling loop, the newspaper waste (NW) could be further valorized as feedstock in biorefinering industry since it still contains up to 70 % polysaccharides. In this study, two different physicochemical methods—ammonia fiber expansion (AFEX) and extractive ammonia (EA) were tested for the pretraetment of NW. Furthermore, based on the previously demonstrated ability of the recombinant enzymes endocellulase rCelStrep, α-l-arabinofuranosidase rPoAbf and its evolved variant rPoAbf F435Y/Y446F to improve the saccharification of different lignocellulosic pretreated biomasses (such as corn stover and Arundo donax), in this study these enzymes were tested for the hydrolysis of pretreated NW, with the aim of valorizing the lignocellulosic fractions of the MSW. In particular, a mixture of purified enzymes containing cellulases, xylanases and accessory hemicellulases, was chosen as reference mix and rCelStrep and rPoAbf or its variant were replaced to EGI and Larb. The results showed that these enzymatic mixes are not suitable for the hydrolysis of NW after AFEX or EA pretreatment. On the other hand, when the enzymes rCelStrep, rPoAbf and rPoAbf F435Y/Y446F were tested for their effect in hydrolysis of pretreated NW by addition to a commercial enzyme mixture, it was shown that the total polysaccharides conversion yield reached 37.32 % for AFEX pretreated NW by adding rPoAbf to the mix whilst the maximum sugars conversion yield for EA pretreated NW was achieved 40.80 % by adding rCelStrep. The maximum glucan conversion yield obtained (45.61 % for EA pretreated NW by adding rCelStrep to the commercial mix) is higher than or comparable to those reported in recent manuscripts adopting hydrolysis conditions similar to those used in this study.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 92%

Saccharification of newspaper waste after ammonia fiber expansion or extractive ammonia

et al. 2016

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“…The high lignin content means more mechanical pulp fiber. The lignin composition was close to that of native softwood, 25 to 35% (Wu et al 2014). The remainder of the pulp was made up of hemicelluloses and ash.…”

Section: Compositional Analysis Of Fraction Pulp From Waste Papermentioning

confidence: 86%

Comparative Study of Enzymatic Hydrolysis Properties of Pulp Fractions from Waste Paper

Li¹,

Yu²,

Sun³

et al. 2015

BioResources

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As a lignocellulosic material, wastepaper is a potential material for ethanol production. However, little research on the enzymatic hydrolysis of wastepaper pulp has been conducted. In this study, the enzymatic hydrolysis of different waste pulp fractions (R80 represents greater than 80-mesh wastepaper pulp, R80-180 represents the range of 80-to 180-mesh wastepaper pulp, and R180 represents smaller than 180-mesh waste paper pulp) were carried out at 50 °C, pH 4.8, for 96 h, with a substrate concentration of 5% (w/v) and cellulase loading of 18 FPU/g cellulose. In terms of the specific surface area, fiber structure, and surface morphology, R80-180 had the highest affinity to cellulase and therefore the highest glucose yield of 80.33%. R180 had the lowest glucose yield (55.36%) because of its high ash content (21.36%), which reduced the adsorption of cellulase to cellulose. The enzymatic hydrolysis of R80 mixed with R80 or R80-180 was also studied. Results indicated that adding R80-180 increased the glucose yield of R80. The highest glucose yield (82.57%) was obtained when 15% R80-180 was mixed with R80. However, the glucose content decreased when R180 was mixed with R80 because of its high ash content.

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“…Entre os materiais lignocelulósicos estão, por exemplo, palha, serragem, bagaço, ripas de madeira, gramas, resíduos da atividade ou culturas agrícolas, RSD e resíduos de papel (BRUMMER et al, 2014;WU;HUANG, SHIH, 2014).…”

Section: Figura 31 -Fluxograma De Reações Metabólicas Da Fermentaçãounclassified

“…O potencial de produção de energia na forma de hidrogênio, etanol e metano a partir de papel por meio de uma variedade de processos tem sido avaliado por muitos pesquisadores (BOTTA, 2012;CHAIRATTAMANOKORN et al, 2012;ELLISTON et al, 2013;WU;HUANG, SHIH, 2014;YEN, BRUNE, 2007). Entretanto, apesar de ser comprovada a possibilidade de geração de vetores energéticos a partir do papel, a hidrólise desta matéria-prima a açúcares fermentáveis ainda deve ser avaliada, levandose em conta, principalmente, a redução do custo e do potencial poluidor dos prétratamentos de hidrólise aplicados (hidrólise ácida e enzimática, por exemplo).…”

Section: Introductionunclassified

Hidrólise e fermentação de papel em lisímetro para recuperação de compostos de interesse biotecnológico

Botta¹

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This study evaluated the production of compounds of biotechnological interest and potential energy vectors from office paper in lysimeters (20L), using a microbial consortium purified from rumen fluid. A central composite design (CCD) was performed to verify the influence of three independent variables on paper conversion to hydrogen and other organic compounds in bench lysimeter. The tested variables were: mass of paper (x1: 500g, 750g and 1000g), moisture content (x2: 50%, 65% and 80%), and incubation temperature (x3: 35 o C, 45 o C and 55 o C). The dependent variables of CCD were production of hydrogen (Y1: mmol), acetic acid (Y2: mg/L), ethanol (Y3:mg/L) and metanol (Y4:mg/L). For monitoring the lysimeters in relation to paper hydrolysis and fermentation, analyses of biogas (H2, N2, CO2 and CH4) and the organic compounds'concentrations in the leachate, such as, total sugars, chemical oxygem demand, volatile organic acids (VOA's) and alcohols were conducted during operation. Alcalinity, pH and total solids content of the leachate were also monitored. Massive sequencing of rRNA 16S (Illumina) was carried out for identification of the microorganisms of the in natura rumen fluid, the purified consortium, and those collected from lysimeters R2, R5 and R9. Hydrogen production was detected only in lysimeters R1 (25 mmol), R2 (35 mmol) and R5 (3 mmol), all of them operated with 80% of moisture content. In R1 and R2, high concentrations of acetic acid, of 21.500 and 17.000, respectively, were due to the likely occurrence of homoacetogenesis. Under thermophilic temperature, especially R5, the hydrogen production was detected in low quantity, and the highlight was the production of ethanol and methanol, with concentrations around 2.300 and 5.600 mg/L, respectively. At 80% moisture condition (R1, R2, R5, R6), high percentages of paper removal and sharp fermentative activity were observed. However, at lower moisture conditions, the microbial growth was unfavored, independent of the temperature. Low paper consumption and reduced concentrations of OVA's and alcohols were detected in R3, R4, R7 and R8, all of them operated with 50% of moisture content. In R9 and R10, operated at 45 o C and 65% of moisture, there was also attenuated production of VOA's and alcohols, with absence of hydrogen. According to CCD statistical analysis, paper moisture content had positive effect statistically significative on hydrogen, acetic acid and etanol production. The temperature had positive effect on hydrogen and acetic acid production. And the mass of paper dit not have effect statistically significative for any dependent variables. The most abundant bacterial genus was: Prevotella in the in natura rumen fluid (F.N.) Dysgonomonas in the purified consortium and R2 (35 o C), Thermicanus in R5 (55 o C) and Phaeospirillum in R9 (45 o C). In conclusion, moisture content was the main parameter to promote paper hydrolysis and fermentation; temperature was the principal variable that influenced the structure of microbial community, confirmed by the...

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Sequential hydrolysis of waste newspaper and bioethanol production from the hydrolysate

Cited by 24 publications

References 29 publications

Saccharification of newspaper waste after ammonia fiber expansion or extractive ammonia

Saccharification of newspaper waste after ammonia fiber expansion or extractive ammonia

Comparative Study of Enzymatic Hydrolysis Properties of Pulp Fractions from Waste Paper

Hidrólise e fermentação de papel em lisímetro para recuperação de compostos de interesse biotecnológico

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