Sequential cocaine-alcohol self-administration produces adaptations in rat nucleus accumbens core glutamate homeostasis that are distinct from those produced by cocaine self-administration alone

Stennett, Bethany; Padovan‐Hernandez, Yasmin; Knackstedt, Lori A.

doi:10.1038/s41386-019-0452-2

Cited by 31 publications

(64 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Following injection in the dorsal medial region of the NA core where microdialysis probe placement occurred in Experiment 1 (Fig. 2D and 5A), and our previous publications (Trantham-Davidson et al 2012;Stennett et al 2019), CTb was observed in the IL and PL, BLA, PVN, and VTA ( Fig. 5B-C).…”

Section: Methodssupporting

confidence: 73%

“…No such relationship was found for the NA core, NA shell, IL or PVN. A control group not receiving cocaine was not used for this study, as we have reported minimal (<5) Fos+ cells per region in this condition (Stennett et al 2019).…”

Section: Methodsmentioning

confidence: 99%

“…The other end exited from an incision on the rats' back and was attached to a harness (Instech, Plymouth Meeting, PA) containing a cannula (Plastics One, Roanoake, VA) that could be connected to tubing for intravenous (IV) drug delivery. For Experiment 1, immediately after catheter implantation, unilateral microdialysis guide cannula were implanted above the NA core (AP +1.2 mm, ML +2.5 mm, DV -5.0 mm) as in our previous work on this topic (Trantham-Davidson et al 2012;Stennett et al 2019). In Experiment 2, rats instead received intra-NA core Alexa Fluor™ 488 conjugated CTb (Thermo Fisher Cat.…”

Section: Surgeriesmentioning

confidence: 99%

“…Rats were then re-exposed to the context for a relapse test (100 min) during which time presses on the active lever yielded the drug-associated cues but no drug. The length of the test was chosen based on our recent assessment of the same dependent variables (Fos and glutamate efflux) with a similar timed test (Stennett et al 2019). See Fig.…”

Section: Cocaine Self-administration and Relapse Test (Experiments 1 Amentioning

confidence: 99%

“…Using the extinction-reinstatement model, glutamate efflux increases in the NA core during cocaine-, cue-, and cocaine+cue-primed reinstatement of cocaine seeking (McFarland, Lapish & Kalivas 2003;Trantham-Davidson et al 2012;Smith et al 2017;Logan, LaCrosse & Knackstedt 2018) (Stennett, Padovan-Hernandez & Knackstedt 2019). This release is necessary for reinstatement as antagonism of post-synaptic glutamate receptors and stimulation of pre-synaptic autoreceptors prevents the reinstatement of cocaine-seeking (Scofield et al 2016).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Role of prefrontal cortex projections to the nucleus accumbens core in mediating the effects of ceftriaxone on cued cocaine relapse

Bechard

Logan

Mesa

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Ceftriaxone is an antibiotic that reliably attenuates the reinstatement of cocaine-seeking after extinction while preventing the nucleus accumbens (NA) core glutamate efflux that drives reinstatement. However, when rats undergo abstinence without extinction, ceftriaxone attenuates context-primed relapse but NA core glutamate efflux still increases. Here we sought to determine if the same would occur when relapse is prompted by both context and discrete cues (context+cues) after cocaine abstinence. Male rats self-administered intravenous cocaine for 2 hr/day for 2 weeks. Cocaine delivery was accompanied by drug-associated cues (light+tone).Rats were then placed into abstinence with daily handling but no extinction training for two weeks.Ceftriaxone (200 mg/kg IP) or vehicle was administered during the last 6 days of abstinence.During a context+cue relapse test, microdialysis procedures were conducted. Rats were perfused at the end of the test for later Fos analysis. A separate cohort of rats was infused with the retrograde tracer cholera toxin B in the NA core and underwent the same self-administration and relapse procedures. Ceftriaxone increased baseline glutamate and attenuated both context+cueprimed relapse and NA core glutamate efflux during this test. Ceftriaxone reduced Fos expression in regions sending projections to the NA core (prefrontal cortex, basolateral amygdala, ventral tegmental area) and specifically reduced Fos in prelimbic cortex and not infralimbic cortex neurons projecting to the NA core. Thus, when relapse is primed by drug-associated cues and context, ceftriaxone is able to attenuate relapse by preventing NA core glutamate efflux, likely through reducing activity in prelimbic NA core-projecting neurons.

show abstract

Section: Methodssupporting

confidence: 73%

Section: Methodsmentioning

confidence: 99%

Section: Surgeriesmentioning

confidence: 99%

Section: Cocaine Self-administration and Relapse Test (Experiments 1 Amentioning

confidence: 99%