Sequence variations in the Plasmodium vivax dihydrofolate reductase-thymidylate synthase gene and their relationship with pyrimethamine resistance

Pécoulas, Philippe Eldin de; Tahar, Rachida; Ouatas, Taoufik; Mazabraud, A; Basco, Léonardo K.

doi:10.1016/s0166-6851(97)00247-8

Cited by 104 publications

(100 citation statements)

References 33 publications

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“…17,28,36 Size polymorphism in this region is resulted from variation in number of copies of the 18-basepair repeat, which is not essential for substrate binding. 21 Most (77.0%) of the isolates sequenced contain three copies of the repeat, and 23.0% contained two 18-basepair repeats.…”

Section: Resultsmentioning

confidence: 99%

Different Allele Prevalence in the Dihydrofolate Reductase and Dihydropteroate Synthase Genes in Plasmodium vivax Populations from China

Miao

Zhou

Cui

et al. 2010

The American Society of Tropical Medicine and Hygiene

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Abstract. Antifolate resistance in Plasmodium vivax is caused by point mutations in genes encoding dihydrofolate reductase ( pvdhfr ) and dihydropteroate synthase ( pvdhps ). In this study, we used direct sequencing to survey pvdhfr and pvdhps mutations in 122 clinical P. vivax isolates from a central and a southern province of China. For pvdhfr , 36.9% were wild-type, whereas mutations were detected at four codons (57, 58, 61, and 117). The S117N/T mutation was the most prevalent (48.4%), followed by the T61M mutation (18.9%). Six pvdhfr mutant alleles were found, ranging from 37.7% to 0.8%. The dramatically different pvdhfr allele frequencies between the two P. vivax populations might be caused by different drug histories or intrinsic difference between temperate and subtropical strains. In contrast, except polymorphisms within a repeat region, no resistance-conferring mutations were detected in pvdhps . Our result suggests that P. vivax populations in China may be relatively susceptible to sulfadoxine-pyrimethamine.

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Section: Resultsmentioning

confidence: 99%

Different Allele Prevalence in the Dihydrofolate Reductase and Dihydropteroate Synthase Genes in Plasmodium vivax Populations from China

Miao

Zhou

Cui

et al. 2010

The American Society of Tropical Medicine and Hygiene

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“…32 including French Guyana, Surinam, Burma, Cambodia, Indonesia, Madagascar, and Comoros Islands); and Pvs25 (10 isolates from Colombia, Honduras, Venezuela, Nicaragua, Brazil, Indonesia, North Korea, Mauritania, and Papua New Guinea). The sampling covers the distribution of P. vivax including alleles from New World and Old World isolates (22).…”

Section: Methodsmentioning

confidence: 99%

A monkey's tale: The origin of Plasmodium vivax as a human malaria parasite

Escalante

Cornejo

Freeland

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

196

210

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The high prevalence of Duffy negativity (lack of the Duffy blood group antigen) among human populations in sub-Saharan Africa has been used to argue that Plasmodium vivax originated on that continent. Here, we investigate the phylogenetic relationships among 10 species of Plasmodium that infect primates by using three genes, two nuclear (␤-tubulin and cell division cycle 2) and a gene from the plastid genome (the elongation factor Tu). We find compelling evidence that P. vivax is derived from a species that inhabited macaques in Southeast Asia. Specifically, those phylogenies that include P. vivax as an ancient lineage from which all of the macaque parasites could originate are significantly less likely to explain the data. We estimate the time to the most recent common ancestor at four neutral gene loci from Asian and South American isolates (a minimum sample of seven isolates per locus). Our analysis estimates that the extant populations of P. vivax originated between 45,680 and 81,607 years ago. The phylogeny and the estimated time frame for the origination of current P. vivax populations are consistent with an ''out of Asia'' origin for P. vivax as hominoid parasite. The current debate regarding how the Duffy negative trait became fixed in Africa needs to be revisited, taking into account not only human genetic data but also the genetic diversity observed in the extant P. vivax populations and the phylogeny of the genus Plasmodium.Duffy ͉ genetic diversity ͉ host-switch

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“…Although chloroquine (CQ) still constitutes the first-line therapy against vivax malaria, CQ resistance has been reported in both Asia and South America (Rieckman, 1989;Baird et al, 1991;Malar-Than et al, 1995;Phillips et al, 1996). In addition, resistance to the antifolate drug pyrimethamine is also widespread (de Pecoulas et al, 1998;Imwong et al, 2003). Therefore, global resurgence of vivax malaria and escalating drug-resistance demand more extensive research and control efforts.…”

Section: Introductionmentioning

confidence: 99%

Genetic structures of geographically distinct Plasmodium vivax populations assessed by PCR/RFLP analysis of the merozoite surface protein 3β gene

et al. 2006

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The recent resurgence of Plasmodium vivax malaria requires close epidemiological surveillance and monitoring of the circulating parasite populations. In this study, we developed a combination of polymerase chain reaction and restriction fragment length polymorphism (PCR/RFLP) method to investigate the genetic diversity of the P. vivax merozoite surface protein 3β (PvMSP3β) gene among four Asian parasite populations representing both tropical and temperate strains with dramatic divergent relapse patterns (N = 143). Using P. vivax field isolates from symptomatic patients, we have validated the feasibility of this protocol in distinguishing parasite genotypes. We have shown that PCR alone could detect three major size polymorphisms of the PvMSP3β gene, and restriction analysis detected a total of 12 alleles within these Asian samples. Samples from different geographical areas differed dramatically in their PvMSP3β allele composition and frequency, indicating that complex, yet different parasite genotypes were circulating in different endemic areas. This protocol allowed easy detections of multiple infections, which reached 20.5% in the samples from Thailand. It is interesting to note that samples from one temperate site in China collected during a recent outbreak of the disease also showed a high level of genetic diversity with multiple infections accounting for 5.6% of the samples. When combined with the PvMSP3α locus, this method provides better capability in distinguishing P. vivax genotypes and detecting mixed genotype infections.

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Sequence variations in the Plasmodium vivax dihydrofolate reductase-thymidylate synthase gene and their relationship with pyrimethamine resistance

Cited by 104 publications

References 33 publications

Different Allele Prevalence in the Dihydrofolate Reductase and Dihydropteroate Synthase Genes in Plasmodium vivax Populations from China

Different Allele Prevalence in the Dihydrofolate Reductase and Dihydropteroate Synthase Genes in Plasmodium vivax Populations from China

A monkey's tale: The origin of Plasmodium vivax as a human malaria parasite

Genetic structures of geographically distinct Plasmodium vivax populations assessed by PCR/RFLP analysis of the merozoite surface protein 3β gene

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