1999
DOI: 10.1292/jvms.61.429
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Abstract: ABSTRACT. The variable region in the VP2 gene of twenty-three infectious bursal disease virus (IBDV) isolates, collected in Vietnam in 1997 and 1998, was amplified as cDNA by using the reverse transcription-polymerase chain reaction and sequenced. Analysis of amino acid substitutions and phylogenetic relationships of the deduced amino acid sequences (residues 206-350)

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Cited by 9 publications
(8 citation statements)
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“…The nt sequence of the 99323 isolate was mostly similar with that of reference vvIBDV strain 89163 (only nine nt positions differed, 98.0% nt identity). Four of these nt changes were silent mutations, hence the deduced aa sequence was also very similar to that of 89163 (Figure 1) and presented the four aa positions 222A, 256I, 284I and 299S, which have been found in every vvIBDV so far characterized, with the two exceptions of an early IBDV strain from Ivory Coast that has not been reisolated since 1988 (Brown et al, 1994;Cao et al, 1998;To et al, 1999;Zierenberg et al, 2000) and of Indonesian strain Tasik94, which differs from typical vvIBDVs by lacking residue 222A (Rudd et al, 2002). However, the 99323 isolate also exhibited three non-silent nt changes, which encoded three aa changes that have not so far been found in any other vvIBDV-like viruses; namely, Y220 0/ F, G254 0/ S and A321 0/ T. These changes occurred in regions that are known to be important for antigenicity: VP2 major hydrophilic peak A (position 220), VP2 first minor hydrophilic peak (position 254) and VP2 second major hydrophilic peak (position 321) (Schnitzler et al, 1993;Vakharia et al, 1994;van den Berg et al, 1996).…”
Section: Resultsmentioning
confidence: 84%
“…The nt sequence of the 99323 isolate was mostly similar with that of reference vvIBDV strain 89163 (only nine nt positions differed, 98.0% nt identity). Four of these nt changes were silent mutations, hence the deduced aa sequence was also very similar to that of 89163 (Figure 1) and presented the four aa positions 222A, 256I, 284I and 299S, which have been found in every vvIBDV so far characterized, with the two exceptions of an early IBDV strain from Ivory Coast that has not been reisolated since 1988 (Brown et al, 1994;Cao et al, 1998;To et al, 1999;Zierenberg et al, 2000) and of Indonesian strain Tasik94, which differs from typical vvIBDVs by lacking residue 222A (Rudd et al, 2002). However, the 99323 isolate also exhibited three non-silent nt changes, which encoded three aa changes that have not so far been found in any other vvIBDV-like viruses; namely, Y220 0/ F, G254 0/ S and A321 0/ T. These changes occurred in regions that are known to be important for antigenicity: VP2 major hydrophilic peak A (position 220), VP2 first minor hydrophilic peak (position 254) and VP2 second major hydrophilic peak (position 321) (Schnitzler et al, 1993;Vakharia et al, 1994;van den Berg et al, 1996).…”
Section: Resultsmentioning
confidence: 84%
“…2). These A1a-classical virulent viruses were continually detected in the eld isolates (2001-2021), all indicated their origination of the 2512 Winter eld vaccine deravatives which have been used for vaccination since the 1990s [24,25]. Classically virulent (genogroups A1aB1 and A1aB3) and classically attenuated (genogroup A1bBx) strains were also identi ed in recent Vietnamese eld isolates (2018-2021), indicating co-existence with A3B(1/3) and demonstrating the complicated reassortment between A-genotypes (A1 and A3) and B-genotypes (B1 and B3) in circulating IBDVs in Vietnam.…”
Section: Discussionmentioning
confidence: 94%
“…GenBank: FJ842491 for segment A) in 1987 in Hanoi (Table 1) [25]. In an investigation by To et al [24], 19 "very virulent" (A3) and four "classically virulent" (A1) isolates were identi ed in the different outbreaks in Hanoi and Ho Chi Minh City between 1997 and 1998. All the ndings in the present and previous studies have implied the predominance of the A3-genotype strains over the A1-genotype strains from 1987 to 2021.…”
Section: Discussionmentioning
confidence: 99%
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