Sequence analysis of cox1 and nad1 genes in Echinococcus granulosus G3 genotype in camels (Camelus dromedarius) from central Iran

Sharbatkhori, Mitra; Harandi, Majid Fasihi; Mirhendi, Hossein; Hajialilo, Elham; Kia, Eshrat Beigom

doi:10.1007/s00436-010-2092-7

Cited by 65 publications

(53 citation statements)

References 39 publications

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“…Sharbatkhori and others 30 found that all 34 sheep isolates investigated from Tehran Province indicated the G1 genotype, in concordance with the findings of the highest prevalence of G1 genotype in humans in this study.…”

Section: Discussionsupporting

confidence: 92%

“…[48][49][50][51][52][53][54][55] For the first time in Iran, Sharbatkhori and others 30 reported the occurrence of the G3 genotype in camels from the Isfahan Province (central Iran). This was a global first for the identification of the G3 genotype in this host.…”

Section: Discussionmentioning

confidence: 99%

“…1,26 Several molecular epidemiological studies have been performed on E. granulosus isolates in Iran using sequence data of mitochondrial and nuclear genes. Overall, four different genotypes of E. granulosus (genotype G1, G3, and G6) have been reported from different livestock [27][28][29][30][31][32][33][34][35][36] and dogs (genotype G1, G2, and G3) 37 from Iran. To date, only a few human isolates of E. granulosus have been genetically characterized in Iran that indicated G1, G3, and G6 genotypes ( Table 1).…”

Section: Introductionmentioning

confidence: 99%

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Genetic Characterization of Echinococcus granulosus from a Large Number of Formalin-Fixed, Paraffin-Embedded Tissue Samples of Human Isolates in Iran

Rostami¹,

Torbaghan²,

Dabiri³

et al. 2015

The American Society of Tropical Medicine and Hygiene

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Abstract. Cystic echinococcosis (CE), caused by the larval stage of Echinococcus granulosus, presents an important medical and veterinary problem globally, including that in Iran. Different genotypes of E. granulosus have been reported from human isolates worldwide. This study identifies the genotype of the parasite responsible for human hydatidosis in three provinces of Iran using formalin-fixed paraffin-embedded tissue samples. In this study, 200 formalin-fixed paraffin-embedded tissue samples from human CE cases were collected from Alborz, Tehran, and Kerman provinces. Polymerase chain reaction amplification and sequencing of the partial mitochondrial cytochrome c oxidase subunit 1 gene were performed for genetic characterization of the samples. Phylogenetic analysis of the isolates from this study and reference sequences of different genotypes was done using a maximum likelihood method. In total, 54.4%, 0.8%, 1%, and 40.8% of the samples were identified as the G1, G2, G3, and G6 genotypes, respectively. The findings of the current study confirm the G1 genotype (sheep strain) to be the most prevalent genotype involved in human CE cases in Iran and indicates the high prevalence of the G6 genotype with a high infectivity for humans. Furthermore, this study illustrates the first documented human CE case in Iran infected with the G2 genotype.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genetic Characterization of Echinococcus granulosus from a Large Number of Formalin-Fixed, Paraffin-Embedded Tissue Samples of Human Isolates in Iran

Rostami¹,

Torbaghan²,

Dabiri³

et al. 2015

The American Society of Tropical Medicine and Hygiene

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“…Analysis of partial mitochondrial ND1 sequence data revealed a C/T change at position 195 in human, sheep and cattle isolates (HQ717151) that has been reported in many isolates from various parts of the world (including HM853645, DQ856470 and AF408688, among others) and from Turkey (13,(45)(46)(47). The co-occurrence of the 195 C/T and 288 T/C changes (HQ717152) has been previously noted [FJ796212 (22)].…”

Section: Discussionmentioning

confidence: 76%

Molecular Characterization of Human and Animal Isolates of Echinococcus granulosus in the Thrace Region, Turkey

Eryıldız¹,

Şakru²

2012

Balkan Med J

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“…These questions have subsequently been resolved with the advent of reliable, robust and reproducible molecular tools which have not only supported the early taxonomy, but also demonstrated the distinctness of transmission cycles and the potential for interaction, particularly with respect to zoonotic transmission [95,103,106]. Importantly, these molecular epidemiological studies have given confidence to the morphological characters used for species discrimination which now offer a simple, cost effective means of parasite identification in endemic foci where the application of molecular tools may not be practical or cost-effective [107,108].…”

Section: Echinococcus Giardia and Cryptosporidiummentioning

confidence: 99%

The molecular epidemiology of parasite infections: Tools and applications

Lymbery

Thompson

2012

Molecular and Biochemical Parasitology

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Molecular epidemiology, broadly defined, is the application of molecular genetic techniques to the dynamics of disease in a population. In this review, we briefly describe molecular and analytical tools available for molecular epidemiological studies and then provide an overview of how they can be applied to better understand parasitic disease. A range of new molecular tools have been developed in recent years, allowing for the direct examination of parasites from clinical or environmental samples, and providing access to relatively cheap, rapid, high throughput molecular assays. At the same time, new analytical approaches, in particular those derived from coalescent theory, have been developed to provide more robust estimates of evolutionary processes and demographic parameters from multilocus, genotypic data. To date, the primary application of molecular epidemiology has been to provide specific and sensitive identification of parasites and to resolve taxonomic issues, particularly at the species level and below. Population genetic studies have also been used to determine the extent of genetic diversity among populations of parasites and the degree to which this diversity is associated with different host cycles or epidemiologically important phenotypes. Many of these studies have also shed new light on transmission cycles of parasites, particularly the extent to which zoonotic transmission occurs, and on the prevalence and importance of mixed infections with different parasite species or intraspecific variants (polyparasitism). A major challenge, and one which is now being addressed by an increasing number of studies, is to find and utilise genetic markers for complex traits of epidemiological significance, such as drug resistance, zoonotic potential and virulence.

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Sequence analysis of cox1 and nad1 genes in Echinococcus granulosus G3 genotype in camels (Camelus dromedarius) from central Iran

Cited by 65 publications

References 39 publications

Genetic Characterization of Echinococcus granulosus from a Large Number of Formalin-Fixed, Paraffin-Embedded Tissue Samples of Human Isolates in Iran

Genetic Characterization of Echinococcus granulosus from a Large Number of Formalin-Fixed, Paraffin-Embedded Tissue Samples of Human Isolates in Iran

Molecular Characterization of Human and Animal Isolates of Echinococcus granulosus in the Thrace Region, Turkey

The molecular epidemiology of parasite infections: Tools and applications

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