Separation of vitamin B complex and folic acid using TLC plates impregnated with some transition metal ions

Bhushan, Ravi; Parshad, Vineeta

doi:10.1002/bmc.1130080411

Cited by 19 publications

(6 citation statements)

References 8 publications

(1 reference statement)

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“…Literature reveals that vitamins B were determined by expensive and sophisticated techniques such as high-performance liquid chromatography (HPLC) [12,23,24], but there are few reports on separation of vitamins B with this resolution and speed of separation at low cost. Well-defined bands with typical peak shape, compact boundaries, and excellent resolution were obtained when the plate was developed with the aforementioned optimum mobile phase system (Fig.…”

Section: Resultsmentioning

confidence: 99%

Isolation and quantitative analysis of B1, B2, B6 AND B12 vitamins using high-performance thin-layer chromatography

2011

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A new, simple and accurate high-performance thin-layer chromatography (HPTLC) method has been developed for the separation and simultaneous analysis of B1, B2, B6 and B12 vitamins. Standard and sample solutions of vitamins were applied onto precoated aluminum-backed silica gel G 60 F254 HPTLC plate. The plates were developed with about thirty different solvent systems, and the ethanol -chloroform -acetonitrile -toluene -ammonia -water (7 : 4 : 4.5 : 0.5 : 1 : 1, v/v) mixture was chosen as the optimum mobile phase. UV detection was performed densitometrically at 254 nm. The retention factors of B1, B2, B6 and B12 vitamins were 0.36, 0.60, 0.85 and 0.46, respectively. The linearity range for indicated compounds was 10 -2500 ng per spot and the correlation coefficients were R 2 = 0.97 -0.98. The limits of quantification (LOQ) were 141.72, 42.41, 100.31 and 11.50 ng and the limits of detection (LOD) were 42.52, 12.72, 30.09 and 3.45 ng for B1, B2, B6 and B12 vitamins, respectively. Analyses of standard solutions showed good precision and repeatability.

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Section: Resultsmentioning

confidence: 99%

Isolation and quantitative analysis of B1, B2, B6 AND B12 vitamins using high-performance thin-layer chromatography

2011

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“…The methods present in [12][13] are only capable to analyze vitamin B1, vitamin B6 and vitamin B12 at the same time. However, the proposed method's superior points are coming from its simplicity and analyzing five vitamins which are vitamin B1, vitamin B2, vitamin B3, vitamin B6 and vitamin C simultaneously in oral powder for veterinary consumption that is not present in the existing methods [3][4][5]. Beside this, being a stability indicating method is another superior side of choosing the suggested method.…”

Section: Methods Developmentmentioning

confidence: 99%

“…Vitamins are organic compounds that are essential constituents of food required for normal growth, self maintenance and functioning of human and animal bodies. Different methods have been reported for determination of B-Complex vitamins ranging from derivative UV Spectrophotometry [1][2][3], reverse phase TLC [4], Electrophoresis [5] and reverse phase (RP)-HPLC. The standard methods are sometimes non-specific and time consuming, involve pre-treatment of the sample through complex chemical, physical or biological reactions to eliminate the interferences followed by individual methods for each different vitamin.…”

Section: Introductionmentioning

confidence: 99%

A novel RP-HPLC method for simultaneous determination of vitamins B1, B2, B3, B6 and C in oral powder for veterinary consumption

Hasımoglu¹,

Ghodke²

2018

jrp

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The water-soluble vitamins are gaining rapid importance not only for human health but also being widely recommended for animal health care because of theirs wide spectrum of activities ranging from deficiency, pathogenic condition to nutritional supplements. A plethora of articles are published for simultaneous evaluation of B-Complex Vitamins, but most of their methods are not selective and time consuming which involve pre-treatment of sample due to complexities involved in the placebo matrix. In this study, we propose a simple, specific, precise, accurate and linear method to separate vitamin B1 (thiamine), vitamin B2 (riboflavin sodium phosphate), vitamin B3 (niacin amide), vitamin B6 (pyridoxine) and vitamin C (ascorbic acid) by using Zorbax XDB C18 column (250 mm x 4.6 mm i.d., 5µm). The flow rate was kept as 1.3 mL/min and monitored at 270 nm with ambient temperature. Effective separations and quantification were obtained in 30 minutes with 10 µl injection volume. The current reverse phase gradient method resulted in significant resolution between interested vitamins and placebo matrix. Analytical method development, followed by analytical method validation was accomplished according to International Conference of Harmonization guidelines (ICH). Statistical evaluation of validation parameters proved that the results were highly specific, precise, accurate, robust and linear over a wide concentration range. This method has been validated and found to be applicable in routine analysis of vitamin B1, vitamin B2, vitamin B3, vitamin B6 and vitamin C in oral powder for veterinary consumption.

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“…15 By using any of the above developed solvent systems, vitamins which were not resolved on the untreated plates were resolved with most of the impregnating reagents. 15 By using any of the above developed solvent systems, vitamins which were not resolved on the untreated plates were resolved with most of the impregnating reagents.…”

Section: Bhushan and Parshadmentioning

confidence: 99%

Improved Separation of Vitamin B Complex and Folic Acid Using Some New Solvent Systems and Impregnated TLC

Bhushan

Parshad

1999

Journal of Liquid Chromatography & Related Technologies

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TLC separation of vitamins of the 'B' complex group and folic acid has been achieved on plates impregnated with different transition metal ions. The metal ions used were Mn ++ , Fe ++ , Co ++ , Ni ++ , Cu ++ , Zn ++ and Hg ++ . hR f values for all the vitamins, using six new solvent systems, worked out for the purpose, for each of the four different concentrations of each metal ion have been reported. The results have been discussed for each metal ion compared and the best conditions of separation have been identified.

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Separation of vitamin B complex and folic acid using TLC plates impregnated with some transition metal ions

Cited by 19 publications

References 8 publications

Isolation and quantitative analysis of B1, B2, B6 AND B12 vitamins using high-performance thin-layer chromatography

Isolation and quantitative analysis of B1, B2, B6 AND B12 vitamins using high-performance thin-layer chromatography

A novel RP-HPLC method for simultaneous determination of vitamins B1, B2, B3, B6 and C in oral powder for veterinary consumption

Improved Separation of Vitamin B Complex and Folic Acid Using Some New Solvent Systems and Impregnated TLC

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