Separation of the Antioxidant Compound Quercitrin from<i>Lindera obtusiloba</i>Blume and Its Antimelanogenic Effect on B16F10 Melanoma Cells

Hong, Chung Oui; Lee, Hyun Ah; Rhee, Chang-Ho; Choung, Se‐Young; Lee, Kwang Won

doi:10.1271/bbb.120562

Cited by 31 publications

(20 citation statements)

References 31 publications

(27 reference statements)

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“…Therefore, by ensnaring oxygen related radicals the MEMM could hamper their interaction with polyester fatty acids and would eliminate the enhancement of lipids peroxidative processes leading to MDA formation [36-39]. With regards to the contribution of the bioactive compounds towards the observed antioxidant activity of MEMM, the involvement of quercetin, quercitrin and rutin are worth mentioning based on previous reports [40-42]. …”

Section: Discussionmentioning

confidence: 99%

Methanol extract of Melastoma malabathricum leaves exerted antioxidant and liver protective activity in rats

Mamat

Kamarolzaman

Yahya

et al. 2013

BMC Complement Altern Med

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BackgroundMelastoma malabathricum L. (Melastomaceae) is a small shrub with various medicinal uses. The present study was carried out to determine the hepatoprotective activity of methanol extract of M. malabathricum leaves (MEMM) against the paracetamol-induced liver toxicity in rats model.MethodsThe respective chemicals and herbal solutions (10% DMSO, 200 mg/kg silymarin or MEMM (50, 250 and 500 mg/kg)) were administered orally to rats once everyday for 7 days followed by the hepatotoxicity assay. The blood samples and livers were collected and subjected to biochemical and microscopical analysis. Prior to the hepatoprotective study, MEMM was subjected to determination of the total phenolic content (TPC) and the antioxidant properties using several standard assays (e.g. 2, 2-diphenyl-1-picrylhydrazyl- and superoxide anion- radical scavenging assay, and oxygen radical absorbance capacity assay).ResultsMEMM exerted significant (p < 0.05) and high antioxidant activity in which high TPC was recorded; while in the hepatotoxicity study, the extract exhibited significant hepatoprotective effects against the paracetamol-induced hepatotoxic model. The results observed for serum liver enzymes (ALT, ALP and AST) as well as the microscopic observations and microscopic scoring supported the hepatoprotective potential of MEMM. The phytochemical and HPLC analysis of MEMM demonstrated the presence of flavonoids as its major constituents.ConclusionsThe MEMM-induced hepatoprotective activity could be allied partly to its antioxidant activity and the presence of flavonoids.

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Section: Discussionmentioning

confidence: 99%

Methanol extract of Melastoma malabathricum leaves exerted antioxidant and liver protective activity in rats

Mamat

Kamarolzaman

Yahya

et al. 2013

BMC Complement Altern Med

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“…[59] A recent study on separation of quercitrin from Lindera obtusiloba Blume indicated significant effects of quercitrin on antioxidant and anti-melanogenic activities in melanoma cells. [60] It is proposed that UREX might also carry some functions due to its noteworthy content of quercitrin as its marker compound.…”

Section: Yield Of Extractionmentioning

confidence: 99%

Total phenolic content and antioxidant activity of Ulam raja (Cosmos caudatus) and quantification of its selected marker compounds: Effect of extraction

Seyedreihani

Tan

Alkarkhi

et al. 2016

International Journal of Food Properties

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The effect of heating time (30, 45, and 60 min) and temperature (45, 65, and 85°C) on the extraction yield (total soluble solids) of Ulam raja (Cosmos caudatus) was studied using face-centered composite designresponse surface methodology. Total phenolic content and antioxidant activity (TEAC DPPH) of samples obtained by aqueous extraction under different temperature and time were determined using Folin-Ciocalteau and 2,2-diphenyl-1-picrylhydrazyl scavenging activity assays, respectively. The yield, total phenolic content, and TEAC DPPH of Ulam raja extracts ranged as 3.0-4.2°Brix, 36.09-37.76 mg gallic acid equivalent/g, and 121.17-146.84 µmol Trolox equivalent/g of plant material on dry basis, respectively. Yield and TEAC DPPH of samples were significantly influenced (p < 0.05) by the extraction conditions. Meanwhile, no significant effect (p > 0.05) on total phenolic content was observed. Extraction of Ulam raja at 85°C for 30 min resulted in higher yield and TEAC DPPH of the extract. The chromatographic and spectral data confirmed the presence of several flavonoids in the lyophilized Ulam raja extract, i.e., quercitrin, catechin, and rutin, and their quantities were also reported as 36.9, 25.0, and 8.2 mg/g of Ulam raja extract, respectively.

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“…Moreover, studies have demonstrated that those compounds which failed initially as antimicrobials, drastically enhanced the effectiveness ofother chemotherapeutic agent [16].Therefore, we have conducted this study using purified plant based compounds Quercitrin and Deoxynojirimycin (DNJ) in order to meet the need of efficiency with low toxicity levels [12], [17].Earlier studies have reported the significance of Quercitrin and DNJ in medicine. Quercitrin (quercetin 3- O-alpha- L-rhamnopyranoside), a flavonoid glycoside has been reported by many researchers for its wide array of pharmacological activities such as, anti-inflammatory [18], antileishmanial [19], antimelanogenic [20],prevention oflipid peroxidation [21] and protectiveagainst UVB-induced oxidative damage of skin [22].However, its activity against cariogenic properties of S. mutans has not been explored.Moreover, DNJis known to prevent diet-induced obesity [23], hepatitis C virus [24], modulate glucose metabolism and has anti-diabetic effects [25].DNJ, nevertheless, is known for its anti-biofilm effect but its activity and mechanism of action as pure compound is not clear [12].…”

Section: Introductionmentioning

confidence: 99%

Inhibition of Major Virulence Pathways of Streptococcus mutans by Quercitrin and Deoxynojirimycin: A Synergistic Approach of Infection Control

et al. 2014

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ObjectivesTo evaluate the synergistic effect of Quercitrin and Deoxynojirimycin (DNJ) together with their individual inhibitory effect against virulence pathways of Streptococcus mutans.MethodologyMICs of both the compounds were determined by the microdilution method, followed by their in vitrosynergy using checkerboard and time kill assay. The nature of interaction was classified as synergistic on the basis of fractional inhibitory concentration index (FICI) value of ≤0.5. Furthermore, the activity of Quercitrin and DNJ was evaluated individually and in combination against various cariogenic properties of S. mutans UA159 such as acidogenesis, aciduracity, glucan production, hydrophobicity, biofilm and adherence. Moreover, expression of virulent genes in S. mutans was analysed by quantitative RT- PCR (qRT-PCR) and inhibition of F1F0-ATPase, lactate dehydrogenase and enolase was also evaluated. Finally, scanning electron microscopy (SEM) was used to investigate structural obliteration of biofilm.ResultsThe in vitro synergism between Quercitrin and DNJ was observed, with a FICI of 0.313. Their MIC values were found to be 64 μg/ml and 16 μg/ml respectively. The synergistic combination consistently showed best activity against all the virulence factors as compared to Quercitrin and DNJ individually. A reduction in glucan synthesis and biofilm formation was observed at different phases of growth. The qRT-PCR revealed significant downregulation of various virulent genes. Electron micrographs depicted the obliteration of biofilm as compared to control and the activity of cariogenic enzymes was also inhibited.ConclusionsThe whole study reflects a prospective role of Quercitrin and DNJ in combination as a potent anticariogenic agent against S. mutans.

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Separation of the Antioxidant Compound Quercitrin fromLindera obtusilobaBlume and Its Antimelanogenic Effect on B16F10 Melanoma Cells

Cited by 31 publications

References 31 publications

Methanol extract of Melastoma malabathricum leaves exerted antioxidant and liver protective activity in rats

Methanol extract of Melastoma malabathricum leaves exerted antioxidant and liver protective activity in rats

Total phenolic content and antioxidant activity of Ulam raja (Cosmos caudatus) and quantification of its selected marker compounds: Effect of extraction

Inhibition of Major Virulence Pathways of Streptococcus mutans by Quercitrin and Deoxynojirimycin: A Synergistic Approach of Infection Control

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