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Separation of proteins by reversed-phase high-performance liquid chromatography
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Cited by 115 publications
(39 citation statements)
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“……”
Section: Ph-dependent Retention Time
mentioning
confidence: 55%
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“……”
Section: Ph-dependent Retention Time
mentioning
confidence: 55%
Abstract
Smart CitationsHow this paper cites the one you are viewing
“…Proteins are partly composed of amino acids that are hydrophobic; therefore, the hydrophobic contribution could be a reason for the considerable amount of BSA adsorbed on zirconia despite the repulsive zeta potential. Between the two proteins, BSA is overall less hydrophilic than LSZ as it can be concluded from surface tension measurements of protein solutions (Table 2) and from literature [47].…”
Section: Discussion
mentioning
confidence: 83%
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“…The adsorption of BSA shifts the IEP of the suspension from pH 8.7 down to pH 7. In contrast to this, the adsorption of LSZ shifts the IEP closer to pH 9 as expected from the LSZ IEP which is at pH 11 [11].…”
Section: Protein Adsorption
mentioning
confidence: 75%
Smart CitationsHow this paper cites the one you are viewing
“……”
Section: Ph-dependent Retention Time
mentioning
confidence: 55%
Abstract
Smart CitationsHow this paper cites the one you are viewing
“…Proteins are partly composed of amino acids that are hydrophobic; therefore, the hydrophobic contribution could be a reason for the considerable amount of BSA adsorbed on zirconia despite the repulsive zeta potential. Between the two proteins, BSA is overall less hydrophilic than LSZ as it can be concluded from surface tension measurements of protein solutions (Table 2) and from literature [47].…”
Section: Discussion
mentioning
confidence: 83%
Smart CitationsHow this paper cites the one you are viewing
“…The adsorption of BSA shifts the IEP of the suspension from pH 8.7 down to pH 7. In contrast to this, the adsorption of LSZ shifts the IEP closer to pH 9 as expected from the LSZ IEP which is at pH 11 [11].…”
Section: Protein Adsorption
mentioning
confidence: 75%
Smart CitationsHow this paper cites the one you are viewing
“……”
Section: Ph-dependent Retention Time
mentioning
confidence: 55%
Abstract
Smart CitationsHow this paper cites the one you are viewing
“…Proteins are partly composed of amino acids that are hydrophobic; therefore, the hydrophobic contribution could be a reason for the considerable amount of BSA adsorbed on zirconia despite the repulsive zeta potential. Between the two proteins, BSA is overall less hydrophilic than LSZ as it can be concluded from surface tension measurements of protein solutions (Table 2) and from literature [47].…”
Section: Discussion
mentioning
confidence: 83%
Smart CitationsHow this paper cites the one you are viewing
“…The adsorption of BSA shifts the IEP of the suspension from pH 8.7 down to pH 7. In contrast to this, the adsorption of LSZ shifts the IEP closer to pH 9 as expected from the LSZ IEP which is at pH 11 [11].…”
Section: Protein Adsorption
mentioning
confidence: 75%