Separation of collagen hydrolysate amino acids by ion-pair reversed-phase high-performance liquid chromatography

HPLC assay of underivatized free amino acids with column switching and evaporative light‐scattering detection

“…As shown in Table 2 same order of magnitude of the detection limits reported previously for Trp and Val [18, 191. They represent detection limits that are ten times lower than observed with OPA derivatives, more than twice as highe as the ones for ninhydrin detection [22], and are comparable to detection limits achieved by some gas chromatographic determinations [23].…”

HPLC assay of underivatized free amino acids with column switching and evaporative light‐scattering detection

“…As shown in Table 2 same order of magnitude of the detection limits reported previously for Trp and Val [18, 191. They represent detection limits that are ten times lower than observed with OPA derivatives, more than twice as highe as the ones for ninhydrin detection [22], and are comparable to detection limits achieved by some gas chromatographic determinations [23].…”

HPLC assay of underivatized free amino acids with column switching and evaporative light‐scattering detection

“…As shown in Table 2 same order of magnitude of the detection limits reported previously for Trp and Val [18, 191. They represent detection limits that are ten times lower than observed with OPA derivatives, more than twice as highe as the ones for ninhydrin detection [22], and are comparable to detection limits achieved by some gas chromatographic determinations [23].…”

HPLC assay of underivatized free amino acids with column switching and evaporative light‐scattering detection

“…As shown in Table 2 same order of magnitude of the detection limits reported previously for Trp and Val [18, 191. They represent detection limits that are ten times lower than observed with OPA derivatives, more than twice as highe as the ones for ninhydrin detection [22], and are comparable to detection limits achieved by some gas chromatographic determinations [23].…”