Separation of Alkaloids in<i>Chelidonium majus</i>by Reversed Phase HPLC

Táborská, Eva; Bochořáková, Hana; Paulová, Hana; Dostál, Jiřı́

doi:10.1055/s-2006-959508

Cited by 28 publications

(13 citation statements)

References 1 publication

(1 reference statement)

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“…Twenty-seven compounds from the aerial parts and roots of Ch. majus have been separated and identified by RP-HPLC with a complex gradient program [9]. RP ion-pair chromatography with different ion-pair reagents has been used for separation of allocryptopine, protopine, and chelidonine [10].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of seven main alkaloids of Chelidonium majus L. by ultra‐performance LC with photodiode‐array detection

Qian

Duan

et al. 2010

J of Separation Science

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A simple and rapid method for the simultaneous determination of seven isoquinoline alkaloids, protopine, chelidonine, coptisine, stylopine, sanguinarine, berberine, and chelerythrine, in Chelidonium majus L. (Ch. majus) samples by ultra-performance LC method with photodiode array detection is described. The baseline separation of these compounds was performed with (A) acetonitrile-(B) ammonium acetate (10 mM, adjusted to pH 3.0 with acetic acid) as the mobile phase using a C18 RP column (2.1x100 mm, 1.7 microm). Optimized conditions resulted in excellent peak shapes. The seven alkaloids were completely separated within 20 min. Good linear behaviors (r > or = 0.9992) over the investigated concentration ranges were observed for all the analytes. Validation proved the repeatability of the method was good and recovery was satisfactory. The validated method was successfully applied for 20 batches of Ch. majus. These results demonstrated that the ultra-performance LC photodiode array method proposed was very useful in the analysis and quality control of Ch. majus.

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Section: Introductionmentioning

confidence: 99%

Simultaneous determination of seven main alkaloids of Chelidonium majus L. by ultra‐performance LC with photodiode‐array detection

Qian

Duan

et al. 2010

J of Separation Science

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“…The separation of about 30 alkaloids of Chelidonium majus L. cannot be achieved in a single isocratic chromatographic system. Lastly, Taborska et al [14] using reversedphase ion-pair HPLC and a 60 min gradient program resolved 27 Chelidonium majus L. alkaloids. This method can be recommended for the routine evaluation of plant material on an analytical scale, but not for the micropreparative isolation of the individual alkaloids.…”

Section: Resultsmentioning

confidence: 99%

“…No alkaloids were present in the first portion of the eluate, but in subsequent ones protopine, chelidonine, allocryptopine, homochelidonine and a few minor alkaloids were found ( Figure 4). It should be stressed that the concentration of the tertiary alkaloids in the eluate is 2-3 times higher than in the extract introduced onto the column [ 14].…”

Section: Figurementioning

confidence: 94%

Isolation of some quaternary alkaloids from the extract of roots ofChelidonium Majus L. by column and thin-layer chromatography

Gołkiewicz

Gadzikowska

1999

Chromatographia

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Key WordsColumn liquid chromatography. Micropreparative column liquid chromatography Micropreparative thin-layer chromatography Alkaloids of Chelidonium majus L. SummaryA chromatographic system comprising untreated silica gel and an aqueous buffer -methanol eluent was investigated for the micropreparative separation of the alkaloids of Chelidonium majus L. The relationship between the logarithm of the retention factors k of the alkaloids and volume fraction of methanol at pH* 6 was linear for volume fractions in the range of 0.1 to 0.4. From this relationship it is possible to estimate the value of the retention factor for a given alkaloid in pure buffer and then the maximum volume of alkaloid extract that can be sampled on micropreparative column filled with silica gel.During the last decade, unmodified silica gel with aqueous buffer -organic solvent mixtures has been successfully applied to the separation of a large number of organic base mixtures [3][4][5][6][7][8][9][10][11]. This chromatographic system, called ,,pseudo reversed phase" (9) was also applied to the separation of alkaloids of different classes [3,7,10,11]. It was shown that this chromatographic system can also be used to preconcentrate quaternary alkaloids or to separate quaternary and tertiary alkaloids contained in crude extracts of Chelidonium majus L. into two groups [ 11 ]. Preconcentration of alkaloids from aqueous extracts on columns filled with Amberlite XAD or chemically bonded organic stationary phases, e.g., C18, is very expensive because injection of plant extracts containing strongly retained solutes degrades column performance. This paper presents the results of systematic studies on the choice of conditions for the micropreparative separation and purification of the main quaternary C. majus alkaloids from crude extracts. The aim of this investigations was to eliminate as many organic solvents as possible, especially halogen containing ones, from the isolation process of the alkaloids and to use a simple and inexpensive chromatographic system composed of bare silica gel and an aqueous buffer -organic solvent mobile phase.

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“…This is a group of biologically active heterocyclic nitrogen-containing substances. Extract of celandine (C. majus L.) contains about 35 alkaloids [1,4] with protopinic, protoberberin and benzophenanthridine structure. Among alkaloids contained in the extracts of C. majus are chelidonin, sanguinarine, berberin, narcissine and coptisin.…”

Section: Introductionmentioning

confidence: 99%

Influence of Chelidonium majus Water Extract onto Cancer Cells and Platelets

Vn¹,

Ry²,

Vo³

et al. 2018

Biochem Anal Biochem

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The plant C. majus (Celandine) is a known source of biologically active compounds that are used in traditional medicine for preventing or curing cancer diseases. The growing interest in this plant is due to discovery of a number of plant-derived cytostatics and anti-blastic drugs. The goal of our study was to discover the influence of a water extract from these plants on cancer cells and to characterize the obtained extract using different analytical methods. Water extract of leaves of C. majus were obtained and lyophilized on TelStar LyoQuest (Spain). Composition of the extract was characterized using HPLC Agilent 1100, SDS-PAGE electrophoresis and MLADI-TOF Mass-spectrometry. ADP-induced platelet aggregation in the presence of obtained mixture was studied by aggregometry using Solar2110 (Belorussia). Effects of the extracts on proliferation of cancer cells was estimated using MTT-test. Investigation of C. majus extract demonstrated presence of chelerythrine and precursors of berberine alkaloids in it. Addition of C. majus extract to platelet rich plasma induced moderate platelet activation (up to 10%). This aggregation was reversible. Further ADP-induced activation of platelets pre-incubated with the extract was diminished on 30%. The extract of C. majus leaves also was shown to possess anti-proliferative agent that was demonstrated on MCF-7 cancer cells. The surviving of MCF-7 coulter cells was decreased to 40% in the presence of extract. In conclusion, direct action of C. majus extract components on platelet aggregation and cancer cell proliferation was observed. Further fractionation for the determination of functionally active components of C. majus extract is promising for biotechnology and medicine.

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Separation of Alkaloids inChelidonium majusby Reversed Phase HPLC

Cited by 28 publications

References 1 publication

Simultaneous determination of seven main alkaloids of Chelidonium majus L. by ultra‐performance LC with photodiode‐array detection

Simultaneous determination of seven main alkaloids of Chelidonium majus L. by ultra‐performance LC with photodiode‐array detection

Isolation of some quaternary alkaloids from the extract of roots ofChelidonium Majus L. by column and thin-layer chromatography

Influence of Chelidonium majus Water Extract onto Cancer Cells and Platelets

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