Separation of Alkaloids by Gas Chromatography

Genetical control of alkaloids in Lupinus albus

“…method was developed from techniques described by CRANMER &MABRY (1966) andLLOYD et al, (1960). Seven-foot glass columns with 4 mm internal diameters using three phases -3~ S.E.…”

Determination of Steroids by Gas-Liquid Cinematography

“…Tritium-labeled estriol was added as internal standard before hydrolysis. Acid hydrolysis was performed according to Brown and Blair [26], with 15 volume per cent concentrated hydrochloric acid refluxed for 1 h. The hydrolyzed urine was extracted 3 times with 50 ml distilled diethyl ether. The combined ether extracts were washed once with 50 ml saturated sodium bicarbonate.…”

“…Since then the application of gas chrom atography has expanded rapidly. The separation and quantification of many classes of compounds was accomplished, including vitam ins [53], alkaloids [26], human skin wax esters [20], derivatives of sugars [43]. amino acids and nucleosides [31,34], drugs and drug metabolites [1,19,45].…”

Gas–Liquid Chromatography of Heterocyclic Compounds

“…The treatment of the solid support with alkali as a method of suppressing acid sites proved very fruitful in separating alkaloids. 16 " 21 The investigation of the Chromatographie behavior of HC (pyridine, furan, thiophene derivatives) on columns packed with the alkali-treated solid support revealed that alkali markedly reduced the solid support specific area, decreased discernibly retention times and effectively eliminated tailing effects in the case of pyridine bases; moreover, alkali participated in the separation process bringing about specific interactions. [22][23][24][25][26][27] In studying the separation of amines and nitrogen-containing HC (pyridine, piperidine, pyrrolidine, pyrrole, picolines), Golovnya et al likewise faced a problem of the specific influence of alkali on the liquid phase properties (polarity increase).…”