Journal of Chromatography A 2012 DOI: 10.1016/j.chroma.2012.01.063 View full text
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Deidre L. Blackwell, Scott R. Bean

Abstract: Kafirins, the storage proteins and major protein of the cereal grain sorghum, play an important nutritional role for millions of people in parts of Africa and Asia. Kafirins are non-water soluble, being soluble only in the presence of detergents or aqueous alcohol mixtures and are among the most hydrophobic of the cereal proteins. Limited M(w) heterogeneity of kafirins reduces their resolution when separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Charge based separation techni…

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