Separation and purification of baicalin and wogonoside from the Chinese medicinal plant Scutellaria baicalensis Georgi by high-speed counter-current chromatography

Wu, Sujuan; Sun, Ailing; Liu, Renmin

doi:10.1016/j.chroma.2005.01.054

Cited by 75 publications

(49 citation statements)

References 2 publications

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“…The rhizome of Scutellaria baicalensis (SB), which contains baicalin and wogonoside as the main constituents, has long been used in China, Japan and Korea as a traditional medicine and functional food for inflammation, fever, hepatitis, allergic disease, hypertension, etc (Zhu, 1998;Wu et al, 2005). These constituents exhibit anti-inflammatory, antiallergic, anti-oxidant, and hepatoprotective, and antitumor effects (Chou et al, 2003;Jang et al, 2003;Lim, 2003;Kim et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Metabolism of Wogonoside by Human Fecal Microflora and Its Anti-pruritic Effect

Trinh¹,

Jang²,

Han³

et al. 2009

Biomolecules and Therapeutics

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-To understand the relationship between the metabolism of wogonoside from the rhizome of Scutellaria baicalensis, and its anti-pruritic effect, we anaerobically incubated it with human fecal microflora, identified its metabolite identified, and investigated its anti-pruritic effect in compound 48/80 or histamineinduced pruritic mice. Wogonoside was metabolized to wogonin, with metabolic activity of 6.9 ± 5.1 nmol/h/ mg wet weight of fecal microflora. Orally administered wogonoside had more potent anti-scratching behavioral effect in compound 48/80 or histamine-treated mice than intraperitoneally treated one, apart from orally administered its metabolite, wogonin, which was more potent than the orally administered one. Wogonoside showed more potent anti-pruritic effects when administered at 5 h prior to the pruritic agent treatment than when administered at 1 h before. However, wogonin orally administered 1 h before the treatment with pruritic agents showed a more potent anti-pruritic effect than when treated at 5 h before. Orally administered wogonoside may be metabolized to wogonin in the intestine and its anti-scratching behavioral effect may be dependent on its metabolism by intestinal microflora.

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Section: Introductionmentioning

confidence: 99%

Metabolism of Wogonoside by Human Fecal Microflora and Its Anti-pruritic Effect

Trinh¹,

Jang²,

Han³

et al. 2009

Biomolecules and Therapeutics

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“…To evaluate the anti-pruritic effect of agents, histamine or substance P-induced scratching behavioral mouse models were used [1][2][3][4][5] . The rhizome of Scutellaria baicalensis (SB), which contains baicalin as a main constituent, has long been used in China, Japan, and Korea as a traditional medicine and functional food for inflammation, fever, hepatitis, allergic disease, hypertension, etc [6,7] . Baicalin exhibits anti-inflammatory, anti-allergic, anti-oxidant, hepatoprotective, and anti-tumor effects [8][9][10] .…”

Section: Introductionmentioning

confidence: 99%

Anti-pruritic effect of baicalin and its metabolites, baicalein and oroxylin A, in mice

et al. 2010

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Aim: To explore whether intestinal microflora plays a role in anti-pruritic activity of baicalin, a main constituent of the rhizome of Scutellaria baicalensis (SB). Methods: Baicalin was anaerobically incubated with human fecal microflora, and its metabolites, baicalein and oroxylin A, were isolated. The inhibitory effect of baicalin and its metabolites was accessed in histamine-or compound 48/80-induced scratching behavior in mice. Results: Baicalin was metabolized to baicalein and oroxylin A, with metabolic activities of 40.2±26.2 and 1.2±1.1 nmol·h -1 ·mg -1 wet weight of human fecal microflora, respectively. Baicalin (20, 50 mg/kg) showed more potent inhibitory effect on histamine-induced scratching behavior when orally administered than intraperitoneally. In contrast, baicalein and oroxylin A had more potent inhibitory effect when the intraperitoneally administered. The anti-scratching behavior activity of oral baicalin and its metabolites was in proportion to their inhibition on histamine-induced increase of vascular permeability with oroxylin A more potent than baicalein and baicalin. In Magnus test using guinea pig ileum, oroxylin A is more potent than baicalein and baicalin in inhibition of histamine-induced contraction. The anti-scratching behavioral effect of oral baicalin was significantly reduced when oral antibiotics were simultaneously administered, whereas the effect of baicalein and oroxylin A were not affected. Conclusion: Oral baicalin may be metabolized by intestinal microflora into baicalein and oroxylin A, which ameliorate pruritic reactions through anti-histamine action.

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“…Flavonoids are the main active components of S. baicalensis. Among them, baicalin and wogonoside are the main flavonoids [24]. Pharmacological studies have indicated that baicalin and wogonoside share many beneficial activities with Radix scutellariae, concerning the anti-inflammatory, antiallergic, antioxidant, and hepatoprotective properties [25 -28].…”

Section: ) Gave a [M à H]mentioning

confidence: 99%

Characterization of the Multiple Absorbed Constituents in Rats after Oral Administration of Chai‐Huang Decoction by Liquid Chromatography Coupled with Electrospray‐Ionization Mass Spectrometry

Guan

Tang

Wang

et al. 2010

Chemistry & Biodiversity

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A rapid, sensitive, and specific method by high-performance liquid chromatography (HPLC) coupled to diode-array detection (DAD) and tandem mass spectrometry (MS) techniques was developed for the identification of absorbed constituents and their metabolites in rats after the oral administration of a Chai-Huang decoction (CHD), which consists of Bupleurum chinense and Scutellaria baicalensis in the proportion 1 : 1 (w/w). By comparing their retention times and MS data with those of authentic compounds and published data, a total of 14 compounds were identified in the CHD samples. In addition, eleven and seven compounds were characterized in the urine and serum samples of the rats, respectively. The results indicated that the main absorbed constituents were chrysin-6-C-arabinosyl-8-C-glucoside, chrysin-6-C-glucosyl-8-C-arabinoside, baicalin, wogonin-5-O-glucoside, oroxylin A-7-O-glucuronide, wogonoside, saikosaponin A, saikosaponin C, saikosaponin D, baicalein, and wogonin. These compounds might be responsible for the curative effects of the CHD. The findings demonstrated that the proposed method could be used to rapidly and simultaneously analyze and screen the multiple absorbed bioactive constituents in a formula of traditional Chinese medicines (TCM). This is very important not only for the pharmaceutical discovery process and the quality control of crude drugs but also to explain the mechanisms of action of TCM.

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Separation and purification of baicalin and wogonoside from the Chinese medicinal plant Scutellaria baicalensis Georgi by high-speed counter-current chromatography

Cited by 75 publications

References 2 publications

Metabolism of Wogonoside by Human Fecal Microflora and Its Anti-pruritic Effect

Metabolism of Wogonoside by Human Fecal Microflora and Its Anti-pruritic Effect

Anti-pruritic effect of baicalin and its metabolites, baicalein and oroxylin A, in mice

Characterization of the Multiple Absorbed Constituents in Rats after Oral Administration of Chai‐Huang Decoction by Liquid Chromatography Coupled with Electrospray‐Ionization Mass Spectrometry

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