Separation and Identification of Some Cephalosporins on Impregnated TLC Plates

Bhushan, Ravi; Parshad, Vineeta

doi:10.1002/(sici)1099-0801(199609)10:5<258::aid-bmc597>3.0.co;2-g

Cited by 15 publications

(6 citation statements)

References 3 publications

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“…A variation of this method is the impregnation of layers with metal ions that act as central atoms. For example, thin-layer plates impregnated with cadmium, copper, zinc, or manganese salts have been used to separate amino acids (211), aromatic amines (212), humic acids (213), peptides (214), phenolics (215), and sulfonamides (216). Also, thin-layer plates can be impregnated with various organic compounds such as salicylic acid, syringic acid, o-phthalic acid, and phenolic acids to separate various metal ions such as Cu 2r , Fe 3^, Hg + , Pb + , and Ni + (217)(218)(219).…”

Section: Rabelmentioning

confidence: 99%

Sorbents and Precoated Layers in Thin-Layer Chromatography

Rabel¹

2003

Handbook of Thin-Layer Chromatography

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Section: Rabelmentioning

confidence: 99%

Sorbents and Precoated Layers in Thin-Layer Chromatography

Rabel¹

2003

Handbook of Thin-Layer Chromatography

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“…In the analysis of cephalosporins, TLC is employed for identification purposes and purity control, as well as for stability and metabolism studies. It is also the method of choice for monitoring the cross-contamination of cephalosporins with traces of penicillin [29]. A TLC technique with densitometric detection was used to isolate and quantify CFZ from serum and urine.…”

Section: Introductionmentioning

confidence: 99%

Development of Thin-Layer Chromatography–Densitometric Procedure for Qualitative and Quantitative Analyses and Stability Studies of Cefazolin

Żandarek,

Starek,

Dąbrowska

2024

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Cefazolin is a first-generation cephalosporin used to treat severe infections of the respiratory tract, urinary tract, skin, and soft tissues. This study presents the optimal conditions for the determination of cefazolin by thin-layer chromatography with densitometric detection. A chloroform–methanol–glacial acetic acid mixture (6:4:0.5, v/v/v) was selected as the mobile phase, while TLC silica gel 60F254 plates were used as the stationary phase. Next, the developed procedure was validated in accordance with ICH guidelines. The obtained results showed that the method is selective, precise, and accurate in a linearity range of 0.04–1.00 µg/spot (r > 0.99). Subsequently, qualitative and quantitative analyses of formulations containing cefazolin were performed. It was found that the amount of antibiotic is highly consistent with the content declared by manufacturers. The suitability of the developed method for stability testing under varying environmental conditions was also verified. It was found that under the tested conditions, the degradation process follows first-order kinetics. The lowest stability was registered in an alkaline environment and in the presence of an oxidizing agent, and the highest stability was recorded in water, and these results were confirmed by the calculated kinetic parameters. The developed method can be used in qualitative and quantitative analyses and stability studies of the analyzed antibiotic.

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“…Of greatest interest is the analysis of cephalosporins both in pure form and in pharmaceuticals and biological fluids [ 1 , 25 ]. Today, chromatographic techniques are widely used for the analysis of cephalosporins, including high-performance liquid chromatography (HPLC) [ 26 , 27 , 28 , 29 , 30 , 31 ], ultra-high performance liquid chromatography (UPLC) [ 14 ], high-performance thin layer chromatography [ 32 ], and capillary electrophoresis (CE) [ 33 , 34 , 35 , 36 ].…”

Section: Introductionmentioning

confidence: 99%

Analytical Determination of Cephalosporin Antibiotics Using Coordination Polymer Based on Cobalt Terephthalate as a Sorbent

et al. 2023

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In this work, a coordination polymer based on cobalt terephthalate was obtained and characterized by elemental analysis, infrared spectroscopy, X-ray diffraction analysis, and scanning electron microscopy. The coordination polymer was tested as a sorbent for the solid-phase extraction of cephalosporin antibiotics, including ceftriaxone, cefotaxime, and cefazolin, from aqueous solutions. The coordination polymer had a high adsorption capacity (520.0 mg/g). Antibiotics adsorption followed pseudo-second order kinetic model and the Freundlich isotherm model. The calculated thermodynamic parameters indicate a spontaneous process. The resulting coordination polymer has good stability and reusability. The possibility of separating the studied cephalosporins on a chromatographic column filled with a coordination polymer was shown. This work opens great prospects for the development and application of a coordination polymer based on cobalt terephthalate for the removal of cephalosporins from ambient water.

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Separation and Identification of Some Cephalosporins on Impregnated TLC Plates

Cited by 15 publications

References 3 publications

Sorbents and Precoated Layers in Thin-Layer Chromatography

Sorbents and Precoated Layers in Thin-Layer Chromatography

Development of Thin-Layer Chromatography–Densitometric Procedure for Qualitative and Quantitative Analyses and Stability Studies of Cefazolin

Analytical Determination of Cephalosporin Antibiotics Using Coordination Polymer Based on Cobalt Terephthalate as a Sorbent

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