Separating Stem Cells by Flow Cytometry: Reducing Variability for Solid Tissues

Alexander, Caroline M.; Puchalski, Joel R.; Klos, Kristine S.; Badders, Nisha M.; Ailles, Laurie; Kim, Carla F.; Dirks, Peter B.; Smalley, Matthew J.

doi:10.1016/j.stem.2009.11.008

Cited by 55 publications

(45 citation statements)

References 15 publications

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“…The isolation of adult stem cells in general-and MaSCs in particular-using multichannel flow cytometry is subject to variability from limited antibody specificity and technical variance (Alexander et al 2009). In addition, the distribution of mammary tissue cells, based on cell surface marker expression, changes with the developmental phase (see Fig.…”

Section: Discussionmentioning

confidence: 99%

s-SHIP promoter expression marks activated stem cells in developing mouse mammary tissue

Bai¹,

2010

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Mammary stem cells (MaSCs) play critical roles in normal development and perhaps tumorigenesis of the mammary gland. Using combined cell markers, adult MaSCs have been enriched in a basal cell population, but the exact identity of MaSCs remains unknown. We used the s-SHIP promoter to tag presumptive stem cells with GFP in the embryos of a transgenic mouse model. Here we show, in postnatal mammary gland development, that GFP [Keywords: Stem cell; mammary gland development; cap cells; alveolar bud cells; s-SHIP; breast tumors] Supplemental material is available at http://www.genesdev.org.

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Section: Discussionmentioning

confidence: 99%

s-SHIP promoter expression marks activated stem cells in developing mouse mammary tissue

Bai¹,

2010

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“…tumors were analyzed on different days. In compliance with Alexander et al 49 single color controls (Lin-Cy5, CD24-FItC, CD49f-pe or pI) were included in each experiment and the compensation was done by the software. (B) Unsupervised hierarchical cluster analysis of gene expression data from tumors outgrown after sorting.…”

Section: Discussionmentioning

confidence: 99%

Tumor-initiating cells are not enriched in cisplatin-surviving BRCA1;p53-deficient mammary tumor cells in vivo

et al. 2010

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“…Smalley gave a talk on the pros and cons of FACS and in situ lineage tracing as approaches to the analysis of mammary epithelial subpopulations (Table 1) [4]. The importance of caution in data interpretation and being able to make ones experiments cross-comparable with results from other groups was emphasised.…”

Section: Day Twomentioning

confidence: 99%

See One, Do One, Teach One: A Practical Course on Methods in Mammary Gland Biology

Jechlinger

Kendrick

Smalley

et al. 2017

J Mammary Gland Biol Neoplasia

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The first EMBO Practical Course on "Techniques for Mammary Gland Research" took place at EMBL, Heidelberg, from the 20th -24th March 2017. The general aim of this practical and theory course was to ensure that all participants had the opportunity to learn about and practice some of the classical assays for studying mammary gland biology and tumorigenesis and also to enjoy some of the latest state-ofthe-art technology within the imaging facilities at EMBL. Specific goals for the course were to describe, demonstrate and practice key techniques for studying human and mouse mammary gland biology, with special emphasis on discussing both systems in the context of analysis of stem/progenitor cell populations. The course hosted 20 participants from 15 different countries, providing an additional excellent opportunity for scientific exchange and networking. Day OneOur first day's events were all located at the excellent ISG Hotel. Having made ourselves comfortable, we were briefed on how the course would work by Jacqueline Dreyer-Lamm, from EMBL. Dr. Maria Vivanco from CIC bioGUNE, Bilbao, introduced the course and its origins as an extension of the Workshop that the European Network of Breast Development and Cancer Labs (ENBDC, http://www.enbdc .org) organises every year in Weggis, Switzerland. It was the intention of this practical course to compliment the Weggis meeting, which is dedicated to oral presentations with a focus on methodology.We were then fortunate enough to have Professor Nancy Hynes from the Friedrich Miescher Institute for Biomedical Research, Basel, as a guest speaker. Prof. Hynes gave an inspiring overview of her career in breast cancer research, with a particular focus on the importance of novel methodological approaches in making breakthrough discoveries.

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Separating Stem Cells by Flow Cytometry: Reducing Variability for Solid Tissues

Cited by 55 publications

References 15 publications

s-SHIP promoter expression marks activated stem cells in developing mouse mammary tissue

s-SHIP promoter expression marks activated stem cells in developing mouse mammary tissue

Tumor-initiating cells are not enriched in cisplatin-surviving BRCA1;p53-deficient mammary tumor cells in vivo

See One, Do One, Teach One: A Practical Course on Methods in Mammary Gland Biology

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