Sensitivity to methyl benzimidazole carbamate fungicides of Botryosphaeriaceae species from mango orchards in the Northeast of Brazil

Santos, Kledson Mendes dos; Tsuji, Susan Satie; Câmara, Marcos Paz Saraiva; Michereff, Sami Jorge; Lopes, Ueder Pedro

doi:10.1007/s10658-018-1556-y

Cited by 9 publications

(2 citation statements)

References 36 publications

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“…All isolates (S and LS) were able to maintain their level of sensitivity to imazalil and thiabendazole after 10 successive transfers on fungicide‐free medium. This fact has also been demonstrated in other studies of DMIs and MBCs, with no reduction in sensitivity after subcultures in the absence of fungicides (Chen et al ., 2013; Santos et al ., 2019). This stability of sensitivity level is a concern, because LS isolates can remain in the population after successive cycles, even in the absence of fungicides.…”

Section: Discussionmentioning

confidence: 99%

Analysis of Colletotrichum musae populations from Brazil reveals the presence of isolates with high competitive ability and reduced sensitivity to postharvest fungicides

et al. 2020

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In this study, the sensitivity of 218 isolates of Colletotrichum musae to imazalil and thiabendazole was evaluated, as well the fitness and competitive ability of less sensitive isolates. There was a positive correlation between the sensitivity to the two fungicides, but the isolates were more sensitive to imazalil. The estimated effective concentration of the fungicide able to inhibit mycelial growth by 50% (EC50) was used to select four isolates with the lowest and the highest values for both fungicides, which were considered as sensitive (S) and less sensitive (LS), respectively. The level of sensitivity was maintained after 10 successive transfers on fungicide‐free medium. Both fungicides were effective in controlling the disease caused by S isolates of C. musae in detached banana fruit when recommended doses were used. However, only imazalil was able to control the disease caused by LS isolates. For both fungicides, analysis of fitness‐related variables (mycelial growth, sporulation, germination, and virulence) showed no difference between the groups of S and LS isolates, but a large variation was observed within the group. The LS isolates to thiabendazole that showed a mutation (F200Y) in the β‐tubulin gene did not have fitness penalties. Our results allow a better understanding of the sensitivity and fitness of isolates of C. musae from Brazil, and demonstrate the importance of periodic monitoring to determine the frequency of LS isolates in populations, aiming at more effective management of anthracnose in banana orchards in Brazil.

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Section: Discussionmentioning

confidence: 99%

Analysis of Colletotrichum musae populations from Brazil reveals the presence of isolates with high competitive ability and reduced sensitivity to postharvest fungicides

et al. 2020

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“…This disease is primarily controlled through the use of fungicides but environmental and food safety concerns severely limit their use. Furthermore, several fungicide-resistant isolates of B. dothidea have been frequently reported in recent years as a result of the long-term use of pesticides ( An et al, 2016 ; dos Santos et al, 2019 ; Wang L. et al, 2021 ). Therefore, alternative and environmentally friendly methods of controlling this disease are urgently needed.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of Bacillus velezensis Strain P2-1 for Biocontrol of Apple Postharvest Decay Caused by Botryosphaeria dothidea

et al. 2022

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Botryosphaeria dothidea causes apple ring rot, which is among the most prevalent postharvest diseases of apples and causes significant economic loss during storage. In this study, we investigated the biocontrol activity and possible mechanism of Bacillus velezensis strain P2-1 isolated from apple branches against B. dothidea in postharvest apple fruit. The results showed strain P2-1, one of the 80 different endophytic bacterial strains from apple branches, exhibited strong inhibitory effects against B. dothidea growth and resulted in hyphal deformity. B. velezensis P2-1 treatment significantly reduced the ring rot caused by B. dothidea. Additionally, the supernatant of strain P2-1 exhibited antifungal activity against B. dothidea. Re-isolation assay indicated the capability of strain P2-1 to colonize and survive in apple fruit. PCR and qRT-PCR assays revealed that strain P2-1 harbored the gene clusters required for biosynthesis of antifungal lipopeptides and polyketides. Strain P2-1 treatment significantly enhanced the expression levels of pathogenesis-related genes (MdPR1 and MdPR5) but did not significantly affect apple fruit qualities (measured in fruit firmness, titratable acid, ascorbic acid, and soluble sugar). Thus, our results suggest that B. velezensis strain P2-1 is a biocontrol agent against B. dothidea-induced apple postharvest decay. It acts partially by inhibiting mycelial growth of B. dothidea, secreting antifungal substances, and inducing apple defense responses.

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Strategies for managing fungicide resistance in the Brazilian tropical agroecosystem: Safeguarding food safety, health, and the environmental quality

Ceresini,

Silva,

Vicentini

et al. 2024

Trop. plant pathol.

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Fungicide resistance is an alarming challenge for the Brazilian tropical agricultural systems, with major implications for food safety, human and animal health, as well as for the environment. This review explores strategies to address fungicide resistance within the Brazilian agroecosystem context. We examined historical and current scenarios of fungicide resistance in the Brazilian agroecosystems and the approaches to delay the emergence and mitigate the selection of resistant variants. Our review indicates that the prevalence of resistance in field populations of key plant pathogens in Brazil was due to failures in the implementation of preventive measures. To address this issue, alternative evolutionary-smart strategies against fungicide resistance are proposed, emphasizing institutional actions and public policies. Crucial steps involve strengthening national networks for large-scale foliar and seed fungicide efficacy testing and resistance monitoring, as well as imposing tighter restrictions on the labeling of high-risk single-active formulations. Additionally, the integration of non-chemical disease management strategies and the establishment of a centralized database and information system on fungicide resistance in Brazil are identified as essential for effective resistance monitoring and informed decision-making. To enhance fungicide resistance management, the adoption of a warning system (e.g., based on aerobiology- or on weather-monitoring) for predicting disease epidemics and minimizing fungicide applications is recommended. Increased funding, collaboration, mandatory reporting, and capacity building are required to overcome these challenges. In addition, promoting integrated disease management approaches is vital. By implementing these tailored strategies, Brazil can actively contribute to safeguarding its food safety, protecting human and animal health, and preserving the delicate balance of its unique agroecosystem. The adoption of evolutionary-smart strategies against fungicide resistance will prolong fungicide efficacy, reduce economic costs, and minimize environmental impacts, ensuring sustainable and resilient agriculture in Brazil.

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Sensitivity to methyl benzimidazole carbamate fungicides of Botryosphaeriaceae species from mango orchards in the Northeast of Brazil

Cited by 9 publications

References 36 publications

Analysis of Colletotrichum musae populations from Brazil reveals the presence of isolates with high competitive ability and reduced sensitivity to postharvest fungicides

Analysis of Colletotrichum musae populations from Brazil reveals the presence of isolates with high competitive ability and reduced sensitivity to postharvest fungicides

Isolation and Characterization of Bacillus velezensis Strain P2-1 for Biocontrol of Apple Postharvest Decay Caused by Botryosphaeria dothidea

Strategies for managing fungicide resistance in the Brazilian tropical agroecosystem: Safeguarding food safety, health, and the environmental quality

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