2016
DOI: 10.18632/aging.100958
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Abstract: Differences in the way human and mouse fibroblasts experience senescence in culture had long puzzled researchers. While senescence of human cells is mediated by telomere shortening, Parrinello et al. demonstrated that senescence of mouse cells is caused by extreme oxygen sensitivity. It was hypothesized that the striking difference in oxygen sensitivity between mouse and human cells explains their different rates of aging. To test if this hypothesis is broadly applicable, we cultured cells from 16 rodent speci… Show more

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Cited by 9 publications
(7 citation statements)
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References 28 publications
(25 reference statements)
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“…Notably, this sensitivity appears specific to laboratory rodent strains and is not observed in wild animals of the same species (448). This could reflect the longer telomeres observed in laboratory species 232…”
Section: Marrow-derived Stem Cellsmentioning
confidence: 92%
“…Notably, this sensitivity appears specific to laboratory rodent strains and is not observed in wild animals of the same species (448). This could reflect the longer telomeres observed in laboratory species 232…”
Section: Marrow-derived Stem Cellsmentioning
confidence: 92%
“…Although fibroblasts from some mammals are insensitive to oxygen levels 31 , cells from other species (including humans) exhibit impaired proliferative capacity at ambient O 2 . To better approximate in vivo oxygen concentrations, we assayed cells under physiological O 2 levels and asked whether reduced oxygen affected proliferative capacity in adult fibroblasts from Acomys , Mus , Rattus , and Oryctolagus (Fig.…”
Section: Resultsmentioning
confidence: 99%
“…Amino acid misincorporation at these positions results in reactivation of the luciferase gene and is scored using a luminometer. Primary, low passage, rodent fibroblasts from 17 species with diverse lifespans from our collection (Seluanov et al ., , ; Patrick et al ., ) were cotransfected with the luciferase reporters and Renilla luciferase as a transfection control. Three independent (derived from different animals) cell lines were assayed for each species, and each cell line was tested in triplicate.…”
Section: Resultsmentioning
confidence: 99%