Sensitivity ofListeria monocytogenesto lysozyme predicts ability to proliferate in bovine caruncular epithelial cells

Abstract

22Listeria monocytogenes is an important foodborne pathogen in human and 23 veterinary health, causing significant morbidity and mortality including abortion. 24It has a particular tropism for the gravid uterus, however, the route of infection in 25 reproductive tissues of ruminants (i.e. placentome), is much less clear. In this 26 study, we aimed to investigate a bovine caruncular epithelial cell (BCEC) line as a 27 model for L. monocytogenes infection of the bovine reproductive tract. The BCEC 28 infection model was used to assess the ability of 14 different L. monocytogenes 29 isolates to infect these cells. Lysozyme sensitivity and bacterial survival in 580 µg 30 lysozyme/ml correlated with attenuated ability to proliferate in BCEC (p=0.004 31 and p=0.02, respectively). Four isolates were significantly attenuated compared 32 to the control strain 10403S. One of these strains (AR008) showed evidence of 33 compromised cell wall leading to increased sensitivity to ß-lactam antibiotics, and 34 another (7644) had compromised cell membrane integrity leading to increased 35 sensitivity to cationic peptides. Whole genome sequencing followed by Multi Locus 36Sequence Type analysis identified that five invasive isolates had the same 37 sequence type, ST59, despite originating from three different clinical conditions. 38Virulence gene analysis showed that the attenuated isolate LM4 was lacking two 39 virulence genes (uhpT, virR) known to be involved in intracellular growth and 40 virulence. 41In conclusion, the BCEC model was able to differentiate between the infective 42 potential of different isolates. Moreover, resistance to lysozyme correlated with 43 the ability to invade and replicate within BCEC, suggesting co-selection for 44 surviving challenging environments as the abomasum. 45 3