Sensitive cell apoptosis assay based on caspase-3 activity detection with graphene oxide-assisted electrochemical signal amplification

Chen, Hongxia; Zhang, Jiangjiang; Gao, Yanmin; Liu, Siyu; Koh, Kwangnak; Zhu, Xiaoli; Yin, Yuan

doi:10.1016/j.bios.2015.02.007

Cited by 62 publications

(31 citation statements)

References 33 publications

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“…Since glutaraldehyde may lead to excessive crosslinking of proteins, EDC/NHS chemistry-mediated amidation and click-chemistry (coppercatalyzed azide-alkyne cycloaddition) can be a more effective approach to conjugate proteins. 26,[33][34][35] Instead of using entire protein, using a specic peptide as a subunit antigen to conjugate with AuNPs is an alternative and effective approach for developing vaccines. 36,37 By simply adding an extra cysteine to a peptide, the high affinity between the sulydryl group and gold can ensure a maximal conjugation.…”

Section: Protein/peptide-modied Aunps As Vaccinesmentioning

confidence: 99%

Surface chemistry of gold nanoparticles for health-related applications

2020

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Section: Protein/peptide-modied Aunps As Vaccinesmentioning

confidence: 99%

Surface chemistry of gold nanoparticles for health-related applications

2020

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“…The authors described an efficient, label-free and highly sensitive electrochemical method for simple apoptosis assays. 122 In this approach an acetylated peptide (Ac-GGHDEVD-HGGGC) was immobilized onto the gold electrode surface via a covalent Au-Cys bond to obtain 69 . In the presence of caspase-3, the substrate 69 was hydrolyzed, which resulted in formation of two peptides: the NH 2 -HGGGC fragment with a free N-terminal amino group was then covalently conjugated with GO.…”

Section: Caspase Substratesmentioning

confidence: 99%

Small Molecule Active Site Directed Tools for Studying Human Caspases

et al. 2015

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Caspases are proteases of clan CD and were described for the first time more than two decades ago. They play critical roles in the control of regulated cell death pathways including apoptosis and inflammation. Due to their involvement in the development of various diseases like cancer, neurodegenerative diseases or autoimmune disorders, caspases have been intensively investigated as potential drug targets, both in academic and industrial laboratories. This review presents a thorough, deep, and systematic assessment of all technologies developed over the years for the investigation of caspase activity and specificity using substrates and inhibitors, as well as activity based probes, which in recent years have attracted considerable interest due to their usefulness in the investigation of biological functions of this family of enzymes.

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“…This methodology has unique advantages in the field of medical immunology and will be very useful in the study of T-cellrelated immune responses. 37,38…”

Section: Cd8-fitcmentioning

confidence: 99%

A novel absorption spectrometric method, based on graphene nanomaterials, for detection of hepatocellular carcinoma-specific T lymphocyte cells

Zhu

et al. 2018

IJN

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IntroductionDetection of antigen-specific cytotoxic T lymphocytes (CTLs) is the foundation for understanding hepatocellular carcinoma immune pathology and hepatocellular carcinoma immunotherapy. However, the classical method for labeling CTLs, major histocompatibility complex (MHC)–peptide tetramer, has drawbacks and needs further improvement.Materials and methodsHere, as a new detection probe, a graphene-based MHC–peptide multimer was developed for sensitively and selectively identifying hepatocellular carcinoma-specific T-cells. To assess its detection efficiency, reduced graphene oxide (RGO) was functionalized with hemin and streptavidin to prepare a functionalized HRGO–streptavidin complex. Biotinylated MHC–peptide monomer was subsequently constructed onto HRGO to generate a detection probe for CTL labeling. The number of T-cells was detected through the reaction between HRGO and tetramethylbenzidine.ResultsUsing HRGO/MHC–peptide multimers, the number of T-cells was efficiently detected in both the induction system in vitro and in peripheral blood of patients.ConclusionHRGO/MHC-peptide multimers methodology has application prospects in the detection of antigen peptide-specific T cells.

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Sensitive cell apoptosis assay based on caspase-3 activity detection with graphene oxide-assisted electrochemical signal amplification

Cited by 62 publications

References 33 publications

Surface chemistry of gold nanoparticles for health-related applications

Surface chemistry of gold nanoparticles for health-related applications

Small Molecule Active Site Directed Tools for Studying Human Caspases

A novel absorption spectrometric method, based on graphene nanomaterials, for detection of hepatocellular carcinoma-specific T lymphocyte cells

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