Sensitive biosensor for p53 DNA sequence based on the photothermal effect of gold nanoparticles and the signal amplification of locked nucleic acid functionalized DNA walkers using a thermometer as readout

Tao, Yingzhou; Wang, Weijia; Chen, Fu; Luo, Fang; Guo, Longhua; Qiu, Bin; Lin, Zhenyu

doi:10.1016/j.talanta.2020.121398

Cited by 25 publications

(15 citation statements)

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“…Tao and his colleagues designed a DNA walker biosensor for p53 sensing and applied a thermometer as the readout (Figure 4c ). [ 78 ] Similarly, the DNA walker was anchored on the surface of AuNP, locked nucleic acid (LNA) was functionalized on the end of DNA walker. Once the p53 was introduced, it could hybridize with the LNA and simultaneously initiate the startup of DNA walkers.…”

Section: Number Of Legs For Dna Walkersmentioning

confidence: 99%

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DNA Walkers for Biosensing Development

et al. 2022

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The ability to design nanostructures with arbitrary shapes and controllable motions has made DNA nanomaterials used widely to construct diverse nanomachines with various structures and functions. The DNA nanostructures exhibit excellent properties, including programmability, stability, biocompatibility, and can be modified with different functional groups. Among these nanoscale architectures, DNA walker is one of the most popular nanodevices with ingenious design and flexible function. In the past several years, DNA walkers have made amazing progress ranging from structural design to biological applications including constructing biosensors for the detection of cancer‐associated biomarkers. In this review, the key driving forces of DNA walkers are first summarized. Then, the DNA walkers with different numbers of legs are introduced. Furthermore, the biosensing applications of DNA walkers including the detection‐ of nucleic acids, proteins, ions, and bacteria are summarized. Finally, the new frontiers and opportunities for developing DNA walker‐based biosensors are discussed.

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Section: Number Of Legs For Dna Walkersmentioning

confidence: 99%

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confidence: 99%

DNA Walkers for Biosensing Development

et al. 2022

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“…Moreover, nanoparticle-based LNA biosensors have also been explored. For example, Lin et al reported a photothermal (PT) biosensor based on LNA, DNA walker, and AuNPs [ 143 ]. In their design, the target sequence activated the DNA walkers, leading to the formation of enzyme recognition site via the hybridization of walker strand and substrate strand.…”

Section: Chemically Modified Nucleic Acid Analoguesmentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

Nucleic Acids and Their Analogues for Biomedical Applications

Wang

Chu

et al. 2022

Biosensors

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Nucleic acids are emerging as powerful and functional biomaterials due to their molecular recognition ability, programmability, and ease of synthesis and chemical modification. Various types of nucleic acids have been used as gene regulation tools or therapeutic agents for the treatment of human diseases with genetic disorders. Nucleic acids can also be used to develop sensing platforms for detecting ions, small molecules, proteins, and cells. Their performance can be improved through integration with other organic or inorganic nanomaterials. To further enhance their biological properties, various chemically modified nucleic acid analogues can be generated by modifying their phosphodiester backbone, sugar moiety, nucleobase, or combined sites. Alternatively, using nucleic acids as building blocks for self-assembly of highly ordered nanostructures would enhance their biological stability and cellular uptake efficiency. In this review, we will focus on the development and biomedical applications of structural and functional natural nucleic acids, as well as the chemically modified nucleic acid analogues over the past ten years. The recent progress in the development of functional nanomaterials based on self-assembled DNA-based platforms for gene regulation, biosensing, drug delivery, and therapy will also be presented. We will then summarize with a discussion on the advanced development of nucleic acid research, highlight some of the challenges faced and propose suggestions for further improvement.

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“…As an important kind of DNA machine, the DNA walker has attracted extensive attention in biosensor design in the past several years. [ 18‐19 ] A typical DNA walker mainly consists of three elements, including a walking strand, a track, and a driving motor. [ 20‐22 ] The DNA walking process is typically activated by the reaction of strand hybridization, enzymatic reaction (including nicking endonuclease, exonuclease, and DNAzyme), or environmental stimuli such as light irradiation.…”

Section: Background and Originality Contentmentioning

confidence: 99%

Target Extension‐Activated DNA Walker on Nanoparticles for Digital Counting‐Based Analysis of MicroRNA^†

et al. 2021

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RNA recognition | DNA cleavage | Digital counting | Total internal reflection fluorescent microscope | NanoparticlesMicroRNAs (miRNAs), especially exosomal miRNAs, are promising noninvasive biomarkers in early-stage cancer diagnosis and disease treatment monitoring. However, their precise and sensitive quantification remains challenging due to their small size and low abundance. Herein, we have developed a nanoparticle-confined DNA walker strategy for the specific detection of miRNA. In the existence of the target miRNA, the on-particle DNA walking reaction will be initiated, providing a fluorescence-positive nanoparticle. Otherwise, the nanoparticle would be fluorescence-negative. Utilizing the total internal reflection fluorescent microscope (TIRFM) to digitally count the fluorescence-positive nanoparticles, the proposed method possesses a detection limit of 0.2 pmol/L miRNA and can accurately distinguish the single-base mismatched target. This design combines the merits of the DNA walker for signal amplification and the TIRFM for highly sensitive detection, paving a new way for the digital counting-based analysis of exosomal miRNAs.

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Sensitive biosensor for p53 DNA sequence based on the photothermal effect of gold nanoparticles and the signal amplification of locked nucleic acid functionalized DNA walkers using a thermometer as readout

Cited by 25 publications

References 37 publications

DNA Walkers for Biosensing Development

DNA Walkers for Biosensing Development

Nucleic Acids and Their Analogues for Biomedical Applications

Target Extension‐Activated DNA Walker on Nanoparticles for Digital Counting‐Based Analysis of MicroRNA^†

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Sensitive biosensor for p53 DNA sequence based on the photothermal effect of gold nanoparticles and the signal amplification of locked nucleic acid functionalized DNA walkers using a thermometer as readout

Cited by 25 publications

References 37 publications

DNA Walkers for Biosensing Development

DNA Walkers for Biosensing Development

Nucleic Acids and Their Analogues for Biomedical Applications

Target Extension‐Activated DNA Walker on Nanoparticles for Digital Counting‐Based Analysis of MicroRNA†

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Product

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Target Extension‐Activated DNA Walker on Nanoparticles for Digital Counting‐Based Analysis of MicroRNA^†