Sensing of biomolecular interactions using fluorescence complementing systems in living cells

Zhang, Xian-En; Cui, Zongqiang; Wang, Dianbing

doi:10.1016/j.bios.2015.07.069

Cited by 31 publications

(19 citation statements)

References 75 publications

(75 reference statements)

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“…Bimolecular fluorescence complementation (Fig F) is based on the division of a fluorescent protein (e.g., YFP) into two distinct non‐fluorescent fragments, which are then fused to “bait” and “prey” proteins of interest. Interaction between bait and prey allows the two non‐fluorescent fragments to associate and form a fluorescent complex, which can be viewed by microscopy or flow cytometry (Kerppola, ; Zhang et al , ).…”

Section: Guide To Available Methodsmentioning

confidence: 99%

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Fundamentals of protein interaction network mapping

Snider

Kotlyar

Saraon

et al. 2015

Molecular Systems Biology

242

184

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Studying protein interaction networks of all proteins in an organism (“interactomes”) remains one of the major challenges in modern biomedicine. Such information is crucial to understanding cellular pathways and developing effective therapies for the treatment of human diseases. Over the past two decades, diverse biochemical, genetic, and cell biological methods have been developed to map interactomes. In this review, we highlight basic principles of interactome mapping. Specifically, we discuss the strengths and weaknesses of individual assays, how to select a method appropriate for the problem being studied, and provide general guidelines for carrying out the necessary follow‐up analyses. In addition, we discuss computational methods to predict, map, and visualize interactomes, and provide a summary of some of the most important interactome resources. We hope that this review serves as both a useful overview of the field and a guide to help more scientists actively employ these powerful approaches in their research.

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Section: Guide To Available Methodsmentioning

confidence: 99%

“…Different fluorescent proteins can also be used in combination, allowing the visualization of multiple PPIs in parallel in single cells. The method is best suited for detecting binary interactions (Hu et al , ; Kerppola, ; Zhang et al , ).…”

Section: Guide To Available Methodsmentioning

confidence: 99%

Fundamentals of protein interaction network mapping

Snider

Kotlyar

Saraon

et al. 2015

Molecular Systems Biology

242

184

View full text Add to dashboard Cite

show abstract

“…For the inding of solitary-bacterium in fewer periods established through the plate-counting technique then understood through spending two selfgoverning visual methods. Additionally, we were capable of spotting multiple microbial sections through from top to bottom amount by using a 384well microplate system (Xie et al, 2007;Zhang et al, 2016) for food and water shield essential aimed at precise methods for pathogenic micro-organisms documentation and inding to permit the anticipation then organization of pathogenic syndromes.…”

Section: Nanoparticle-based Methodsmentioning

confidence: 99%

Host pathogen interaction and pathogen detection methods

Satpathy¹,

Manikandan²

2020

ijrps

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Identification and detection of pathogens are the strategies towards the elucidation of infectious disease. There are growth and significance in the field of Nanomedicine. Present circumstance here is a need in research surrounding the topic of nanoparticle (NP) properties, mainly concerned with mechanisms of action with the bacterial cell. Metal NPs are now showing strong antibacterial activity. The bacterial cells have more resistance towards the antibiotics, which has met the research community to innovate novel antibiotic agents. The ability to detect micro-organisms has become an essential requirement in food-processing technology for clinical and environmental monitoring. In keeping with traditional methods of detection, those are time taking and develop the result solitary has to wait for long hours. Nowadays, there are many rapid and sensitive methods developed by the effort of researchers and inventors. Nanoparticles act as the alternative to pathogen detection. The size, shape and morphology of nanoparticles play a role for the pathogen nanoparticle communication. Here, in this review article, we discuss the nanoparticle and bacterial cell interaction. The different methods based on culture-based, optical sensing-based and nanoparticle-based methods all discussed in this chapter.

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“…2) with another posi tions of the split site that exhibited lower contrast in vivo in comparison with iSplit [110]. Re assembly of iSplit and iRFP713 BiFC reporter is irreversible, which is typi cal for the majority of PPI reporters based on the GFP like proteins [104].…”

Section: Application Of Nir Fluorescent Biomarkers Reporters and Bimentioning

confidence: 99%

Near-Infrared Fluorescent Proteins and Their Applications

Karasev

Stepanenko

Rumyantsev

et al. 2019

Biochemistry Moscow

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High transparency, low light scattering, and low autofluorescence of mammalian tissues in the near infrared (NIR) spectral range (~650 900 nm) open a possibility for in vivo imaging of biological processes at the micro and macroscales to address basic and applied problems in biology and biomedicine. Recently, probes that absorb and fluoresce in the NIR optical range have been engineered using bacterial phytochromes-natural NIR light absorbing photoreceptors that regulate metabolism in bacteria. Since the chromophore in all these proteins is biliverdin, a natural product of heme catabolism in mammalian cells, they can be used as genetically encoded fluorescent probes, similarly to GFP like fluores cent proteins. In this review, we discuss photophysical and biochemical properties of NIR fluorescent proteins, reporters, and biosensors and analyze their characteristics required for expression of these molecules in mammalian cells. Structural features and molecular engineering of NIR fluorescent probes are discussed. Applications of NIR fluorescent proteins and biosensors for studies of molecular processes in cells, as well as for tissue and organ visualization in whole body imaging in vivo, are described. We specifically focus on the use of NIR fluorescent probes in advanced imaging technologies that com bine fluorescence and bioluminescence methods with photoacoustic tomography.

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Sensing of biomolecular interactions using fluorescence complementing systems in living cells

Cited by 31 publications

References 75 publications

Fundamentals of protein interaction network mapping

Fundamentals of protein interaction network mapping

Host pathogen interaction and pathogen detection methods

Near-Infrared Fluorescent Proteins and Their Applications

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