Self-contained microfluidic systems: a review

Boyd‐Moss, Mitchell; Baratchi, Sara; Venere, Martina Di; Khoshmanesh, Khashayar

doi:10.1039/c6lc00712k

Cited by 120 publications

(96 citation statements)

References 120 publications

(118 reference statements)

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“…While the label-free characteristic of DEP manipulation has traditionally enabled sample preparation for detection of molecular nucleic-acid type biomarkers by enhancing capture efficiency and selectivity for impedance-based detection, current trends are directed towards the application of DEP within miniaturized point-of-care type microbial concentration 225,226 and sensing devices 227 . A prime example of this is the commercialization of the DEPIM technology by Panasonic Healthcare in the form of a microbial counter for dental applications.…”

Section: Challenges and Emerging Needsmentioning

confidence: 99%

Review: Microbial analysis in dielectrophoretic microfluidic systems

Fernandez

Rohani

Farmehini

et al. 2017

Analytica Chimica Acta

122

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Infections caused by various known and emerging pathogenic microorganisms, including antibiotic-resistant strains, are a major threat to global health and well-being. This highlights the urgent need for detection systems for microbial identification, quantification and characterization towards assessing infections, prescribing therapies and understanding the dynamic cellular modifications. Current state-of-the-art microbial detection systems exhibit a trade-off between sensitivity and assay time, which could be alleviated by selective and label-free microbial capture onto the sensor surface from dilute samples. AC electrokinetic methods, such as dielectrophoresis, enable frequency-selective capture of viable microbial cells and spores due to polarization based on their distinguishing size, shape and sub-cellular compositional characteristics, for downstream coupling to various detection modalities. Following elucidation of the polarization mechanisms that distinguish bacterial cells from each other, as well as from mammalian cells, this review compares the microfluidic platforms for dielectrophoretic manipulation of microbials and their coupling to various detection modalities, including immuno-capture, impedance measurement, Raman spectroscopy and nucleic acid amplification methods, as well as for phenotypic assessment of microbial viability and antibiotic susceptibility. Based on the urgent need within point-of-care diagnostics towards reducing assay times and enhancing capture of the target organism, as well as the emerging interest in isolating intact microbials based on their phenotype and subcellular features, we envision widespread adoption of these label-free and selective electrokinetic techniques.

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Section: Challenges and Emerging Needsmentioning

confidence: 99%

Review: Microbial analysis in dielectrophoretic microfluidic systems

Fernandez

Rohani

Farmehini

et al. 2017

Analytica Chimica Acta

122

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“…Connecting the frame with two reservoirs, using microfluidic valves as the ones that have been demonstrated recently [17], the film would behave as an optofluidic pump. Since it vibrates at resonance, the optical power could be very low which may be a crucial issue in biological systems where optical power is detrimental to cells in general [18].…”

Section: Discussionmentioning

confidence: 99%

Towards an optofluidic pump?

Émile¹,

Émile²

2016

Optofluidics, Microfluidics and Nanofluidics

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Most of the vibrating mechanisms of optofluidicsystems are based on local heating of membranes that in-duces liquid flow. We report here a new type of diaphragmpump in a liquid film based on the optical radiation pres-sure force. We modulate a low power laser that gener-ates, at resonance, a symmetric vibration of a free standingsoap film. The film lifetime strongly varies from 56 s at lowpower (2 mW) to 2 s at higher power (70 mW). Since thelaser beam only acts mechanically on the interfaces, sucha pump could be easily implemented on delicate micro-equipment on chips or in biological systems

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“…An attractive characteristic of microfluidics is portability, which is amenable to point-of-care applications, and substantial ongoing efforts focus on developing portable diagnostic microfluidic devices with easily accessible detection like smartphone enabled sensing [60]. Additionally, the supporting instrumentation for microfluidics including electrical, optical and data analysis systems should be integrated within the device or miniaturized to facilitate point-of-care diagnosis [61–63]. …”

Section: Conclusion and Future Trendsmentioning

confidence: 99%

Recent advances in microfluidic sample preparation and separation techniques for molecular biomarker analysis: A critical review

Sonker

Sahore

Woolley

2017

Analytica Chimica Acta

138

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Microfluidics is a vibrant and expanding field that has the potential for solving many analytical challenges. Microfluidics show promise to provide rapid, inexpensive, efficient, and portable diagnostic solutions that can be used in resource-limited settings. Researchers have recently reported various microfluidic platforms for biomarker analysis applications. Sample preparation processes like purification, preconcentration and labeling have been characterized on-chip. Additionally, improvements in microfluidic separation techniques have been reported for cellular and molecular biomarkers. This review critically evaluates microfluidic sample preparation platforms and separation methods for biomarker analysis reported in the last two years. Key advances in device operation and ability to process different sample matrices in a variety of device materials are highlighted. Finally, current needs and potential future directions for microfluidic device development to realize its full diagnostic potential are discussed.

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Self-contained microfluidic systems: a review

Cited by 120 publications

References 120 publications

Review: Microbial analysis in dielectrophoretic microfluidic systems

Review: Microbial analysis in dielectrophoretic microfluidic systems

Towards an optofluidic pump?

Recent advances in microfluidic sample preparation and separation techniques for molecular biomarker analysis: A critical review

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