Self-assembly of thiolipid molecular layers on gold surfaces: optical and electrochemical characterization

Lang, Holger; Duschl, Claus; Grätzel, Michaël; Vogel, Horst

doi:10.1016/0040-6090(92)90413-6

Cited by 72 publications

(55 citation statements)

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“…9 The most common approach employed for the surface immobilisation of phospholipids entails the formation of a surface-supported lipid bilayer. 10 These are typically prepared using surface-bound alkylsilane, 11,12 alkanethiol, 7,13,14,15 or thiolipid 10,16 self-assembled monolayers (SAMs). The inherent well-packed nature of such SAMs enables the formation of a non-interdigitated hybrid lipid bilayer comprising a layer of phospholipids on top of a layer of surface bound SAM alkyl chain molecules.…”

Section: Introductionmentioning

confidence: 99%

Combining plasmachemical emulsion-templating with ATRP to create macroporous lipophilic surfaces

Brown

Wood

Badyal

2014

Journal of Colloid and Interface Science

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Publisher's copyright statement: NOTICE: this is the author's version of a work that was accepted for publication in Journal of Colloid and Interface Science. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be re ected in this document. Changes may have been made to this work since it was submitted for publication. A de nitive version was subsequently published in Journal of Colloid and Interface Science, 421, 2014, 10.1016/j.jcis.2014.01.019. Use policyThe full-text may be used and/or reproduced, and given to third parties in any format or medium, without prior permission or charge, for personal research or study, educational, or not-for-pro t purposes provided that:• a full bibliographic reference is made to the original source • a link is made to the metadata record in DRO • the full-text is not changed in any way The full-text must not be sold in any format or medium without the formal permission of the copyright holders.Please consult the full DRO policy for further details. ABSTRACTWell-defined alkyl chain side group polymer brushes have been tethered onto high surface area macroporous pulsed plasmachemical emulsion-templated poly(vinylbenzyl chloride) initiator layers (typically 600-700 m 2 g -1 ) using atom transfer radical polymerisation (ATRP). Immobilisation of phospholipids onto these bioactive surfaces is found to occur through interdigitation, and the efficacy of lipid binding is governed by the alkyl side group chain length of the polymer brushes.

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Section: Introductionmentioning

confidence: 99%

Combining plasmachemical emulsion-templating with ATRP to create macroporous lipophilic surfaces

Brown

Wood

Badyal

2014

Journal of Colloid and Interface Science

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“…calculated with constant refractive index, the "adlayer thickness" becomes a measure of molecule density; only at close packing it can be interpreted in terms of molecular dimensions. According to published data obtained with well-defined model systems, n = 1.45 is a reasonable value for the refractive index of lipids in a fluid state and adsorbed proteins (Lang et al, 1992(Lang et al, , 1994Spinke et al, 1992Spinke et al, , 1993Reiter et al, 1993; Terrettaz et ai., 1993) (and references therein).5 For lipids and fatty acid salts in a crystalline state, refractive indices in the range of n = 1.5 seem more appropriate (Swalen, 1986;Kooyman & Krull, 1991;Schmidt et al, 1992). But in our experiments, a crystalline state of lipids on the waveguide can be excluded (see below).…”

Section: Choice Of Refractive Index For Optical Measurementsmentioning

confidence: 84%

“…After thiolipid binding to the waveguides, lipid bilayers of POPC, of POPC/biotin-DPPE, or of POPC/lipopeptide in selected molar ratios were formed by vesicle spreading (Kalb et al, 1992;Lang et al, 1992Lang et al, , 1994Terrettaz et al, 1993) or by dilution of mixed micelle solutions (Lang et al, 1994). Vesicle dispersions were produced by first drying down a chloroform solution of 1 mg either of POPC or a mixture of POPC and biotin-DPPE.…”

Section: Formation Of the Lipid Bilayer And Protein Bindingmentioning

confidence: 99%

“…Strategies for the covalent attachment of lipid layers on optical devices have been already realized in the case of gold surfaces for SPR measurements using disulfide-bearing phospholipids (Lang et al, 1992(Lang et al, , 1994 and disulfide-functionalized amphiphilic copolymers (Erdelen et al, 1994).…”

mentioning

confidence: 99%

“…This procedure is simple, fast, and reproducible. Thiolipids with a polar spacer between phosphate and sulfur group allow for a further decoupling of the lipid layer from the solid support and provide an aqueous environment on both sides of the bilayer that is sufficiently large to accommodate the extramembranous parts of a membrane-traversing protein; this concept was realized previously on gold surfaces (Lang et al, 1992(Lang et al, , 1993(Lang et al, , 1994. Although chemical crosslinkers have been used for tethering thiol-bearing proteins and oligonucleotides to various supports (Hong et al, 1994;Lee et al, 1994), to our knowledge, no attempts have been made so far to anchor lipid bilayers to hydrophilic supports using maleimide chemistry.…”

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confidence: 99%

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Covalent attachment of functionalized lipid bilayers to planar waveguides for measuring protein binding to biomimetic membranes

et al. 1995

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A new method is presented for measuring sensitively the interactions between ligands and their membrane-bound receptors in situ using integrated optics, thus avoiding the need for additional labels. Phospholipid bilayers were attached covalently to waveguides by a novel protocol, which can in principle be used with any glass-like surface.In a first step, phospholipids carrying head-group thiols were covalently immobilized onto Si02-Ti02 waveguide surfaces. This was accomplished by acylation of aminated waveguides with the heterobifunctional crosslinker N-succinimidyl-3-maleimidopropionate, followed by the formation of thioethers between the surface-grafted maleimides and the synthetic thiolipids. The surface-attached thiolipids served as hydrophobic templates and anchors for the deposition of a complete lipid bilayer either by fusion of lipid vesicles or by lipid self-assembly from mixed lipid/detergent micelles. The step-by-step lipid bilayer formation on the waveguide surface was monitored in situ by an integrated optics technique, allowing the simultaneous determination of optical thickness and one of the two refractive indices of the adsorbed organic layers. Surface coverages of 50-60% were calculated for thiolipid layers. Subsequent deposition of POPC resulted in an overall lipid layer thickness of 45-50 A , which corresponds to the thickness of a fluid bilayer membrane.Specific recognition reactions occurring at cell membrane surfaces were modeled by the incorporation of lipidanchored receptor molecules into the supported bilayer membranes. (1) The outer POPC layer was doped with biotinylated phosphatidylethanolamine. Subsequent specific binding of streptavidin was optically monitored. (2) A lipopeptide was incorporated in the outer POPC monolayer. Membrane binding of monoclonal antibodies, which were directed against the peptide moiety of the lipopeptide, was optically detected. The specific antibody binding correlated well with the lipopeptide concentration in the outer monolayer.

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Biosensors Based on Thin Lipid Films and Liposomes

Nikolelis¹,

Hianik

Krull³

1999

Electroanalysis

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This article reports the theory and analytical applications of thin lipid ®lms. Recent advances of electrochemical devices based on lipid membranes have lead to reports of construction of biosensors for environmental and food applications, and may provide opportunities for commercial fabrication. The methods of formation of lipid membranes on various supports including metals (silver, gold, stainless steel), agar, conducting polymers and ultra®ltration membranes have provided stabilization of lipid ®lms with a diversity of analytical applications in real samples. Methods of immobilization and incorporation of various functional macromolecules are summarized. Several examples of the application of various methods for study of physical properties of supported bilayer lipid membranes are described. Applications of lipid-based biosensors in analytical chemistry for determination of compounds are demonstrated, including a diversity of chemical compounds such as environmental pollutants (ammonia and carbon dioxide, cyanide ions, etc.) and food toxins (a¯atoxin M 1 and direct detection of toxin in real samples such as milk and milk preparations). Methods for application of liposomes as a sensing system are also summarized.

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Self-assembly of thiolipid molecular layers on gold surfaces: optical and electrochemical characterization

Cited by 72 publications

References 6 publications

Combining plasmachemical emulsion-templating with ATRP to create macroporous lipophilic surfaces

Combining plasmachemical emulsion-templating with ATRP to create macroporous lipophilic surfaces

Covalent attachment of functionalized lipid bilayers to planar waveguides for measuring protein binding to biomimetic membranes

Biosensors Based on Thin Lipid Films and Liposomes

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