Self-assembly of the pre-formed inclusion complexes between cyclodextrins and alkanethiols on gold electrodes

Yan, Juchao; Dong, Shaojun

doi:10.1016/s0022-0728(97)80060-5

Cited by 6 publications

(5 citation statements)

References 36 publications

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“…In general, specific binding of a target molecule requires a recognition entity on the sensor surface. Gold (Au (111)) microcrystalline domains serve this purpose in the case of alkanethiol, ,,, whereas an antibody is used for biological entities. − When the target of interest binds to the cantilever's sensing surface, the effective mass of the cantilever increases which decreases the cantilever's resonant frequency. Hence, by monitoring a cantilever resonant frequency change, quantitative measurements of the cantilever's effective mass change can be measured in real time.…”

Section: Principle Of Measurementmentioning

confidence: 99%

Monitoring of the Self-Assembled Monolayer of 1-Hexadecanethiol on a Gold Surface at Nanomolar Concentration Using a Piezo-Excited Millimeter-Sized Cantilever Sensor

Campbell

Mutharasan

2005

Langmuir

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In this paper, we describe a new method of measuring alkanethiol monolayer formation on a gold surface. A gold-coated millimeter-sized rectangular-shaped lead zirconate titanate (PZT) cantilever of dimensions 3.5 x 2 x 0.05 mm, previously shown to detect a picogram level of mass change, was used to measure the adsorption kinetics of 1-hexadecanethiol in ethanol over six orders of concentration range (1 nM to 10 mM) in real time. The flexural mode of cantilever vibration, 45.5 +/- 0.01 kHz, was monitored during the self-assembly. The total resonant frequency change obtained for the 1 nM, 10 nM, 100 nM, 1 microM, 4 mM, 8 mM, and 10 mM thiol concentrations were 116 +/- 2 (n = 2), 225 (n = 1), 270 +/- 10 (n = 2), 440 +/- 10 (n = 2), 900 +/- 10 (n = 2), 900 +/- 10 (n = 2), and 900 +/- 10 (n = 2) Hz, respectively. These results compare favorably to literature results in that the rate of the monolayer formation is concentration-dependent and the exponential change during adsorption follows the reversible first-order Langmuir kinetic model. The rate constants of adsorption and desorption were 0.061 M(-1) s(-1) and 3.61 x 10(-4) s(-1), respectively. The significance of the results is that millimeter-sized PZT cantilevers can be used in real-time for characterizing self-assembly of monolayer formation at nanomolar concentration levels. In addition, at 1 nM, the adsorption was found not to be diffusion limited.

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Section: Principle Of Measurementmentioning

confidence: 99%

Monitoring of the Self-Assembled Monolayer of 1-Hexadecanethiol on a Gold Surface at Nanomolar Concentration Using a Piezo-Excited Millimeter-Sized Cantilever Sensor

Campbell

Mutharasan

2005

Langmuir

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“…Adsorption of proteins on solid surfaces and the evaluation of their characteristics for comparison to the native protein are of general interest in biomedical − and biomaterials area and in efforts to design biocompatible surfaces. , Protein adsorption on synthetic material is a complex process that involves conformational changes, orientation and rearrangement, and protein detachment, which affects the protein activity. , Therefore, there is a need for a rapid real-time protein adsorption technique to characterize the adsorption process on various surfaces. The well-characterized self-assembled monolayer (SAM) of alkanethiol with different terminal function groups on gold surface is a general model system for studying the adsorption of proteins and has been used by several researchers for biointerface design. − The functional end groups provide proper orientation and molecular organization when SAM is formed on a gold surface.…”

mentioning

confidence: 99%

Use of Piezoelectric-Excited Millimeter-Sized Cantilever Sensors To Measure Albumin Interaction with Self-Assembled Monolayers of Alkanethiols Having Different Functional Headgroups

Campbell

Mutharasan

2006

Anal. Chem.

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In this paper, we describe a new modality of measuring human serum albumin (HSA) adsorption continuously on CH3-, COOH-, and OH-terminated self-assembled monolayers (SAMs) of C11-alkanethiols and the direct quantification of the adsorbed amount. A gold-coated piezoelectric-excited millimeter-sized cantilever (PEMC) sensor of 6-mm2 sensing area was fabricated, where resonant frequency decreases upon mass increase. The resonant frequency in air of the detection peak was 45.5 +/- 0.01 kHz. SAMs of C11-thiols (in absolute ethanol) with different end groups was prepared on the PEMC sensor and then exposed to buffer solution containing HSA at 10 microg/mL. The resonant frequency decreased exponentially and reached a steady-state value within 30 min. The decrease in resonant frequency indicates that the mass of the sensor increased due to HSA adsorption onto the SAM layer. The frequency change obtained for the HSA adsorption on CH3-, COOH-, and OH-terminated SAM were 520.8 +/- 8.6 (n = 3), 290.4 +/- 6.1 (n = 2), and 210.6 +/- 8.1 Hz (n = 3), respectively. These results confirm prior conclusions that albumin adsorption decreased in the order, CH(3) > COOH > OH. Observed binding rate constants were 0.163 +/- 0.003, 0.248 +/- 0.006, and 0.381 +/- 0.001 min(-1), for methyl, carboxylic, and hydroxyl end groups, respectively. The significance of the results reported here is that both the formation of self-assembled monolayers and adsorption of serum protein onto the formed layer can be measured continuously, and quantification of the adsorbed amount can be determined directly.

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“…The resulting n-CD-MHA was used as a loading solution to assemble monolayers. The gold electrode, after being cleaned thoroughly (sonication in absolute ethanol and then in water), 13 was electrocycled between 0.6 V and À0.2 V (vs. SCE) in 2.5 mM K 3 Fe(CN) 6 /K 4 Fe(CN) 6 until a reproducible voltammogram was obtained. The gold electrode (or the QCM oscillators with gold disk, also cleaned thoroughly) was immediately immersed in the loading solution for 16 h at 277.1 K. It was taken out from the solution, rinsed vigorously with purified water and blown dry with pure nitrogen prior to use.…”

Section: 32mentioning

confidence: 99%

“…Since the molecules on the HD-SAM are compactly packed, their conformation will not change even when an external stimulation is applied to them. 13…”

Section: Introductionmentioning

confidence: 99%

Selective assembly of specifically charged proteins on an electrochemically switched surface

et al. 2005

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Self-assembly of the pre-formed inclusion complexes between cyclodextrins and alkanethiols on gold electrodes

Cited by 6 publications

References 36 publications

Monitoring of the Self-Assembled Monolayer of 1-Hexadecanethiol on a Gold Surface at Nanomolar Concentration Using a Piezo-Excited Millimeter-Sized Cantilever Sensor

Monitoring of the Self-Assembled Monolayer of 1-Hexadecanethiol on a Gold Surface at Nanomolar Concentration Using a Piezo-Excited Millimeter-Sized Cantilever Sensor

Use of Piezoelectric-Excited Millimeter-Sized Cantilever Sensors To Measure Albumin Interaction with Self-Assembled Monolayers of Alkanethiols Having Different Functional Headgroups

Selective assembly of specifically charged proteins on an electrochemically switched surface

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