Self-assembly mechanisms of silk protein nanostructures on two-dimensional surfaces

Kurland, Nicholas E.; Kundu, Joydip; Pal, Shilpa; Kundu, Subhas C.; Yadavalli, Vamsi K.

doi:10.1039/c2sm25313e

Cited by 33 publications

(39 citation statements)

References 52 publications

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“…Moreover, in the case of VI and IV, macromolecules created fractal‐like aggregates. During sample preparation, conjugates probably underwent diffusion‐limited aggregation that was observed as fractal‐like patterns formation . However, depending on the number of arms, obtained structures were different.…”

Section: Resultsmentioning

confidence: 99%

Self‐assembling water‐soluble polymethacrylate–MTX conjugates: The significance of macromolecules architecture on drug conjugation efficiency, the final shape of particles, and drug release

Bernaczek

Mielańczyk

et al. 2019

J Biomed Mater Res

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Water‐soluble polymer–methotrexate (MTX) conjugates were obtained via efficient carbodiimide‐mediated amidation (E = 17–100%). Binding abilities between water‐soluble V‐shaped or star‐shaped copolymers and MTX were studied by isothermal titration calorimetry spectroscopic (UV–vis, NMR) and microscopic (scanning electron microscopy and transmission electron microscopy) techniques. The efficiency of the amidation reaction has depended on the amount of pendant amino alcohol groups and zeta potential (ZP) values of polymeric carries. The sizes of aggregates formed by polymer–drug conjugates in water increased with the number of copolymer arms (202–774 nm at 37°C). Moreover, the conjugates with the high amount of bounded MTX molecules (nMTX > 78) exhibited negative ZP values. The drug release experiments revealed that the amount of the released MTX depends on pH and can be controlled via shape, topology, and composition of polymeric carrier. Preliminary cytotoxicity studies of V‐shaped‐MTX conjugate on human immortalized nontumorigenic keratinocyte (HaCaT) cells indicated cytocompatibility of the compound in a wide range of concentrations. The results of our studies have shown that physicochemical and drug release properties of obtained polymer–MTX prodrugs can be tailored via the structure and the topology of the polymeric carrier. Thus conjugates might find the application in a different type of treatment (cancer or psoriasis therapy) and administration (intravenous, dermal, or pulmonary). © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B:2476–2487, 2019.

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Section: Resultsmentioning

confidence: 99%

Self‐assembling water‐soluble polymethacrylate–MTX conjugates: The significance of macromolecules architecture on drug conjugation efficiency, the final shape of particles, and drug release

Bernaczek

Mielańczyk

et al. 2019

J Biomed Mater Res

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“…For isolating sericin in the native condition, the cut cocoons and peduncle pieces are soaked in 1% NaCl solution at room temperature overnight with shaking at 100 rpm followed by precipitation and resolubilization [ 13 – 15 ]. Sericin displays the ability to self-assemble via multiple aggregation mechanisms [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Non-Mulberry and Mulberry Silk Protein Sericins as Potential Media Supplement for Animal Cell Culture

Sahu

Pal

Sapru

et al. 2016

BioMed Research International

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Silk protein sericins, in the recent years, find application in cosmetics and pharmaceuticals and as biomaterials. We investigate the potential of sericin, extracted from both mulberry Bombyx mori and different non-mulberry sources, namely, tropical tasar, Antheraea mylitta; muga, Antheraea assama; and eri, Samia ricini, as growth supplement in serum-free culture medium. Sericin supplemented media containing different concentrations of sericins from the different species are examined for attachment, growth, proliferation, and morphology of fibrosarcoma cells. The optimum sericin supplementation seems to vary with the source of sericins. The results indicate that all the sericins promote the growth of L929 cells in serum-free culture media; however, S. ricini sericin seems to promote better growth of cells amongst other non-mulberry sericins.

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“…72 Unfortunately, the present study can render no further clues to the possible connection between these states. Solution-state dependences of thin film aggregate structures and morphologies had been observed for a wide range of material systems including colloids, 64,73,74 amphiphilic block copolymers, 75 polymer composites, 35 76 In this respect, it seems that much remains to be explored for the case with fullerene solutions and thin films. …”

Section: Structural Features Of C 60 Aggregates In Toluenementioning

confidence: 98%

Mesoscale aggregation properties of C₆₀ in toluene and chlorobenzene

et al. 2016

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The mesoscale aggregation properties of C60 in two distinct aromatic solvents (toluene and chlorobenzene) and a practical range of concentrations (c = 1-2 and c = 1-5 mg mL(-1), respectively) were systematically explored by static/dynamic light scattering (SLS/DLS), small angle X-ray scattering (SAXS), depolarized dynamic light scattering (DDLS), and cryogenic transmission electron microscopy (cryo-TEM) analyses. The central observations were as follows: (1) aggregate species of sizes in the range of several hundred nanometers have been independently revealed by SLS, DLS, and DDLS analyses for both solvent systems. (2) DDLS and cryo-TEM measurements further revealed that while C60 clusters are notably anisotropic (rod-like) in chlorobenzene, they are basically isotropic (spherical) in toluene. (3) Detailed analyses of combined SLS and SAXS profiles suggested that varied, yet self-similar, solvent-induced aggregate units were responsible for the distinct (mesoscale) aggregation features noted above. (4) From a dynamic perspective, specially commissioned DLS measurements ubiquitously displayed two relaxation modes (fast and slow mode), with the second (slow) mode being q (wave vector) independent. While the fast mode in both solvent systems was basically diffusive by nature and leads to geometrical features in good agreement with the above static analyses, the slow mode was analyzed and tentatively suggested to reflect the effect of mutual confinement. (5) Micron-scale aggregate morphology of drop-cast thin films displays similar contrasting features for the two solvent media used. Overall, this study suggests that solvent-induced, nanoscale, aggregate units may be a promising factor to control a hierarchy of microscopic aggregation properties of C60 solutions and thin films.

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Self-assembly mechanisms of silk protein nanostructures on two-dimensional surfaces

Cited by 33 publications

References 52 publications

Self‐assembling water‐soluble polymethacrylate–MTX conjugates: The significance of macromolecules architecture on drug conjugation efficiency, the final shape of particles, and drug release

Self‐assembling water‐soluble polymethacrylate–MTX conjugates: The significance of macromolecules architecture on drug conjugation efficiency, the final shape of particles, and drug release

Non-Mulberry and Mulberry Silk Protein Sericins as Potential Media Supplement for Animal Cell Culture

Mesoscale aggregation properties of C₆₀ in toluene and chlorobenzene

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Self-assembly mechanisms of silk protein nanostructures on two-dimensional surfaces

Cited by 33 publications

References 52 publications

Self‐assembling water‐soluble polymethacrylate–MTX conjugates: The significance of macromolecules architecture on drug conjugation efficiency, the final shape of particles, and drug release

Self‐assembling water‐soluble polymethacrylate–MTX conjugates: The significance of macromolecules architecture on drug conjugation efficiency, the final shape of particles, and drug release

Non-Mulberry and Mulberry Silk Protein Sericins as Potential Media Supplement for Animal Cell Culture

Mesoscale aggregation properties of C60 in toluene and chlorobenzene

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Mesoscale aggregation properties of C₆₀ in toluene and chlorobenzene