Self-Assembly Enhances the Strength of Fibers Made from Vimentin Intermediate Filament Proteins

Pinto, Nicole; Yang, Fei-Chi; Negishi, Atsuko; Rheinstädter, Maikel C.; Gillis, Todd E.; Fudge, Douglas S.

doi:10.1021/bm401600a

Cited by 47 publications

(71 citation statements)

References 31 publications

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“…Starting with either hagfish protein from the slime glands or the related intermediate filament vimentin, produced by recombinant bacteria, the researchers freeze-dried the proteins and dissolved them in acid, then dripped the solution on top of a buffer. It readily formed a film, and when the researchers picked it up with forceps it came up as thread bundles (4,5).…”

Section: Hagfish Inspirationmentioning

confidence: 99%

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Will hagfish yield the fibers of the future?

Dance¹

2016

Proc. Natl. Acad. Sci. U.S.A.

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Section: Hagfish Inspirationmentioning

confidence: 99%

“…Either version, says Rainey, might be useful for imparting flexibility or adding strength to materials, such as car parts, without adding weight. Already, the vimentin fiber's properties are comparable to human tendons, leading Pinto and Negishi to suggest that they might help replace damaged ones (5,6).…”

Section: Hagfish Inspirationmentioning

confidence: 99%

Will hagfish yield the fibers of the future?

Dance¹

2016

Proc. Natl. Acad. Sci. U.S.A.

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“…Balık asalakları başka birçok hayvanda da olduğu gibi her hangi bir tehlike anında vücutlarından mukusa benzer salgı salgılar [3,11,16,27]. Fakat bu salgıyı diğer hayvanların salgıladığı sıvıdan ayıran en önemli özellik, içerisinde gözlemlenen liflerdir.…”

Section: Balik Asalaklarinin Salgilarinda Bulunan Li̇fleri̇n Yapisiunclassified

“…Fakat bu salgıyı diğer hayvanların salgıladığı sıvıdan ayıran en önemli özellik, içerisinde gözlemlenen liflerdir. Bu lifler, balık asalağının salgı bezlerinin çeperinde sıralanan Salgı lifi hücreleri (Gland Thread Cells-GTCs) tarafından üretilir ve çok sayıda keratin-benzeri ara filament proteinlerinin bir araya gelmesi ile oluşur [16,24,28]. Devamlı bir yapıya sahip olan bu lifler birbiri üzerine sarılarak Şekil 3'te gösterilen ara lifler topluluğunu meydana getirirler [16,24,28].…”

Section: Balik Asalaklarinin Salgilarinda Bulunan Li̇fleri̇n Yapisiunclassified

Yarının Yüksek Performanslı Liflerine Doğal Bir Yaklaşım: Balık Asalağı Salgısı Lifleri

Kalayci

Avinç

Yavaş

2015

Mar. J. Pure Appl. Sci.

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ÖZET'Hiperotreti' olarak da bilinen balık asalakları kendilerini tehlike altında hissettiklerinde vücutları üzerinde bulunan salgı bezlerinden mukusa benzer bir sıvı salgılar. Bu salgı içerisinde bulunan lifler bugüne dek bilinen en tok lifler arasındadırlar. Yaklaşık 15 cm uzunluğunda ve ortalama 1-3 μm çapında olan bu lifler birçok doğal ve sentetik liften daha üstün özelliklere sahiptir. Balık asalağı salgısı lifleri yapısal özellikleri ile yün ve saç gibi α-keratin içeren diğer doğal protein liflerinden ayrılmaktadır. Kopma mukavemeti de yüksek olan balık asalağı salgısı lifleri oldukça iyi bir esneme kabiliyetine sahiptir. Henüz ticari olarak üretilemeyen bu lifler üzerinde araştırmalar halen devam etmektedir. Hagfish Slime Fibers ABSTRACT Hagfishes, also known as 'hyperotreti', release a mucus-like slime from their glands located on their body when they feel threatened. This slime contains fibers that are one of the toughest fibers known to date. Those fibers are approximately 15 cm long and 1-3 μm in width and have superior properties to most of natural and synthetic fibers. Hagfish slime fibers differ with their structural features from other natural protein fibers which contain α-keratin such as wool and hair. With a high breaking strength, these fibers also exhibit considerably good elongation. The hagfish slime fibers are not commercially manufactured yet while research is still in progress.

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“…Membrane peptides are often organized in bundles, whose structure is dominated by α-helical coiled-coils 20,[27][28][29][30] . Coiled coils consist of α-helices wind together to form a ropelike structure stabilized by hydrophobic interactions, and this structure is found in about 10% of the proteins in the human genome 31 .…”

mentioning

confidence: 99%

The Molecular Structure of Human Red Blood Cell Membranes From Highly Oriented, Solid Supported Multi-Lamellar Membranes

Himbert

Alsop

Rheinstädter

2018

Biophysical Journal

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We prepared highly oriented, multi-lamellar stacks of human red blood cell (RBC) membranes applied on silicon wafers. RBC ghosts were prepared by hemolysis and applied onto functionalized silicon chips and annealed into multi-lamellar RBC membranes. High resolution X-ray diffraction was used to determine the molecular structure of the stacked membranes. We present direct experimental evidence that these RBC membranes consist of nanometer sized domains of integral coiled-coil peptides, as well as liquid ordered (l o ) and liquid disordered (l d ) lipids. Lamellar spacings, membrane and hydration water layer thicknesses, areas per lipid tail and domain sizes were determined. The common drug aspirin was added to the RBC membranes and found to interact with RBC membranes and preferably partition in the head group region of the l o domain leading to a fluidification of the membranes, i.e., a thinning of the bilayers and an increase in lipid tail spacing. Our results further support current models of RBC membranes as patchy structures and provide unprecedented structural details of the molecular organization in the different domains.The presence of pale cells with no internal content in a blood smear is typically indicative of a disease. These cells are produced by hemolysis and have been named red blood cell (RBC) ghosts based on their appearance under the microscope. RBC ghosts can be prepared artificially [1][2][3] . The first published protocol in 1963 by Dodge, Mitchell and Hanahan describes the extraction of the cell membrane from RBCs through hemolysis and was an essential step in the development of membrane proteomics and lipidomics 4,5 . While there is detailed knowledge of the composition of RBC membranes, information about the molecular organization of these components in the actual membranes is scarce 6 . This is, in particular, a consequence of the lack of suitable experimental techniques. The disordered, patchy and highly dynamic state of biological materials impedes, for instance, the use of high-resolution diffraction techniques as in protein crystallography. To overcome this constraint, we prepared highly oriented stacks of RBC membranes on silicon wafers and studied their molecular properties in-vitro using high resolution X-ray diffraction. The results present evidence for nanometer-sized domains of coiled-coils peptides, as well as liquid ordered (l o ) and liquid disordered (l d ) lipid patches, and give a detailed picture of the molecular organization in these domains.When present in the body, aspirin (acetylsalicylic acid, ASA) and its metabolites interact with the cyclo-oxygenase (COX) pathway. The inhibition of both COX isoforms, COX-1 and COX-2, by higher dose aspirin is believed to lead to analgesic and anti-inflammatory effects, while lower doses, sufficient to inhibit COX-1 activity, lead to anti-platelet activity 7,8 . There is recent evidence that membrane composition and fluidity play an important role in platelet cell function [9][10][11] , possibly related to the formation of rafts 12 .

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Self-Assembly Enhances the Strength of Fibers Made from Vimentin Intermediate Filament Proteins

Cited by 47 publications

References 31 publications

Will hagfish yield the fibers of the future?

Will hagfish yield the fibers of the future?

Yarının Yüksek Performanslı Liflerine Doğal Bir Yaklaşım: Balık Asalağı Salgısı Lifleri

The Molecular Structure of Human Red Blood Cell Membranes From Highly Oriented, Solid Supported Multi-Lamellar Membranes

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