Self-assembled triangular DNA nanoparticles are an efficient system for gene delivery

Regulation on Toll-like Receptor 4 and Cell Barrier Function by Rab26 siRNA-loaded DNA Nanovector in Pulmonary Microvascular Endothelial Cells

“…A lot of non-viral vectors have been explored as gene delivery tools, such as cationic lipids 36, cationic polymer 37, dendrimers 38, inorganic nanoparticles 39 and even cell penetrating peptides 40. More recently, it has been consistently observed that self-assembled DNA nanoparticles with a simpler structure are highly effective at delivering siRNAs into mammalian cells and down-regulating the target gene 23. Moreover, compared with DNA origami-based complex DNA nanomaterials, particles with simpler structures have several advantages, including the ability to be more accurately programmed, higher productivity, a lower mismatch rate and lower cost 23.…”

“…More recently, it has been consistently observed that self-assembled DNA nanoparticles with a simpler structure are highly effective at delivering siRNAs into mammalian cells and down-regulating the target gene 23. Moreover, compared with DNA origami-based complex DNA nanomaterials, particles with simpler structures have several advantages, including the ability to be more accurately programmed, higher productivity, a lower mismatch rate and lower cost 23. Current RNAi strategies using duplex siRNAs suffer from low efficiency and a high cost, particularly for primary cells, and generally at least three different sequences against the target gene need to be designed to ensure knockdown success.…”

“…Recently, self-assembled DNA nanostructures have been demonstrated to have great potential for delivering drugs and oligonucleotides into cells, with high stability and transfection efficiency and low toxicity and immunogenicity 23. Our previous study showed that a delivery vehicle based on programmed self-assembly of mammalian target of rapamycin (mTOR)-siRNA-loaded DNA nanotubes (DNA-NTs) was effectively transfected into primary pulmonary arterial smooth muscle cells (PASMCs), induced obvious autophagy and inhibited cell growth under both normal and hypoxic conditions 24.…”

Targeted Delivery of Rab26 siRNA with Precisely Tailored DNA Prism for Lung Cancer Therapy

“…Due to the nature of DNA, this new class of material is sensibly considered a potential smart material platform as drug delivery vehicles, sensors and other functional units . Thus far, self‐assembled DNA nanostructures have been successfully explored as drug delivery vehicles for small molecule drugs, siRNA, proteins and other guest molecules in vitro or in vivo. Indeed, DNA nanostructures exhibited excellent stability in physiological settings compared to antisense DNA or RNA due to steric effects; meanwhile, they are readily taken up by cancerous cells and well‐enriched in tumors due to the enhanced permeability and retention (EPR) effect.…”

DNA nanostructure-based siRNA delivery systems

“…作者以两种DNA:mTOR-1A和mTOR-2A组建 图 6 (网络版彩色)基于DNA纳米结构的单条功能性siRNA递送体系. (a) 负载mTOR siRNA的纳米管递送系统 [75] ; (b) 三角形DNA-RNA杂交 体系的siRNA递送系统 [76] Figure 6 (Color online) DNA nanostructure-based carriers for single functional siRNA delivery. (a) Schematic illustration of the mTOR siR-NA-loaded nanotubes [75] ; (b) schematic of triangular DNA-RNA hybrid system for siRNA delivery [76]…”