Self-assembled, photoluminescent peptide hydrogel as a versatile platform for enzyme-based optical biosensors

Kim, Jae Hong; Lim, Seong Yoon; Nam, Dong Heon; Ryu, Jungki; Ku, Sook Hee; Park, Chan Beum

doi:10.1016/j.bios.2010.01.026

Cited by 124 publications

(53 citation statements)

References 36 publications

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“…Kim et al used a CdTe quantum dot embedded peptide hydrogel matrix for optical glucose sensing. The photoluminescence of the CdTe was reduced as the glucose concentration increased (Kim et al, 2011). In case of the enzyme mediated glucose detection, the immobilization of enzymes in a solid matrix such as nanomaterials (Zhang et al, 2004) and polymers (Ivekovic et al, 2004) has been investigated to achieve the enhanced enzymatic activity by avoiding the enzyme aggregation and the improved enzyme stability.…”

Section: Introductionmentioning

confidence: 99%

Enzyme incorporated microfluidic device for in-situ glucose detection in water-in-air microdroplets

Piao

Han

Azad

et al. 2015

Biosensors and Bioelectronics

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Section: Introductionmentioning

confidence: 99%

Enzyme incorporated microfluidic device for in-situ glucose detection in water-in-air microdroplets

Piao

Han

Azad

et al. 2015

Biosensors and Bioelectronics

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“…[21] Self-assembled dipeptides-such as photoluminescent Phe-Phe peptide nanotubes for optical, electrical, and biological applications, and an Fmoc-Phe-Phe (Fmoc = 9-fluorenylmethyl carbamate) peptide hydrogel-based optical biosensor for the detection of neurotoxins and compounds such as glucose and hydrogen peroxide-were developed. [22][23][24] The synthesis of transition-metal-phosphate nanotubes for rechargeable lithium-ion batteries by using Fmoc-Phe-Phe peptide hydrogel as a template has been reported. [25] Aromatic dipeptides have been demonstrated to self-assemble into discrete nanotubes that can serve as molds for conductive nanowires suitable for various applications.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Silver and Fluorescent Dyes Incorporated in Self‐Assembling Peptides and Their Potential Applications in Biology

Subbalakshmi

Nagaraj

2014

ChemPlusChem

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There is increasing interest in studying the interaction of nanoparticles with biomolecules, microorganisms, and noble metals to explore their unique properties and potential biological applications. The synthetic butyloxycarbonyl (Boc)‐protected methyl esters of peptides composed of only aliphatic amino acids (Boc‐Ile‐Ile‐OMe and Boc‐Leu‐Ile‐Ile‐OMe) self‐assembled into nanotubes and spheres, respectively, has been previously demonstrated. Herein, the characterization of these peptide nanoparticles incorporated with silver by means of various spectrometric methods and electron microscopy imaging techniques is described. The antibacterial activity of Ag‐doped nanoparticles was determined. These peptide nanoparticles were demonstrated to be useful encapsulants of charged fluorescent dyes. This investigation demonstrates the potential of these short peptide nanoparticles to design formulations of antimicrobial agents that will enhance their activity and as fluorescent probes for cell imaging.

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“…Current injectable hydrogels with photoluminescence are synthesized by mixing or conjugating hydrogels with semiconducting quantum dots [36, 37], carbon dots [35], rare-earth upconversion nanoparticles [38–40], or organic dyes [41–44]. Compared to organic dye conjugated hydrogels, those incorporated with semiconducting quantum dots, carbon dots, and rare-earth upconversion nanoparticles may have toxicity concern [45].…”

Section: Introductionmentioning

confidence: 99%

Thermosensitive, fast gelling, photoluminescent, highly flexible, and degradable hydrogels for stem cell delivery

Niu

et al. 2019

Acta Biomaterialia

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Stem cell therapy is a promising approach to regenerate ischemic cardiovascular tissues yet experiences low efficacy. One of the major causes is inferior cell retention in tissues. Injectable cell carriers that can quickly solidify upon injection into tissues so as to immediately increase viscosity have potential to largely improve cell retention. A family of injectable, fast gelling, and thermosensitive hydrogels were developed for delivering stem cells into heart and skeletal muscle tissues. The hydrogels were also photoluminescent with low photobleaching, allowing for non-invasively tracking hydrogel biodistribution and retention by fluorescent imaging. The hydrogels were polymerized by N-Isopropylacrylamide (NIPAAm), 2-hydroxyethyl methacrylate (HEMA), 1-vinyl-2-pyrrolidinone (VP), and acrylate-oligolactide (AOLA), followed by conjugation with hypericin (HYP). The hydrogel solutions had thermal transition temperatures around room temperature, and were readily injectable at 4°C. The solutions were able to quickly solidify within 7s at 37°C. The formed gels were highly flexible possessing similar moduli as the heart and skeletal muscle tissues. In vitro, hydrogel fluorescence intensity decreased proportionally to weight loss. After being injected into thigh muscles, the hydrogel can be detected by an in vivo imaging system for 4 weeks. The hydrogels showed excellent biocompatibility in vitro and in vivo, and can stimulate mesenchymal stem cell (MSC) proliferation and paracrine effects. The fast gelling hydrogel remarkably increased MSC retention in thigh muscles compared to slow gelling collagen, and non-gelling PBS. These hydrogels have potential to efficiently deliver stem cells into tissues. Hydrogel degradation can be non-invasively and real-time tracked.

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Self-assembled, photoluminescent peptide hydrogel as a versatile platform for enzyme-based optical biosensors

Cited by 124 publications

References 36 publications

Enzyme incorporated microfluidic device for in-situ glucose detection in water-in-air microdroplets

Enzyme incorporated microfluidic device for in-situ glucose detection in water-in-air microdroplets

Characterization of Silver and Fluorescent Dyes Incorporated in Self‐Assembling Peptides and Their Potential Applications in Biology

Thermosensitive, fast gelling, photoluminescent, highly flexible, and degradable hydrogels for stem cell delivery

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